Chinese Journal of Tissue Engineering Research ›› 2021, Vol. 25 ›› Issue (7): 1002-1007.doi: 10.3969/j.issn.2095-4344.2163

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Biological characteristics of canine adipose-derived mesenchymal stem cells cultured in hypoxia

Fan Quanbao1, Luo Huina1, Wang Bingyun1, Chen Shengfeng1, Cui Lianxu2, Jiang Wenkang1, Zhao Mingming1, Wang Jingjing1, #br# Luo Dongzhang1, Chen Zhisheng1, Bai Yinshan1, Liu Canying1, Zhang Hui1   

  1. 1College of Life Science and Engineering, Foshan University of Science and Technology, Foshan 528231, Guangdong Province, China; 2Foshan Hospital Affiliated to Sun Yat-sen University & Department of Neurosurgery, Foshan First People’s Hospital, Foshan 528000, Guangdong Province, China
  • Received:2020-01-17 Revised:2020-01-19 Accepted:2020-03-18 Online:2021-03-08 Published:2020-12-08
  • Contact: Chen Shengfeng, MD, Associate professor, College of Life Science and Engineering, Foshan University of Science and Technology, Foshan 528231, Guangdong Province, China Wang Bingyun, MD, Professor, College of Life Science and Engineering, Foshan University of Science and Technology, Foshan 528231, Guangdong Province, China
  • About author:Fan Quanbao, College of Life Science and Engineering, Foshan University of Science and Technology, Foshan 528231, Guangdong Province, China
  • Supported by:
    the Natural Science Foundation of Guangdong Province, No. 2017A030313171; the Natural Science Foundation of Guangdong Province, No. 2018A030313892

Abstract: BACKGROUND: Previous studies have found that hypoxia has different effects on the proliferation and differentiation of mesenchymal stem cells and secretion of cytokines, but the effect of hypoxia on canine adipose-derived mesenchymal stem cells has not been seen. 
OBJECTIVE: To investigate the effect of hypoxic environment on the biological characteristics of canine adipose-derived mesenchymal stem cells. 
METHODS:  Canine adipose-derived mesenchymal stem cells were isolated and cultured by enzyme digestion. Passage 2 adipose-derived mesenchymal stem cells could be divided into normoxia group (21% oxygen volume fraction) and hypoxia group (5% oxygen volume fraction). Morphological characteristics, proliferation speed, cell surface marker expression, differentiation capacity, and cytokine secretion level were compared between the two groups.  
RESULTS AND CONCLUSION: (1) The growth of adipose-derived mesenchymal stem cells cultured in hypoxic environment was good. The cell morphology was fusiform and polyhedral, the same as that of the normoxia group. (2) The proliferation rate of cells in the hypoxia group was accelerated, and the cell doubling time was shorter than that in the normoxia group (all P < 0.05). The differentiation time of lipogenesis and osteogenesis was shortened. (3) The expression of CD90, CD44 and CD105 was high in both normoxia group and hypoxia group, while the expression of CD45 was low. (4) The mRNA expression levels of brain-derived neurotrophic factor and vascular endothelial growth factor in the hypoxia group were 2.3 times (P < 0.05) and 3.0 times (P < 0.05) higher than those in the normoxia group. (5) The results indicated that the hypoxic environment had no significant effect on the morphology and surface marker of adipose-derived mesenchymal stem cells, but promoted cell proliferation, differentiation and cytokine secretion.

Key words: stem cell, adipose-derived mesenchymal stem cells, hypoxia, cytokine, biological characteristics, dog, experiment

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