Human cartilage glycoprotein-39 induces chondrogenesis of precartilaginous stem cells

doi:10.3969/j.issn.2095-4344.2015.42.013

Abstract

Abstract:

BACKGROUND: Studies have shown that human cartilage glycoprotein-39 has a certain relationship to articular cartilage degeneration and repair, but the mechanism of action is not very clear.
OBJECTIVE: To investigate the effect of human cartilage glycoprotein-39 on chondrogenesis of precartilaginous stem cells.
METHODS: Precartilaginous stem cells were isolated from the adult articular cartilage. Cells which could express CD105 and CD166 were detected using flow cytometry followed by isolation and purification. Isolated precartilaginous stem cells werecultured using monolayer method, and then, passage 2 cells were cultured in the medium containing human cartilage glycoprotein-39 and normal chondrogenic medium for 14 days, respectively. Immunohistochemical staining was used to observe expression of type II collagen and gross observation was
done for evaluation of cartilage formation.
RESULTS AND CONCLUSION: The precartilaginous stem cells isolated from the adult articular cartilage could express CD105 and CD166. After induction, differentiated precartilaginous stem cells gradually gathered and formed nudes. The induced cells were positive for type II collagen; after induction by human cartilage glycoprotein-39, the nodules became larger and the expression of type II collagen was increased. These findings indicate that precartilaginous stem cells with chondrogenic ability can be isolated from the adult articular cartilage, and can be induced to differentiate into chondrocytes, in which human cartilage glycoprotein-39 plays an important role.
中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Chondrocytes, Glycoproteins, Cartilage, Tissue Engineering

Chen Yue-ping, Dong Pan-feng, Yuan Zhen-zhong, Rao Yi, Li Jin-huan, Kang Jie, Zhang Xiao-yun. Human cartilage glycoprotein-39 induces chondrogenesis of precartilaginous stem cells[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(42): 6793-6797.

Figures/Tables 5

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