Chinese Journal of Tissue Engineering Research ›› 2015, Vol. 19 ›› Issue (42): 6798-6802.doi: 10.3969/j.issn.2095-4344.2015.42.014

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Significance of erythropoietin for the embryonic development of the lens

Guo Qing-min1, Meng Xu-xia1, Tian Ye2, Hu Die1   

  1. 1Department of Ophthalmology, Affiliated Hospital of Qingdao University, Qingdao 266555, Shandong Province, China; 2People’s Hospital of Economic and Technological Development Zone of Linyi City, Linyi 276000, Shandong Province, China
  • Online:2015-10-08 Published:2015-10-08
  • Contact: Meng Xu-xia, Master, Chief physician, Professor, Department of Ophthalmology, Affiliated Hospital of Qingdao University, Qingdao 266555, Shandong Province, China
  • About author:Guo Qing-min, Studying for master’s degree, Department of Ophthalmology, Affiliated Hospital of Qingdao University, Qingdao 266555, Shandong Province, China
  • Supported by:

    the Public Domain Technology Support Program of Qingdao City, No. 09-1-1-18-nsh

Abstract:

BACKGROUND: Studies have found that erythropoietin has a protective effect on embryonic development of the retina, but there are rare studies concerning the embryonic development of the lens.

OBJECTIVE: To observe the difference in erythropoietin expression in the lens from Wistar rats at different embryonic periods and to investigate the effect and significance of erythropoietin in the embryonic development of the lens.
METHODS: Clean Wistar rats with pregnancy for 10 days (n=5), 12 days (n=5), 14 days (n=5), 16 days (n=5), 18 days (n=5), 20 days (n=5) were randomly collected and divided into six groups. Every two embryonic ratsof the different 30 pregnant rats were obtained randomly by the caesarean operation under ketamine-induced anesthesia. The eye tissues of all the embryonic rats were isolated and cut into sections. The expression of erythropoietin protein and mRNA in rat lens was detected by immunohistochemistry and reverse t ranscription-PCR, respectively.

RESULTS AND CONCLUSION: Erythropoietin was distinctly expressed at the six different embryo stages, and the expression of erythropoietin protein and mRNA gradually increased from embryonic day 10 to embryonic day 16, and decreased from embryonic day 10 to embryonic day 20. There were significant differences between the six groups. These findings indicate that the expression of embryonic appears in a low to high to low fashion during the embryonic development of Wistar rats, which may be closely associated with the developing procedure of lens. 
中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程

Key words: Erythropoietin, Lens, Crystalline, Embryonic Development