Differentiation of neural stem cells induced by erythropoietin in vitro

doi:10.3969/j.issn.2095-4344.2015.45.022

Chinese Journal of Tissue Engineering Research ›› 2015, Vol. 19 ›› Issue (45): 7337-7341.doi: 10.3969/j.issn.2095-4344.2015.45.022

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Differentiation of neural stem cells induced by erythropoietin in vitro

Zhao Yan¹, Wang Xi-liang¹, Xiao Yu-long¹, Huo Hong-jun¹, Jiang Jian-ming², Yan Hui-bo³

1Department of Thoracolumbar Spine Surgery, the Second Affiliated Hospital of Inner Mongolia Medical University, Hohhot 010030, Inner Mongolia Autonomous Region, China; 2Department of Orthopedic and Spinal Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong Province, China; 3Department of Spinal Surgery, Third Affiliated Hospital, Southern Medical University, Guangzhou 510630, Guangdong Province, China

Received:2015-09-25 Online:2015-11-05 Published:2015-11-05
Contact: Xiao Yu-long, Chief physician, Department of Thoracolumbar Spine Surgery, the Second Affiliated Hospital of Inner Mongolia Medical University, Hohhot 010030, Inner Mongolia Autonomous Region, China
About author:Zhao Yan, M.D., Associate chief physician, Department of Thoracolumbar Spine Surgery, the Second Affiliated Hospital of Inner Mongolia Medical University, Hohhot 010030, Inner Mongolia Autonomous Region, China
Supported by:
the Natural Science Foundation of Scientific Committee of Inner Mongolia Autonomous Region, No. 2011MS1139

Abstract

Abstract:

BACKGROUND: In recent years, neural stem cells are considered to be ideal for the treatment of spinal cord injury, but the proportion of its natural differentiation into neurons in the host body is relatively low, which severely restricts the therapeutic effect on spinal cord injury.
OBJECTIVE: To investigate the effect of erythropoietin on the differentiation of neural stem cells in vitro.
METHODS: Under sterile condition, neural stem cells from the hippocampus of neonatal Wistar rats were isolated, cultured and identified by immunofluorescence in vitro. The third generation of neural stem cells were randomly divided into 0.5, 5, 50 U/mL erythropoietin groups and control group (with no erythropoietin).
RESULTS AND CONCLUSION: Compared with the control group, the differentiation rate of neural stem cells was significantly improved in the 0.5, 5, 50 U/mL erythropoietin groups (P < 0.05); moreover, the differentiation rate of neural stem cells in the 5, 50 U/mL erythropoietin groups was higher than that in the 0.5 U/mL erythropoietin group (P < 0.05). In addition, there was no significant difference between the 5, 50 U/mL erythropoietin groups (P > 0.05). These findings indicate that erythropoietin can effectively induce the differentiation of neural stem cells into neurons in vitro, and moreover, it can significantly improve the differentiation rate of neural stem cells into neurons.
中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Erythropoietin, Neural Stem Cells, Cell Differentiation, Tissue Engineering

Zhao Yan, Wang Xi-liang, Xiao Yu-long, Huo Hong-jun, Jiang Jian-ming, Yan Hui-bo. Differentiation of neural stem cells induced by erythropoietin in vitro[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(45): 7337-7341.

Figures/Tables 4

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Differentiation of neural stem cells induced by erythropoietin in vitro

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