Signal pathway effects on gastric cancer stem cells and gastric cancer occurrence

doi:10.3969/j.issn.2095-4344.2013.14.009

Abstract

Abstract:

BACKGROUND: The effect of Hedgehog and Wnt/β-Catenin signal pathway on gastric cancer stem cells and the occurrence and development of gastric cancer is rarely reported.
OBJECTIVE: To investigate the mechanism of Hedgehog and Wnt/β-Catenin signal pathway on occurrence of gastric cancer stem cells.
METHODS: Gastric cancer stem cells were selected from cell suspension in the culture system of serum-free medium by magnetic activated cell sorting system. Immunohistochemical SP test was applied to detect the expressions of SHH, GLI1, Wnt2 and β-Catenin protein in gastric cancer stem cells. The relationship among these factors was analyzed by Spearman.
RESULTS AND CONCLUSION: The expression positive rates of SHH, GLI1, Wnt2 and β-Catenin were 74.7%, 78.3%, 85.5% and 83.3% respectively. It was remarkably higher than that of tissues adjacent to cancer (P < 0.05). The expression of each factors had negative relationships (P < 0.05). Hedgehog and Wnt/β-Catenin signal pathway were stimulated abnormally in gastric cancer stem cells. These two pathways may have a co-relationship in gastric cancer occurrence. It provides a new research direction for the treatment of gastric cancer.

Key words: stem cells, tumor stem cells, gastric cancer, gastric cancer stem cells, Hedgehog signal pathway, Wnt/β-catenin signal pathway, SHH, GLI1, Wnt2, β-catenin, clone cells, immunohistochemistry, positive cells, stem cell photographs-containing paper

CLC Number:

R394.2

Chen Hao, Xu Lang. Signal pathway effects on gastric cancer stem cells and gastric cancer occurrence[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(14): 2532-2537.

Figures/Tables 3

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