Chinese Journal of Tissue Engineering Research ›› 2014, Vol. 18 ›› Issue (20): 3149-3152.doi: 10.3969/j.issn.2095-4344.2014.20.007

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Influence of geniposide on collagen II synthesis of cultured chondrocytes in vitro

Tan Yu-lin1, Bao Tong-zhu2, Liu Qin2, Han Yu3, Wang Yan-lin3, Liu Yang2, Zhou Na-xin2, Yan Fei2,Zhao Long-tao2   

  1. 1The First College of Clinical Medical Science, Three Gorges University, Yichang 443002, Hubei Province, China; 2Yichang Central People’s Hospital, Yichang 443002, Hubei Province, China;   3Institute of Molecular Biology, Medical College, Three Gorges University, Yichang 443002, Hubei Province, China
  • Received:2013-11-29 Online:2014-05-14 Published:2014-05-14
  • Contact: Bao Tong-zhu, Yichang Central People’s Hospital, Yichang 443002, Hubei Province, China
  • About author:Tan Yu-lin, Master, The First College of Clinical Medical Science, Three Gorges University, Yichang 443002, Hubei Province, China
  • Supported by:

    the Natural Science Foundation of Hubei Province in China, No. 2010CDB10701

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Abstract:

BACKGROUND: Chinese herb Cape Jasmine Fruit can activate the chondrocytes and geniposide is an important component in this herb.
OBJECTIVE: To investigate the effect of geniposide on collagen II synthesis in rat chondrocytes cultured in vitro.
METHODS: Rat chondrocytes were separated and cultured in vitro. The chondrocytes were then interfered with 25, 50 and 100 mmol/L geniposide. Normal control group was also set. Collagen II mRNA and protein expression was detected with semi-quantitative RT-PCR and western blot analysis, respectively.
RESULTS AND CONCLUSION: RT-PCR and western blot analysis results showed that, geniposide at 25, 50 and 100 mmol/L increased the collagen II mRNA and protein expression (P < 0.01). Geniposide can promote the synthesis of collagen II in rat chondrocytes cultured in vitro.



中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程


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Key words: 组织构建, 软骨组织工程, 京尼平苷, Ⅱ型胶原蛋白, 软骨细胞, 大鼠, 组织工程, 湖北省自然科学基金

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