Effect of Dll4 at different concentrations on the biological functions of extravillous trophoblasts

doi:10.3969/j.issn.2095-4344.2015.24.017

Abstract

Abstract:

BACKGROUND: Extravillous trophoblasts exert a crucial effect on early pregnancy. When extravillous trophoblasts function abnormally, many pregnancy diseases can develop, such as preeclampsia, fetal growth restriction and placental implantation.
OBJECTIVE: To investigate the effect of Dll4, a ligand of Notch signaling family, on biological functions of extravillous trophoblasts.
METHODS: Human extravillous trophoblast cell lines, HTR-8/SVneo, were cultured with human recombined protein Dll4 of different concentrations (0, 0.125, 0.25, 0.5, 1.0 mg/L). Cell viability was tested by cell counting kit-8 assay. Transwell assay was applied to examine the changes in cell migration and invasion ability.
RESULTS AND CONCLUSION: Dll4 could significantly improve migration and invasion abilities of extravillous trophoblasts, but had no effect on cell viability. In addition, Dll4 regulated the invasion ability of extravillous trophoblast in a dose-dependent manner. Above all, Dll4 can play an important role in normal pregnancy by regulating the biological functions of extravillous trophoblasts.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: 组织构建, 组织工程, 绒毛外滋养细胞, Dll4, 迁移, 侵袭

CLC Number:

R318

Luo Qing-qing. Effect of Dll4 at different concentrations on the biological functions of extravillous trophoblasts [J]. Chinese Journal of Tissue Engineering Research, 2015, 19(24): 3860-3864.

Figures/Tables 1

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