Activated expression of matrix metalloproteinase is involved in the proliferation of MC3T3-E1 cells induced by lipopolysaccharide

doi:10.3969/j.issn.2095-4344.2015.24.018

Abstract

Abstract:

BACKGROUND: Matrix metalloproteinases and their inhibitors are proteolytic enzymes contaning Zn+, and involved in extracellular matrix degradation and tissue remodeling of a variety of tissues.
OBJECTIVE: To observe the effects of matrix metalloproteinases in the proliferation of MC3T3-E1 cells induced by lipopolysaccharide.
METHODS: MC3T3-E1 cell line was divided into four groups randomly: control group, low-dose lipopolysaccharide group (1 μmol/L), moderate-dose lipopolysaccharide group (10 μmol/L), and high-dose lipopolysaccharide group (100 μmol/L). The proliferation rate in each group was analyzed. Matrix metalloproteinase 2, 3, 9 and matrix metalloproteinase inhibitor 1 and 2 expressions were detected.
RESULTS AND CONCLUSION: The proliferation rate was increased greatly after medication of lipopolysaccharide in time-dependent and concentration-dependent manners. Moreover, the expressions of matrix metalloproteinases and their inhibitors were apparently enhanced, and showed significant differences. Results indicate that the enhanced expressions of matrix metalloproteinases participated in the proliferation of MC3T3-E1 cells induced by lipopolysaccharide.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Lipopolysaccharides, Matrix Metalloproteinases, Osteoblasts

CLC Number:

R318

Yan Guang-hua, Qin Hua. Activated expression of matrix metalloproteinase is involved in the proliferation of MC3T3-E1 cells induced by lipopolysaccharide [J]. Chinese Journal of Tissue Engineering Research, 2015, 19(24): 3865-3869.

Figures/Tables 3

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