Protection against apoptosis of islet cells: a combination of Caspase and Cocktail protease inhibitors

doi:10.3969/j.issn.2095-4344.2015.37.012

Abstract

Abstract:

BACKGROUND: Currently, there are few reports on the effects of Caspase inhibitors combined with Cocktail protease inhibitors on isolation and purification of islet cells.
OBJECTIVE: To explore the effects of Caspase inhibitors and Cocktail protease as protectors on islet cells during isolation and purification process.
METHODS: New-born pigs were selected to separate, purify and culture the islet cells, and after 24 hours of culture, the specimens were divided into three groups: blank control group, experimental group A (Caspase inhibitors and Cocktail protease were added only during digestion process), and experimental group B (Caspase inhibitors and Cocktail protease were added both during digestion and culture processes). AO-EB staining was used to qualitatively observe cell morphology and apoptosis. Flow cytometry was employed to quantitatively detect cell viability and apoptosis.
RESULTS AND CONCLUSION: Percentages of β cells were 66.91% in blank control group, 84.58% in the experimental group A, and 87.15% in the experimental group B. The proportions of living cells, apoptotic cells and
dead cells were 56.52%, 16.15%, 21.25% in the blank control group, 62.27%, 14.66%, 14.47% in the experimental group A, and 73.09%, 6.83%, 10.28% in the experimental group B, respectively. These findings indicate that in the process of digestion and culture in vitro, Caspase inhibitors and Cocktail protease inhibitors can both obviously decrease the loss of cells, and increase the percentage of islet beta cells.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Caspases, Islets of Langerhans, Apoptosis

CLC Number:

R318

Tian Ting-ting, Zhao Shan-shan, Wang Qun, Ye Bin. Protection against apoptosis of islet cells: a combination of Caspase and Cocktail protease inhibitors[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(37): 5966-5971.

Figures/Tables 1

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