Pretreatment with ginkgo biloba extract 50 alleviates radiation-induced acute intestinal injury in mice

doi:10.12307/2021.037

Abstract

Abstract: BACKGROUND: Acute intestinal injury may be triggered by high doses of radiotherapy to the chest, pelvis, abdomen, and total body. Previous studies have shown that ginkgo biloba extract 50 (GBE50) has antioxidant and anti-inflammatory effects, but its role in the treatment of radiation-induced acute intestinal injury is yet unclear.
OBJECTIVE: To investigate the protective effect and mechanism of GBE50 against radiation-induced acute intestinal injury in mice.
METHODS: Twenty-seven mice were randomly divided into control group, radiation group and GBE50 treatment group, with nine rats in each group. Pathological changes of the jejunum were observed by hematoxylin-eosin staining. Apoptosis in crypts and villi was evaluated by TUNEL staining. The expressions and distributions of Ki67, olfactomedin 4 and lysozyme in small intestine tissue were detected by immunohistochemistry. The expressions of Claudin-1, Zonula Occludens-1 and Lgr5 in small intestine tissue were detected by western blot. Malondialdehyde content and superoxide dismutase activity in small intestine tissue were detected by corresponding kit methods.
RESULTS AND CONCLUSION: Compared with the radiation group, GBE50 pretreatment significantly reduced the pathological changes and cell apoptosis caused by radiation in mice, improved the regeneration ability of intestinal stem cells after radiation, and reduced the destruction of intestinal epithelial barrier and oxidative stress induced by radiation. Therefore, all the results indicate that GBE50 pretreatment can alleviate radiation-induced acute intestinal injury in mice.

Key words: ginkgo biloba extract 50, radiation, radiotherapy, acute intestinal injury, cell apoptosis, crypt, villus

CLC Number:

Zuo Zhenkui, Han Jiarui, Ji Shuling, He Lulu. Pretreatment with ginkgo biloba extract 50 alleviates radiation-induced acute intestinal injury in mice[J]. Chinese Journal of Tissue Engineering Research, 2021, 25(23): 3666-3671.

Figures/Tables 4

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