Regulatory mechanism of Shenshuai Yin on cell apoptosis in the kidney of chronic renal failure rats

doi:10.12307/2021.038

Abstract

Abstract: BACKGROUND: Previous studies in animals have explored the pathway of apoptosis in the endoplasmic reticulum after treatment with Shenshuai Yin for chronic renal failure. However, the mechanism of cell apoptosis through other pathways remains to be studied.
OBJECTIVE: To investigate the regulatory mechanism of renal cell apoptosis of chronic renal failure rats through Fas/FasL pathway.
METHODS: Sixty male Sprague-Dawley rats were randomly divided into a blank group, a model group, a Niaoduqing group and a Shenshuai Yin group. A rat model of chronic renal failure was established by intragastric administration of adenine suspension 200 mg/kg per day. Rats in the Shenshuai Yin group were given Shenshuai Yin 33.3 g/kg per day by gavage. Rats in the Niaoduqing group were given Niaoduqing granule 2.25 g/kg per day by gavage. Rats in the blank group and model group were given 0.9% sodium chloride injection 8 mL/kg by gavage. Treatments in each group were conducted once a day for 4 consecutive weeks. After treatment, serum creatinine and urea nitrogen levels were detected. Renal histopathological changes were observed by hematoxylin-eosin staining. Apoptosis in renal tissues was evaluated by TUNEL staining. The protein levels of Fas, FasL, Caspase8 and Caspase3 were detected by western blot. An approval by the Animal Ethics Committee of Liaoning University of Traditional Chinese Medicine was given with an approval No. 21000092017044.
RESULTS AND CONCLUSION: Compared to the blank group, the levels of serum creatinine and urea nitrogen were significantly increased in the model, Niaoduqing and Shenshuai Yin groups (P < 0.01), the apoptotic index of renal cells was significantly increased (P < 0.01), and the protein levels of Fas, FasL, Caspase3 and Caspase8 were significantly increased (P < 0.01). The above indexes of Niaoduqing group and Shenshuai Yin group were all significantly improved as compared to the model group (P < 0.01), but there was no significant difference between the Niaoduqing group and Shenshuai Yin group (P > 0.05). To conclude, Shenshuai Yin can reduce renal cell apoptosis and improve renal function in chronic renal failure rats. The mechanism may be related to the inhibition of Fas/FasL mediated apoptosis pathway by Shenshuai Yin.

Key words: chronic renal failure, Shenshuai Yin, apoptosis, Fas/FasL, rat, model, animal

CLC Number:

Zhang Liang, Ma Xiaoyan, Wang Jiahong. Regulatory mechanism of Shenshuai Yin on cell apoptosis in the kidney of chronic renal failure rats[J]. Chinese Journal of Tissue Engineering Research, 2021, 25(23): 3672-3677.

Figures/Tables 5

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