Microscopic changes of freeze-dried vessels

doi:10.3969/j.issn.2095-4344.2015.37.009

Abstract

Abstract:

BACKGROUND: Mechanical properties of the blood vessels include axial and radial tension which is important to guarantee the tissue perfusion and its pressure.
OBJECTIVE: To test and analyze stretching and puncture parameters that can fully characterize the performance of blood vessels, so as to predict the health condition of the vessels transplanted into recipients.
METHODS: Mechanical properties as circumferential tension, axial tension and puncture were tested on the porcine aorta, and then pathological and staining analysis was done to explore whether freeze-dried vessels can withstand the blood pressure in all directions after transplanted into the body.
RESULTS AND CONCLUSION: Specific trend on the curves of freeze-dried vessels during circumferential tension, axial tension and puncture process was closer to that of fresh vessel curves, suggesting that structural components and arrangement of the freeze-dried vessels were not changed greatly. Meanwhile, the microscopic observations of slice and tensile section were taken into account so that the reason was studied and explained for the mechanical properties of the experimental data and results. The macroscopic mechanical result was analyzed based on the change of microstructure. These findings indicate that the vacuum freeze-drying is a better method to maintain the performance of blood vessels.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Freeze Drying, Freeze Drying, Tensile Strength

CLC Number:

R318

Xu Jie-yuan, Zhuang Mei-ping, Chen Su-fang, Lin Jing, Hong Cai-hong, Liu Meng-fang, Tao Le-ren. Microscopic changes of freeze-dried vessels[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(37): 5950-5954.

Figures/Tables 3

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