Expression of neuronal cadherin and placental cadherin in facial motoneurons after facial nerve injury

doi:10.3969/j.issn.2095-4344.2015.37.014

Abstract

Abstract:

BACKGROUND: Peripheral facial nerve injury first involves the retrograde reactions of central nervous system axons, and nerve regeneration will depend on the survival and functional status of neuronal cell bodies.

OBJECTIVE: To explore the expression of neuronal cadherin and placental cadherin in facial nuclei after facial nerve injury.

METHODS: New Zealand white rabbits were randomly divided into model group (n=48) and control group (n=8). In the model group, every eight rabbits were used to carry out the test respectively at 1, 4, 7, 14, 21 days after the model of facial nerve injury (right side) was established. SP and real-time quantitative PCR methods were taken to test the expression of neuronal cadherin and placental cadherin in the facial nerve nucleus at mRNA and protein levels.

RESULTS AND CONCLUSION: The control group had neuronal cadherin- and placental cadherin-positive neurons. In the model group, neuronal cadherin and placental cadherin positively expressed in the facial motorneurons (right side), and their expressions were peaked at 14 days. Compared with the control group, the mRNA expression of neuronal cadherin in the facial motorneurons was increased significantly at 4-28 days after
injury; the mRNA expression of placental cadherin in the facial motorneurons was decreased significantly at 1 day after injury, and then increased significantly at 7-28 days. It is suggested that the expression of neuronal cadherin and placental cadherin is positive in the early stage of facial nerve injury, and the expression of placental cadherin is always present, while the expression of neuronal cadherin relatively lasts for a short time. After facial nerve injury, the expression of neuronal cadherin and placental cadherin in the facial nerve nucleus is both increased, which indicates that the facial nerve regeneration may be related to the high expression of adhesion molecules.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Cadherins, Facial Nerve Injuries, Immunochemistry, Polymerase Chain Reaction

CLC Number:

R318

Li Lei-ji, Xu Chao-ran, Qin Gang, Liu Yue-hua, Zhu Lin. Expression of neuronal cadherin and placental cadherin in facial motoneurons after facial nerve injury[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(37): 5978-5982.

Figures/Tables 1

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