Chinese Journal of Tissue Engineering Research ›› 2014, Vol. 18 ›› Issue (28): 4563-4567.doi: 10.3969/j.issn.2095-4344.2014.28.022
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Liu Chuan1, Zou Ye-qing1, Shi Qing-zhi2
Online:
2014-07-02
Published:
2014-07-02
Contact:
Liu Chuan, Master, Associate chief technician, Laboratory of Molecular Medicine, the Second Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, China
About author:
Liu Chuan, Master, Associate chief technician, Laboratory of Molecular Medicine, the Second Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, China
CLC Number:
Liu Chuan, Zou Ye-qing, Shi Qing-zhi. Human cytomegalovirus detection in hematopoietic stem cell transplantation: value of fluorescence quantitative PCR in the early diagnosis[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(28): 4563-4567.
2.1 造血重建及植入情况 41例患者均获造血重建。中性粒细胞植活的中位时间为+11(+10至+16) d,血小板植活的中位时间为+17(+15至+24)d。所有患者均于移植后+30 d行骨髓穿刺检查,骨髓象为增生活跃或明显活跃,STR检测结果为完全供者嵌合。不同血型适时转变为供者血型。移植后+30 d,41例达完全缓解标准,+365 d 32例均达到完全缓解标准。2.2 实时荧光定量PCR检测结果 Real-time PCR共检测41例造血干细胞移植患者656份血标本,其中12例共96份标本阳性,人巨细胞病毒载量介于5.0×102-1.0×107 IU/mL之间。对照组2份标本阳性,人巨细胞病毒载量介于5.0×102- 1.0×103 IU/mL之间。移植组平均为(4.69±1.54)×105 IU/mL, 对照组平均为(11.6±2.21)×102 IU/mL。移植组与对照组均值比较采用t 检验,P < 0.01(t=2.087),差异有显著性意义(表1)。"
2.3 人巨细胞病毒DNA检测与人巨细胞病毒感染的治疗 Real-time PCR共检出12例患者阳性,人巨细胞病毒载量在5.0×102-1.0×107 IU/mL之间,其中7例患者表现为连续PCR阳性,伴DNA拷贝数逐渐升高,给予更昔洛韦治疗,患者均在一两周后DNA转阴,但仍有5例患者停药两三周后复发。其中5号患者共复发4次,第4次复发时表现为对更昔洛韦耐药,DNA不能转阴,很快死亡;16号患者第3次复发改为磷甲酸钠后DNA转阴,3,9,18,26,31号患者表现为非连续人巨细胞病毒DNA阳性,人巨细胞病毒载量未超过1.0×104 IU/mL,给予阿昔洛韦预防治疗,5例患者均未发展为人巨细胞病毒疾病(表2)。"
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