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    02 July 2014, Volume 18 Issue 28 Previous Issue    Next Issue
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    Differentiation of bone marrow mesenchymal stem cells into chondrocyte-like cells under the conditions of transforming growth factor beta and cyclical tensile strain
    Hao Yao, Qiao Liang, Hao Yong-zhuang, Xiang Chuan
    2014, 18 (28):  4429-4436.  doi: 10.3969/j.issn.2095-4344.2014.28.001
    Abstract ( 247 )   PDF (480KB) ( 444 )   Save

    BACKGROUND: Transforming growth factor-β has been shown to exert an obvious induction effect on the differentiation of bone marrow mesenchymal stem cells into chondrocytes. Cyclical tensile strain simulates mechanical environment of chondrocytes in the body, and plays an important regulatory role in cell proliferation and differentiation.
    OBJECTIVE: To discuss the synergy of transforming growth factor-β and cyclical tensile strain in inducing the differentiation of bone marrow mesenchymal stem cells into chondrocyte-like cells.
    METHODS: A total of 10 2-month-old New Zealand rabbits were selected. Bone needle was used to penetrate the medullary cavity of bone. 3.0-4.0 mL of bone marrow was extracted for isolation and culture of bone marrow mesenchymal stem cells. Passage 3 cells were randomly assigned to four groups: blank, transforming growth factor-β, cyclical tensile strain and cyclical tensile strain + transforming growth factor-β groups. After 1, 3 and 6 days, cells were obtained. General morphology was observed using safranin O staining. Glycosaminoglycan levels were detected by alcian blue staining. Matrix metalloproteinase-13 and tissue inhibitor of metalloproteinase-1 levels in supernatant were measured using ELISA. Type II collagen, matrix metalloproteinase-13 and tissue inhibitor of metalloproteinase-1 mRNA relative expression was detected using RT-PCR.
    RESULTS AND CONCLUSION: Safranin O staining showed fusiform or irregular triangular cells. Cell number  and matrix secretion increased in each experimental group than in blank group. Glycosaminoglycan levels in the supernatant were greater in the transforming growth factor-β and cyclical tensile strain + transforming growth factor-β groups than in the blank group (P < 0.05). Type II collagen mRNA relative expression was higher in the cyclical tensile strain + transforming growth factor-β group than in the blank group (P < 0.05). Results indicated that transforming growth factor-β and cyclical tensile strain could induce the differentiation of bone marrow mesenchymal stem cells into chondrocytes, showing an apparent cooperative action.


    中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


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    Interleukin-1 beta affects the biological properties of rat nucleus pulposus-derived mesenchymal stem cells
    Zhang Ning, Guan Xiao-ming, Ma Xun, Zhang Li, Zhang Hui, Song Liang
    2014, 18 (28):  4437-4443.  doi: 10.3969/j.issn.2095-4344.2014.28.002
    Abstract ( 280 )   PDF (535KB) ( 381 )   Save

    BACKGROUND: Endogenous stem cells have no repair effects on the process of disc degeneration. Authors assumed that this maybe associate with abnormal effects of related etiological factor, resulting in an inhibitory effect on the function of nucleus pulposus-derived mesenchymal stem cells.
    OBJECTIVE: To investigate the effects of inflammatory cytokine interleukin-1β on biological characteristics of nucleus pulposus-derived mesenchymal stem cells of rats.
    METHODS: Lumbar spinal nucleus pulposus was obtained from 3-month-old male Sprague-Dawley rats. Nucleus pulposus-derived mesenchymal stem cells were isolated and cultured with collagenase and sequential trypsin digestion. The expression of CD24, CD34, CD45, CD90 and CD105 was detected using flow cytometry. Stem cell gene SOX2 and Nanog expression was measured using RT-PCR. Adipogenic, osteogenic and chondrogenic abilities of nucleus pulposus-derived mesenchymal stem cells were observed. The apoptotic rate of interleukin-1β-treated nucleus pulposus-derived mesenchymal stem cells was detected using flow cytometry. Fluorescent quantitative PCR was used to measure the expression of SOX9, proteoglycan, type II collagenase 
    and caspase-3 gene after nucleus pulposus-derived mesenchymal stem cells were treated with interleukin-1β.
    RESULTS AND CONCLUSION: Cells isolated and cultured in vitro showed spindle-like fusiform. Nucleus pulposus-derived mesenchymal stem cells were positive for CD90 and CD105, negative for CD45, CD34 and CD24. Nucleus pulposus-derived mesenchymal stem cells expressed SOX2 and Nanog, and were able to differentiate into osteocytes and chondrocytes, but not adipocytes. By the cell counting kit-8, interleukin-1β inhibited the proliferation capacity of nucleus pulposus-derived mesenchymal stem cells, showing a dependent relationship with action time and action concentration. The optimum reaction time was 48 hours, and the optimal concentration was 50 μg/L. Flow cytometry detected that interleukin-1β induced a slight apoptosis of nucleus pulposus-derived mesenchymal stem cells. Fluorescent quantitative PCR results revealed that mRNA expression of SOX9, proteoglycan, and type II collagenase was decreased, but caspase-3 was increased. Results indicated that interleukin-1β inhibited the proliferation of nucleus pulposus-derived mesenchymal stem cells and induced the apoptosis of nucleus pulposus-derived mesenchymal stem cells, and was a reason that intervertebral own stem cells could not exert repair effects.


    中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


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    Changes in apoptosis-related genes in bone marrow mesenchymal stem cells after cocultured with hepatic stellate cells
    Hu Kun-peng, Liu Bo, Yao Zhi-cheng, Lin Ji-zong, Deng Mei-hai, Pan Wei-dong, Lin Nan, Chen Cheng, Xu Rui-yun
    2014, 18 (28):  4444-4449.  doi: 10.3969/j.issn.2095-4344.2014.28.003
    Abstract ( 284 )   PDF (842KB) ( 548 )   Save

    BACKGROUND: Previous studies have confirmed that bone marrow mesenchymal stem cells in vitro can promote hepatic stellate cell apoptosis and inhibit its activity, in which the mechanism of action remains unknown.
    OBJECTIVE: To screen out apoptosis-related genes during hepatic stellate cell apoptosis regulated by bone marrow mesenchymal stem cells using gene chip technology.
    METHODS: Purified human bone marrow mesenchymal stem cells were seeded in 6-well Transwell plate and cocultured with hepatic stellate cells. Cultured human bone marrow mesenchymal stem cells alone served as control group, and cultured for 72 hours. The alterations in apoptosis-related genes were analyzed between culture alone group and coculture group using gene chip technology. The genes strongly associated with regulation of hepatic stellate cells were selected. RESULTS AND CONCLUSION: By the functional classification of second-generation SABiosciences Gene chips, apoptotic gene screening found that after coculture, significantly upregulated genes in bone marrow mesenchymal stem cells contained: AKT1, PIK3R2, DAPK1, DHCR24, NOTCH2 and BDNF. Combined with previous findings, we hypothesized that NOTCH may play a key role in the regulation of hepatic stellate cells by bone marrow mesenchymal stem cells.


    中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


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    Influences of co-culture with primary bone marrow stromal cells on imatinib sensitivity and cell cycles of K562 cells  
    Wang Ji-gang, Zhou Fan, Liu Yan-qin, Bai Ying, Liu Jing-hua, Wu Dan-tong
    2014, 18 (28):  4450-4454.  doi: 10.3969/j.issn.2095-4344.2014.28.004
    Abstract ( 274 )   PDF (631KB) ( 470 )   Save

    BACKGROUND: Leukemia cells can obtain drug resistance phenotype mediated by adhesion to bone marrow stromal cells. But, for chronic myelogenous leukemia with adhesion functional defects, the role and mechanism of bone marrow stromal cells in imatinib-resistant formation remain unclear.
    OBJECTIVE: To construct the co-cultured model of bone marrow stromal cells–K562 cells and to investigate the influences of the co-culture with bone marrow stromal cells from the patients with chronic myelogenous leukemia on imatinib sensitivity of K562 cells and cell cycle.
    METHODS: The co-culture model was constructed by co-culturing K562 cells with bone marrow stromal cells isolated and cultured from the patients with chronic myelogenous leukemia. The IC50 values of K562 cells exposed to imatinib were quantified by MTT assay. The apoptotic rates of K562 cells exposed to 0.5 μmol/L imatinib for 72 hours were detected by flow cytometry through Annexin V-FIT/PI labeling. The cell cycles, cell cycle protein (cyclin A, cyclin D1 and cyclin E) expression of K562 cells co-cultured with bone marrow stromal cells for 72 hours were analyzed by flow cytometry.
    RESULTS AND CONCLUSION: The IC50 values of co-culture group and suspension culture group were respectively (0.52±0.02) μmol/L and (1.27±0.05) μmol/L, and their comparison showed significant differences (P < 0.01). After 72 hours of treatment with 0.5 μmol/L imatinib, the apoptotic rates in the co-culture group and suspension culture group were respectively (15.48±4.17)% and (32.01±6.83)%, and their comparison showed significant differences (P < 0.01). The percentages of G0-G1 phase of K562 cells co-cultured with bone marrow stromal cells for 72 hours were (48.81±8.27)%, which were significantly higher than the suspension culture group (25.78±3.26%) (P < 0.01). The co-culture with bone marrow stromal cells from the patients with chronic myelogenous leukemia could mediate K562 cells resistance to imatinib. The mechanism was possibly related with G0/G1 arrest of K562 cells induced by co-culture with bone marrow stromal cells.


    中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


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    Expression of osteogenic genes in rat bone marrow mesenchymal stem cells infected by lentivirus carrying hypoxia-inducible factor-1 alpha 
    Fu Zhi-jie, Zhang Ju-feng, Wang Da-ping, Chen Jie-lin, Duan Li, He Mei-jian, Li Qing-qing, Li Wen-cui, Xiong Jian-yi
    2014, 18 (28):  4455-4462.  doi: 10.3969/j.issn.2095-4344.2014.28.005
    Abstract ( 682 )   PDF (640KB) ( 792 )   Save

    BACKGROUND: Human hypoxia-inducible factor-1 alpha can regulate the expression of osteogenic and angiogenic genes, and promote osteogenic activity.
    OBJECTIVE: To observe the expression of osteogenic genes in rat bone marrow mesenchymal stem cells carrying human hypoxia-inducible factor-1 alpha slow virus infection.
    METHODS: Hypoxia-inducible factor-1 alpha was obtained from Hela cells using RT-PCR. Lentivirus expression vector plasmid carrying hypoxia-inducible factor-1 alpha (Lenti-HIF-1α-eGFP) was constructed. 293Ta cells with LentiPac HIV mixed packaging plasmid was packaged, and then lentivirus was obtained. Rat bone marrow mesenchymal stem cells were isolated and cultured using direct whole bone marrow adherent method. Bone marrow mesenchymal stem cells were identified using flow cytometry. Bone marrow mesenchymal stem cells were infected with slow virus for 1, 4, 7 and 14 days. Bone morphogenetic protein-2, osteocalcin, osteopontin and alkaline phosphatase expression levels were detected in bone marrow mesenchymal stem cells using real-time  fluorescent quantitative PCR.
    RESULTS AND CONCLUSION: Bone marrow mesenchymal stem cells were effectively infected with Lenti-HIF-1α-eGFP. Real-time fluorescent quantitative PCR results revealed that bone morphogenetic protein-2, osteocalcin, osteopontin and alkaline phosphatase began to obviously overexpress from 4 days after infection with Lenti-HIF-1α-eGFP until 14 days. Results suggested that hypoxia-inducible factor-1 alpha could elevate the osteogenic activity of bone marrow mesenchymal stem cells.


    中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


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    Isolation and culture of rat bone marrow mesenchymal stem cells using density gradient centrifugation and adherence separation screening
    Wang Ying-hui, Zheng Rui, Chen Li
    2014, 18 (28):  4463-4468.  doi: 10.3969/j.issn.2095-4344.2014.28.006
    Abstract ( 271 )   PDF (541KB) ( 436 )   Save

    BACKGROUND: Bone marrow mesenchymal stem cell content is less under normal conditions, and easily confounded with other cells. Therefore, to establish a simple feasible in vitro cultured amplification method and to obtain a large number of stable bone marrow mesenchymal stem cells are of important theoretical significance and application value.
    OBJECTIVE: To establish a simple feasible in vitro culture and amplification method and to obtain numerous stable bone marrow mesenchymal stem cells.
    METHODS: Bone marrow mesenchymal stem cells were isolated from Sprague-Dawley rats and cultured in vitro using density gradient centrifugation and adherence separation screening. Cell morphological changes were observed under an inverted light microscope. Submicroscopic structure of cells was observed under a transmission electron microscope. Living cell number was counted using Trypan blue exclusion method. Cell growth curve was drawn. Cell cycle was analyzed using flow cytometry. The expression of c-kit and CD45 was measured using immunocytochemistry. CD45 expression was analyzed using flow cytometry.
    RESULTS AND CONCLUSION: (1) Cell adhesion and pseudopodia could be seen under an inverted light microscope at 24 hours after inoculation; cell colony formed at 4 days; 90% cell confluence was found at 14 days. After passage, the cells were tended to homogenous and became fibroblast-like cells, which showed whirl-like growing or flamboyancy growing. (2) The size of passage 3 bone marrow mesenchymal stem cells was small and nucleolus was large, with clear nucleoli under a transmission electron microscope, with the presence of sparse chromatin and low electron density. There was microvillus on the surface of cells, abundant ribosomes could be seen in the cytoplasm with few other cellular organs such as endoplasmic reticulum, mitochondria and Golgi complex. These showed that ultrastructural structure had undifferentiated features. (3) The number of passage 3 bone marrow mesenchymal stem cells was reduced at 1 day after inoculation. The cells began to grow at 2 days, and entered the period of exponential growth at 3 days, entered the flat period at 7 days and the number of cells began to decrease at 9 days. Growth curve exhibited “S” shape. (4) The percentage of S phase cells was 21.1% as detected by flow cytometry after passage 3 bone marrow mesenchymal stem cells were stained by DNA. (5) Immunocytochemistry and flow cytometry had proved that the c-kit positive rate of cells was 53.3% and CD45 positive rate was 1.68%. (6) After osteogenic induction of mesenchymal stem cells for 16 days, cells became oval, and short processes connected each other. The cytoplasm was dark, suggesting that it may be rich in rough endoplasmic reticulum and Golgi complex. They can secrete osteoid. Results verified that obtained bone marrow mesenchymal stem cells stably grew, and actively proliferated.


    中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


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    Changes in memory function of rats with brain injury after fingolimod administration combined with bone marrow mesenchymal stem cell transplantation
    Wang Cheng
    2014, 18 (28):  4469-4473.  doi: 10.3969/j.issn.2095-4344.2014.28.007
    Abstract ( 361 )   PDF (412KB) ( 475 )   Save

    BACKGROUND: The repair effects of bone marrow mesenchymal stem cell transplantation on brain injury were not ideal. Combined therapy with medicine and biological engineering materials is needed.
    OBJECTIVE: To investigate the effects of bone marrow mesenchymal stem cell transplantation and fingolimod immunosuppressants on memory function recovery in rats with brain injury.
    METHODS: A total of 60 healthy Sprague-Dawley rats were subjected to hydraulic shock with peak value of 253.312 5–303.975 kPa with a hydraulic head injury instrument so as to induce a model of severe hydraulic head injury. They were randomly divided into brain injury group, bone marrow mesenchymal stem cell transplantation group and fingolimod + bone marrow mesenchymal stem cell transplantation group. The Morris water maze test was tested at 21–28 days after PKH-26-labeled bone marrow mesenchymal stem cell transplantation. The PKH-26 immunofluorescence and hematoxylin-eosin staining were conducted in brain tissues at 4 weeks after brain injury.
    RESULTS AND CONCLUSION: At 4 weeks after transplantation, the average escape latency was gradually decreased in each group. The average escape latency was shorter in the fingolimod + bone marrow mesenchymal stem cell transplantation group than in the bone marrow mesenchymal stem cell transplantation group (P < 0.05), and significantly shorter than in the brain injury group (P < 0.01). The number of times of crossing the platform and the percentage of swimming distance to total distance were higher in the fingolimod + bone marrow mesenchymal stem cell transplantation group than in the brain injury group and bone marrow mesenchymal stem cell transplantation group (P < 0.05). The number of PKH-26-positive cells was significantly higher in the fingolimod + bone marrow mesenchymal stem cell transplantation group than in the brain injury group and bone marrow mesenchymal stem cell transplantation group (P < 0.05). Results confirmed that bone marrow mesenchymal stem cell transplantation could apparently improve memory function of rats with severe  brain injury. The combined application of fingolimod immunosuppressants has synergistic effects.


    中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


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    Role of autophagy in human mesenchymal stem cells in response to irradiation
    Chen Zhe, Liu Jie, Zhang Bin
    2014, 18 (28):  4474-4478.  doi: 10.3969/j.issn.2095-4344.2014.28.008
    Abstract ( 295 )   PDF (603KB) ( 412 )   Save

    BACKGROUND: Bone marrow mesenchymal stem cells can survive under the lethal dose of radiation in response to hematopoietic stem cells, and still maintain the typical characteristics of stem cells to promote hematopoietic recovery after radiation. However, autophagy is an important mechanism for cellular adaptation under stress, which may be involved in radiation tolerance of bone marrow mesenchymal stem cells.
    OBJECTIVE: To investigate the role of autophagy in human bone marrow mesenchymal stem cells in response to irradiation.
    METHODS: Passage 3 bone marrow mesenchymal stem cells cultured in vitro at logarithmic phase were collected and randomized into control, 3-mehyladenine, rapamycin, irradiation, irradiation+3-mehyladenine and irradiation+rapamycin groups. The autophagy reactions of bone marrow mesenchymal stem cells were regulated by 5 mmol/L 3-mehyladenine and 200 nmol/L rapamycin for 12 hours in the 3-mehyladenine and rapamycin groups, respectively. Two-hour 6 Gy X-ray irradiation was performed in the irradiation group and two complication groups undergoing 12-hour corresponding drug intervention.
    RESULTS AND CONCLUSION: The proportions of cells with autophagic vacuoles and apoptotic cells were higher in the irradiation group than the control group, moreover, autophagic+apoptotic cells were increased in the irradiation group. 3-mehyladenine intervention could decrease the proportion of cells with autophagic vacuoles, and increase the number of apoptotic cells. But there was no difference in the proportion of autophagic+apoptotic cells between the 3-mehyladenine and irradiation groups. After rapamycin intervention, the proportion of autophagic cells was higher than that in the irradiation group, but no difference in the proportion of apoptotic cells between the two grups, as well as there were no autophagic+apoptotic cells. The expression of microtubule-associated protein 1 light chain 3-II was ranked as follows: the control and 3-mehyladenine groups < irradiation+3-mehyladenine group < irradiation group < rapamycin group < irradiation+rapamycin group. These findings indicate that autophagy induced by irradiation could contribute to protecting bone marrow mesenchymal stem cells from irradiation injury.


    中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


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    Cultivation and differentiation of human umbilical cord-derived mesenchymal stem cells treated with amphotericin B
    Huang Wen-jing, Liu Jun-jiang, Zhou Jian-yu, Hong Jing-xin, Li Qian, Han Jun-ling
    2014, 18 (28):  4479-4484.  doi: 10.3969/j.issn.2095-4344.2014.28.009
    Abstract ( 388 )   PDF (974KB) ( 600 )   Save

    BACKGROUND: High-quality and efficient umbilical cord-derived mesenchymal stem cells could be obtained by establishing and improving the method to avoid fungal contamination and by effectively decreasing pollution rate of isolated culture during umbilical cord collection.
    OBJECTIVE: To explore the effects of amphotericin B on growth and differentiation potential of umbilical cord-derived mesenchymal stem cells.
    METHODS: Umbilical cord-derived mesenchymal stem cells were separated from healthy full-termed delivery fetus using collagenase digestion method and treated with amphotericin B. Subsequently, umbilical cord-derived mesenchymal stem cells were amplified and cultured in vitro with MesenPRO RSTM medium. The third passage of umbilical cord-derived mesenchymal stem cells in logarithmic phase was obtained to analyze their morphology, proliferation and immunophenotype, and induced to differentiate into osteoblasts and adipocytes in vitro.
    RESULTS AND CONCLUSION: After treatment with amphotericin B, umbilical cord-derived mesenchymal stem cells were successfully isolated and cultured in vitro. Flow cytometry results revealed that human umbilical cord-derived mesenchymal stem cells strongly expressed CD44, CD105 and CD73, CD90, and negatively expressed HLA-DR, CD29, CD31, and CD34. Amphotericin B-treated human umbilical cord-derived mesenchymal stem cells can still differentiate into adipocytes and osteoblasts in vitro.


    中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


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    Vein transplantation of human umbilical cord-derived mesenchymal stem cells for treatment of hepatic fibrosis in rats
    Zhang Ying-jie, Li Yu-yun, Hao Xiao-na, Hao Yan-mei
    2014, 18 (28):  4485-4490.  doi: 10.3969/j.issn.2095-4344.2014.28.010
    Abstract ( 292 )   PDF (928KB) ( 344 )   Save

    BACKGROUND: Recent studies verified that other tissues-derived stem cells can be homing to the liver, possibly participate in the regeneration of liver tissues, which provides new hope for stem cells in treatment of liver disease.
    OBJECTIVE: To observe isolation and culture of human umbilical cord-derived mesenchymal stem cells, to observe the repairing effect of umbilical cord-derived mesenchymal stem cell transplantation on hepatic fibrosis, and then to provide a reliable theoretical basis for further clinical application of umbilical cord-derived mesenchymal stem cells.
    METHODS: Human umbilical cord-derived mesenchymal stem cells were isolated and purified by natural adherent method, and then cultured and amplified in vitro. A rat model of hepatic fibrosis was prepared using subcutaneous multi-point injection of CCl4. A total of 22 rat models were randomly assigned to model injury group (n=11) and cell transplantation group (n=11). At 1, 2 and 3 weeks after model induction, the rats in the cell transplantation group were treated with 1×106 umbilical cord-derived mesenchymal stem cells via caudal vein. Four weeks later, the rats were sacrificed. Blood of rats was collected from each group to detect liver functions. The liver was removed to receive hematoxylin-eosin staining, and pathological changes were observed. The number and distribution of Kupffer’s cells were observed using immunohistochemistry. The localization of umbilical cord-derived mesenchymal stem cells from treatment group was observed using immunohistochemistry.
    RESULTS AND CONCLUSION: After umbilical cord-derived mesenchymal stem cells were infused into rats with 
    cirrhosis via caudal vein, liver function was significantly improved, which showed significant differences as compared with the control group (P < 0.05). Hematoxylin-eosin staining revealed that hepatic fibrosis was apparently improved. Immunohistochemistry results demonstrated that the number of Kupffer’s cells was obviously reduced, and BrdU-labeled umbilical cord-derived mesenchymal stem cells were visible in rat liver of the treatment group using anti-BrdU antibody. These results suggested that umbilical cord-derived mesenchymal stem cell transplantation could improve biochemical characteristics of rat peripheral blood and histological structure of liver.


    中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


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    Differentially expressed long non-coding RNAs in adipogenic differentiation of human adipose-derived stem cells
    Yue Wen-jun, Wang Xiang-mei, Zhang Qin, Huang Hai-hua, Wei You-wan, Peng Zhi
    2014, 18 (28):  4491-4497.  doi: 10.3969/j.issn.2095-4344.2014.28.011
    Abstract ( 337 )   PDF (636KB) ( 481 )   Save

    BACKGROUND: The obesity has led to a plenty of diseases including hypertension, coronary heart disease, fatty liver, hyperlipidemia, and type 2 diabetes. Therefore, understanding the mechanism of adipocyte differentiation is of far-reaching significance to the prevention and treatment of obesity. For the current studies of the mechanism of adipocyte differentiation pay more attention to microRNA, rather than long non-coding RNAs (lncRNAs).
    OBJECTIVE: To obtain the lncRNAs whose fold change was apparent during adipogenic differentiation, and to further screen the lncRNAs that possibly play a crucial role in adipogenic differentiation for verification.
    METHODS: Subcutaneous fat was obtained from human abdomen. Adipose-derived stem cells were collected using tissue culture method. The third passage of adipose-derived stem cells was used for adipogenic differentiation. Through microarray technology, the expression levels of lncRNAs and mRNA were analyzed at 0, 5 and 12 days in adipogenic differentiation. Combining with bioinformatics report, lncRNAs apparently presented fold change were screened and verified by qRT-PCR.
    RESULTS AND CONCLUSION: Fold change 1.5 (P < 0.05) was considered as a criterion during adipogenic differentiation. The number of up-regulated lncRNAs was 748 for 5 days versus 0 day, 847 for 12 days versus 0 days, 593 for 12 days versus 5 days. At the same time, the down-regulated number was 828 for 5 days versus 0 day, 1 113 for 12 days versus 0 day, 750 for 12 days versus 5 days during adipogenic differentiation. In 
    combination with bioinformatics analysis results, 3 of 28 lncRNAs were related to lipid metabolism: AK304548, BP216319 and DA852857, according to the standard that fold change in 0, 5 and 12 days was higher, and the target genes were known to be associated with adipogenesis-related genes. PCR results showed that the expression of AK304548 and BP216319 and its target gene presented an up-down trend, which is consistent with the microarray sequencing results. These results indicated that lncRNA plays a critical regulatory role in the adipogenic differentiation.


    中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


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    Effects of adipose-derived stem cells cultured with astragaloside on cisplatin-induced apoptosis of renal tubular epithelial cells
    Wang Wei, Jiang Yan, Wang Wei-wei, Zhang Jin-yuan
    2014, 18 (28):  4498-4503.  doi: 10.3969/j.issn.2095-4344.2014.28.012
    Abstract ( 273 )   PDF (421KB) ( 389 )   Save

    BACKGROUND: The validity of stem cell transplantation for acute kidney injury has been verified by many studies. However, the mechanism of repair of tubular epithelial cell injury remains unclear.
    OBJECTIVE: To investigate protective effects and mechanisms of adipose-derived stem cells cultured with astragaloside on the apoptosis of renal tubular epithelial cells induced by cisplatin.
    METHODS: There were four groups. Renal tubular epithelial cells were induced by 2.5 μmol/L cisplatin for 24 hours. Models of renal tubular cell injury were established in the cisplatin group. Adipose-derived stem cells were cocultured with renal tubular epithelial cells of injured kidney in the adipose-derived stem cells + renal tubular epithelial cells group. Using Transwell chamber, adipose-derived stem cells were incubated with 20 mg/L astragaloside for 48 hours, and then cocultured with renal tubular epithelial cells in the astragaloside-incubated adipose-derived stem cells + renal tubular epithelial cells group. Normal renal tubular epithelial cells served as  controls (normal control group).
    RESULTS AND CONCLUSION: Compared with renal tubular epithelial cell injury group, AV/PI and TUNEL results demonstrated that the apoptotic rate and number of renal tubular epithelial cells were apparently reduced in the adipose-derived stem cells + renal tubular epithelial cells group and 20 mg/L astragaloside-incubated adipose-derived stem cells + renal tubular epithelial cells group. ELISA results displayed that insulin-like growth factor-1 levels were significantly elevated in the 20 mg/L astragaloside-incubated adipose-derived stem cells + renal tubular epithelial cells group (P < 0.05). Western blot assay results exhibited that caspase-3 protein levels were noticeably diminished, but Bcl-2 expression was significantly increased in the 20 mg/L astragaloside-incubated adipose-derived stem cells + renal tubular epithelial cells group (P < 0.05). Results suggested that astragaloside-incubated human adipose-derived stem cells suppressed the apoptosis of renal tubular epithelial cells induced by cisplatin, which contributes to the early recovery of injured renal tubule. Its protective mechanism was probably associated with the secretion of insulin-like growth factor-1, inhibition of caspase-3 expression and upregulation of Bcl-2 levels.


    中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


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    Repairing effect of bone marrow mesenchymal stem cells on lung injury in aging rat models
    Wang Zhi-hong, Chen Wei-min, Qu Shuang, Guo Kun-yuan
    2014, 18 (28):  4504-4509.  doi: 10.3969/j.issn.2095-4344.2014.28.013
    Abstract ( 316 )   PDF (562KB) ( 482 )   Save

    BACKGROUND: Studies have shown that exogenous bone marrow mesenchymal stem cells can settle down in lung tissue, participate in long regeneration, but few studies concerned the repair of aging lung injury.
    OBJECTIVE: To observe the effect of bone marrow mesenchymal stem cells on lung injury induced by D-galactose.
    METHODS: A total of 30 Sprague-Dawley rats were equally divided into three groups at random: control group, aging model group and cell treatment group. To establish the aging rats, 10 rats each in the aging model group and cell treatment group were daily subcutaneously injected with D-galactose for 4 months. 3×106 bone marrow mesenchymal stem cells were transplanted via caudal vein in the cell treatment group, once a week, for 4 weeks. Cell medium of equal dose was added in the control and aging model groups. Bone marrow mesenchymal stem cells were transfected by lentiviral vectors expressing green fluorescent protein to determine the implantation of bone marrow mesenchymal stem cells in rat lung. Superoxide dismutase activity and malondialdehyde content in rat lung were measured in each group. The difference in rat lung structure was observed using hematoxylin-eosin staining in each group.
    RESULTS AND CONCLUSION: Bone marrow mesenchymal stem cells marked by green fluorescent protein were implanted in rats, migrated towards lung tissue and survived. Compared with aging model group, superoxide dismutase activity was apparently increased, but malondialdehyde content was obviously diminished in the cell treatment group. In each group, histopathological sections revealed that normal pulmonary alveolus was damaged in the aging model group, showing enlarged air cavity and emphysema. Lung injury was evidently repaired inthe cell treatment group. Results suggested that bone marrow mesenchymal stem cells could repair lung injury in aging rats, and exert anti-aging effects.


    中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


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    Differences between biological characteristics of human periodontal ligament stem cells and human periodontal ligament cells
    Feng Yan, Liang Xue-ping, Zhao Jin, Sun Yu-liang, Zhong Liang-jun
    2014, 18 (28):  4510-4516.  doi: 10.3969/j.issn.2095-4344.2014.28.014
    Abstract ( 443 )   PDF (537KB) ( 617 )   Save

    BACKGROUND: The biological function of human periodontal ligament stem cells is a hot area of research in the treatment of periodontal disease. Human periodontal ligament cells are one of the end cells derived from human periodontal ligament stem cells; meanwhile, it can also provide supports to the development of human periodontal ligament stem cells. However, few studies are reported about the difference of biological characteristics between human periodontal ligament stem cells and human periodontal ligament cells.
    OBJECTIVE: To compare the differences of biological characteristics between human periodontal ligament stem cells and human periodontal ligament cells.
    METHODS: The human periodontal ligament stem cells and human periodontal ligament cells were isolated and purified using tissue explant method and cell clone method, respectively, and then were observed under light microscope to compare the differences of morphology. Cell proliferation curves of human periodontal ligament stem cells and human periodontal ligament cells were drawn respectively with cell counting kit 8 assay. Flow cytometry analysis was used to detect their cell circles and their surface markers expressions. The alkaline phosphatase gene, proliferating cell nuclear antigen gene and Scleraxis gene of human periodontal ligament stem cells and human periodontal ligament cells were detected by Real-time PCR assay. 
    RESULTS AND CONCLUSION: The human periodontal ligament stem cells and human periodontal ligament cells showed a notable difference in morphology under the light microscope observation. During the first 5 days, the cell proliferation curve of human periodontal ligament stem cells was lower than that of human periodontal ligament cells, but 5 days later, the curve of human periodontal ligament stem cells was significantly higher than that of human periodontal ligament cells. The cell circles of human periodontal ligament stem cells and human periodontal ligament cells were 41.1% and 23.9%, respectively. The surface markers of human periodontal ligament stem cells and human periodontal ligament cells were similar, but their expression rates had significant difference. The expressions of alkaline phosphatase gene, proliferating cell nuclear antigen gene and Scleraxis gene of human periodontal ligament stem cells were significantly higher than those of human periodontal ligament cells. The above results suggest that human periodontal ligament stem cells have much stronger potential ability than human periodontal ligament cells in osteogensis and cell proliferation.


    中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


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    Optimal concentration of modified platelet-rich plasma to promote the proliferation of dental pulp stem cells from deciduous teeth
    Wen Jun, Wu Bu-ling, Duan Jian-min, Li Hong-tao, Li Si-han, Li Xin
    2014, 18 (28):  4517-4523.  doi: 10.3969/j.issn.2095-4344.2014.28.015
    Abstract ( 395 )   PDF (497KB) ( 414 )   Save

    BACKGROUND: Previous experiments have shown that modified platelet-rich plasma activated by liquid nitrogen freezing and thawing can promote the proliferation of human bone marrow mesenchymal stem cells and dental pulp stem cells in a concentration-dependent manner.
    OBJECTIVE: To investigate the effect of modified platelet-rich plasma at different concentrations on the proliferation of dental pulp stem cells from human exfoliated deciduous teeth.
    METHODS: Platelets were selected and harvested by automatic blood cell analyzer, and then activated by liquid nitrogen freezing and thawing. α-MEM served as basal medium. Different concentrations of modified platelet-rich plasma (2%, 5%, 10%, 20%) or 10% fetal bovine serum were added, respectively. The difference in cell proliferation was observed.
    RESULTS AND CONCLUSION: Modified platelet-rich plasma at different concentrations could promote the proliferation of dental pulp stem cells from deciduous teeth. The effects of 2% modified platelet-rich plasma and 10% fetal bovine serum on promoting the proliferation of dental pulp stem cells from deciduous teeth were similar. These results indicated that 2% modified platelet-rich plasma could promote the proliferation of dental pulp stem cells from deciduous teeth, and substitute for fetal bovine serum in the amplification of dental pulp stem cells from deciduous teeth in vitro.


    中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


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    Mental health status of relative donors versus unrelated donors before and after hematopoietic stem cell collection
    Hou Yan-hong, Zhao Shu-yan, Qi Qinjiazi, Liu Li-hui, Chen Xiao-fei, Shi Bing, Zhu Ling, Xu Chen, Jia Rui-ming, Wang Wei-wei, Yang Jing, Liang Yong-qing, Tan Yi, Li Fu-xing, Ye Li-ping
    2014, 18 (28):  4524-4529.  doi: 10.3969/j.issn.2095-4344.2014.28.016
    Abstract ( 423 )   PDF (316KB) ( 408 )   Save

    BACKGROUND: Currently, hematopoietic stem cell transplantation mainly depends on unrelated donors. Mental state of the unrelated donors is very important to ensure the successful cell transplantation.
    OBJECTIVE: To compare mental and physical health status of relative and unrelated donors during the hematopoietic stem cell collection.
    METHODS: We compared the mental (Symptom Checklist-90) and physical (temperature, breath, pulse, and blood pressure) health status of relative and unrelated donors at admission, 1 day before cell collection, and 1-2 days after cell collection.
    RESULTS AND CONCLUSION: At admission, there was no difference in the mental health status of relative and unrelated donors (P > 0.05), while the scores on Symptom Checklist-90 were significantly higher in the unrelated donors than the relative donors, including total score, forced, depression, anxiety, hostility, and fear (P < 0.05). The physical signs were steady in the unrelated and relative donors, but the difference in breath and systolic blood pressure was of great significance before and after cell collection in the two groups. These findings indicate that during cell collection, the unrelated donors exhibit heavier mental load than the relative donors, and psychological counseling and health guidance are necessary.


    中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


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    Recombinant human erythropoietin combined with bone marrow mesenchymal stem cell transplantation for myocardial damage in sepsis rats
    Teng Jin-long, Li Dan, Yu Hai-chu, Cai Shang-lang, Pan Xin-ting
    2014, 18 (28):  4530-4534.  doi: 10.3969/j.issn.2095-4344.2014.28.017
    Abstract ( 219 )   PDF (485KB) ( 327 )   Save

    BACKGROUND: Erythropoietin and bone marrow mesenchymal stem cells have been shown to affect myocardial apoptosis. However, few studies concerned their combined application to sepsis-related myocardial injury.
    OBJECTIVE: To observe the effects of the combination of erythropoietin and bone marrow mesenchymal stem cells on pathology and apoptosis of sepsis rat cardiomyocytes.
    METHODS: A total of 50 Sprague-Dawley rats were randomly selected and assigned to five groups (n=10). Sepsis models were established by cecal ligation perforation method. Rat models in the bone marrow mesenchymal stem cell group, erythropoietin group and erythropoietin + bone marrow mesenchymal stem cell group were respectively treated with bone marrow mesenchymal stem cells, erythropoietin and erythropoietin + 
    bone marrow mesenchymal stem cells immediately after model induction via intraperitoneal injection or caudal vein. Model group received cecum ligation and puncture. Control group did not undergo any treatment after the abdomen was opened. Model and control groups were infused with an equal volume of physiological saline via caudal vein. At 24 hours, experimental animals were sacrificed by anesthesia. Myocardial specimens were collected. Myocardial appearance was observed using hematoxylin-eosin staining. Bax, Caspase-3 and Bcl-2 were tested by western blot assay.
    RESULTS AND CONCLUSION: Hematoxylin-eosin staining: cardiomyocytes were regularly arranged, showing integrated structure in the control group. Extensive myocardial fiber breakage, disordered arrangement, cardiomyocyte swelling or shrinkage, and vacuolar degeneration were observed in the model group. Moreover, myocardial interstitial vascular congestion, edema, and inflammatory cell infiltration were visible. Myocardial tissue was similar between erythropoietin and bone marrow mesenchymal stem cell groups, with the presence of mild inflammatory cell infiltration and scattered normal cardiomyocytes. In the erythropoietin + bone marrow mesenchymal stem cell group, cardiomyocytes were slightly damaged. Interstitial vascular congestion was not apparent, and a few inflammatory cells infiltrated. Western blot assay results demonstrated that Bcl-2 protein expression was significantly higher (P < 0.01), but Bax and Caspase-3 protein expression was lower (P < 0.05) in the erythropoietin + bone marrow mesenchymal stem cell group than in the erythropoietin, model and control groups. The combination of erythropoietin and bone marrow mesenchymal stem cells in treatment of sepsis-related myocardial injury could lessen myocardial pathological changes, and inhibit cardiomyocyte apoptosis. The mechanisms maybe exert by upregulating anti-apoptotic protein expression and downregulating apoptotic protein expression.


    中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


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    Isolation, culture and identification of adipose-derived stem cells from mouse epididymis 
    Zhang Jian-qing, Ji Jia-lin, Cui Xin-ming, Zhang Qi, Li Yan-ru
    2014, 18 (28):  4535-4541.  doi: 10.3969/j.issn.2095-4344.2014.28.018
    Abstract ( 763 )   PDF (1062KB) ( 1658 )   Save

    BACKGROUND: As a new kind of adult stem cells, adipose-derived stem cells get more and more attention, because of rich source, drawing materials easily and powerful proliferation. 
    OBJECTIVE: To isolate and culture adipose-derived stem cells from the epididymal adipose tissue in mice, and to identify their biological characteristics.
    METHODS: Adipose tissue was obtained from epididymis in mice by aseptically cutting. The tissue was digested using collagenase. Adipose-derived stem cells were separated and purified by using one digestion, multiple collection method and differential adhesion method. The morphology of adipose-derived stem cells was observed using inverted microscopy and transmission electron microscopy. Growth curve of adipose-derived stem cells was drawn. Immunophenotype of adipose-derived stem cells was identified by flow cytometry. Adipose-derived stem cells were induced to differentiate into adipocytes and osteocytes using cell inductors. Compatibility of adipose-derived stem cells and collagen scaffold material was observed using scanning electron microscope.  
    RESULTS AND CONCLUSION: Adipose-derived stem cells exhibited long spindle-like or fibroblast-like appearance, grew intensively and arranged in scroll and fascicular shape. In vitro, adipose-derived stem cells could be passaged to passage 9 under the inverted microscope. Under the transmission electron microscope, adipose-derived stem cells showed abundant microvilli on the cell surface. The nuclei were big in size. Some organelles were seen in cytoplasma, such as mitochondria and rough endoplasmic reticulum. Adipose-derived stem cells expressed CD44 and CD29, did not express CD34. After inducing by inductor, many small lipid droplets were seen in the cytoplasm of adipose-derived stem cells. The small lipid droplets were dyed red with oil red O. After induction of osteogenic inductor, the boundary line among adipose-derived stem cells was not clear and the structure of cells was fuzzy in the growth-intensive areas. There were many strong refractive granular material deposits at that field after dyeing with alizarin red. Scanning electron microscope revealed that adipose-derived stem cells were spread on the collagen scaffold. Results suggested that adipose-derived stem cells isolated by this method could amplify in vitro and stably subcultured. Under a certain inducing condition, adipose-derived stem cells could differentiate into osteoblasts and adipocytes, which showed a good compatibility with collagen scaffold.


    中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


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    Effects of transforming growth factor beta 3 on the proliferation and mineralization of dental pulp stem cells from human deciduous teeth
    Ren Fei, Liu Jian-ping, Lin Jin-mei, Zhou Hui, Chen Xiao-chun, Xu Ping-ping, Yang Qin
    2014, 18 (28):  4542-4548.  doi: 10.3969/j.issn.2095-4344.2014.28.019
    Abstract ( 312 )   PDF (567KB) ( 401 )   Save

    BACKGROUND: The role of transforming growth factor β superfamily has been reported in bone mineralization of various types of stem cells, but the effects of transforming growth factor β3 (TGF-β3) combined with heparin on proliferation and mineralization of dental pulp stem cells from human deciduous teeth remains to be studied.
    OBJECTIVE: To evaluate the effects of TGF-β3 on the proliferation and mineralization of dental pulp stem cells from human deciduous teeth. 
    METHODS: The enzyme digestion method was utilized to separately culture dental pulp stem cells from human deciduous teeth. The cell colony forming efficiency was determined. Flow cytometry was utilized to identify cell surface marker CD146. Immunochemistry for Vimentin and STRO1 was performed to measure dental pulp stem cells from human deciduous teeth. The third passage dental pulp stem cells from human deciduous teeth cultured in vitro were intervened with heparin and TGF-β3 of 1, 5, 25 μg/L mass concentration. The MTS method was applied to measure cell growth curves. Alizarin red staining was carried out. The changes in alkaline phosphatase activity were determined with alkaline phosphatase kit.
    RESULTS AND CONCLUSION: The cell colony forming efficiency was high. Cells were positive for CD146, and strongly positive for Vimentin and STRO1. Dental pulp stem cells from human deciduous teeth were identified. MTS assay indicated that there was no obvious effect on promoting proliferation of dental pulp stem cells from human deciduous teeth after stimulation of TGF-β3. Detection results of alkaline phosphatase activity demonstrated that the combination of TGF-β3 and heparin could strengthen the alkaline phosphatase activity of dental pulp stem cells from human deciduous teeth with increased concentration. Alkaline phosphatase activities were significantly higher in the TGF-β3 + heparin group, TGF-β3 group and heparin group than in the control group (P < 0.01). Alizarin red staining was positive in the TGF-β3 + heparin group, and the staining was strongest in the 5 μg/L TGF-β3 + heparin group. Results indicated that TGF-β3 combined with heparin promoted mineralization of dental pulp stem cells from human deciduous teeth.


    中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


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    Liver cancer stem cell isolation and biological characteristics
    Liu Yan, Zhou Yue-su, He Jing, Liu Peng
    2014, 18 (28):  4549-4554.  doi: 10.3969/j.issn.2095-4344.2014.28.020
    Abstract ( 466 )   PDF (359KB) ( 399 )   Save

    BACKGROUND: Normal stem cells and tumor stem cells have differences in cell signaling pathway of gene expression and dependence. How to find a therapeutic method of selectively killing tumor stem cells is a topic that should be studied greatly.
    OBJECTIVE: To isolate human liver cancer stem cells MHCC97 and to investigate the biological characteristics of them.
    METHODS: Tumor stem cells were selected from highly metastatic human hepatoma cell line MHCC97 by flow cytometry. CD133-CD34- MHCC97 from normal human liver stem cells and CD133+CD34+ MHCC97 from tumor stem cells were isolated. Their phenotypes, growth curve, cell cycle and multi-lineage differentiation were measured.
    RESULTS AND CONCLUSION: Phenotypes of CD133+CD34+ MHCC97 cancer stem cells were CD133+ and CD34+ and they had the same growth curve and cell cycle with the liver stem cells CD133-CD34- MHCC97, besides, they had the differential ability to epithelial cells and endothelial cells that proved to be stem cells. These results indicated that human cancer stem cells CD133+CD34+ MHCC97 had the specific characteristics of tumor stem cells, could differentiate into epithelial cells and endothelial cells, had the biological property of tumor stem cells, was an origin of tumor relapse and metastasis, and a target of clinical therapy.


    中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


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    Screening main genes during mesenchymal stem cell transplantation in repair of inflammatory bowel tissue
    Cao Yan-wen, Wei Ya-ming, Li Yu-yuan, Nie Yu-qiang, Wu Qian
    2014, 18 (28):  4555-4562.  doi: 10.3969/j.issn.2095-4344.2014.28.021
    Abstract ( 255 )   PDF (781KB) ( 343 )   Save

    BACKGROUND: Previous studies have verified that mesenchymal stem cells could be transplanted into inflammatory bowel mucosa to repair inflammatory bowel tissue.
    OBJECTIVE: To observe the differential gene expression in large intestine before and after mesenchymal stem cell transplantation in repair of inflammatory bowel tissue of rats using microarray technology, and to primarily discover the main genes during mesenchymal stem cell transplantation, differentiation, and reparation in inflammatory colorectal tissue region.
    METHODS: Healthy Sprague-Dawley rats were randomly divided into two groups. Experimental rat models of inflammatory bowel disease were established using trinitrobenzene sulfonic acid via enema. At 24 hours after model establishment, green fluorescent protein-labeled mesenchymal stem cells were infused via the caudal vein. The control group was treated with physiological saline by enema, instead of trinitrobenzene sulfonic acid. At 28 days, large intestine was obtained from the experimental group and control group. Differentially expressed genes were screened in the experimental and control groups using microarray technique.
    RESULTS AND CONCLUSION: The microarray analysis results showed that there were 388 differential genes in the control and experimental groups (P < 0.05, FC > 2), in which 191 were up-expressed, and 197 were down-expressed. All of these genes were mainly involved in inflammatory reaction, immune reaction and cell differentiation. In the top 10 up-regulation and down-regulation differential genes (totally 20 genes), 3 genes were involved in inflammation, 3 genes were involved in immune reaction, and 2 genes were related to stem cell differentiation. In the 388 genes, 33 were related to signaling pathways (P < 0.05), 6 related to inflammation, 8 related to immunity, and 5 related to stem cell differentiation. Results suggested that the main genes involved in mesenchymal stem cells in repair of inflammatory bowel tissue were primarily screened using gene expression microarray technique.


    中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


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    Human cytomegalovirus detection in hematopoietic stem cell transplantation: value of fluorescence quantitative PCR in the early diagnosis
    Liu Chuan, Zou Ye-qing, Shi Qing-zhi
    2014, 18 (28):  4563-4567.  doi: 10.3969/j.issn.2095-4344.2014.28.022
    Abstract ( 303 )   PDF (289KB) ( 430 )   Save

    BACKGROUND: To improve the survival rate of transplanted hematopoietic stem cells, dynamic monitoring of plasma content of human cytomegalovirus (HCMV) and rapid screening of early active HCMV infection in hematopoietic stem cell tranplantation recipients, thus to guide the clinical medication, is preferred.
    OBJECTIVE: To evaluate the usefulness of fluorescence quantitative PCR assay for early detection of HCMV activation after hematopoietic stem cell transplantation.
    METHODS: Real-time fluorescence quantitative PCR assay was applied for HCMV monitoring in 656 blood samples from 41 hematopoietic stem cell tranplantation recipients and 60 control blood samples.
    RESULTS AND CONCLUSION: In 656 blood samples, 96 samples were positive, and the HCMV DNA copies ranged from 5.0×102 to 1.0×107 IU/mL. Timely initiation of therapy resulted in the rapid clearance of DNA-viraemia but it recurrenced in short time by drug-resistent. Two cases from 12 postive recipients died from HCMV infection. The quantitative detection of HCMV DNA by real-time PCR is a rapid method for monitoring HCMV infection and the early diagnosis in patients after hematopoietic stem cell transplantation.


    中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


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    Lung cancer stem cells and lung cancer
    Liu Zhe-liang, Xiao Gao-ming, Chen Yue-jun, Wu Guan-yu
    2014, 18 (28):  4568-4572.  doi: 10.3969/j.issn.2095-4344.2014.28.023
    Abstract ( 646 )   PDF (269KB) ( 865 )   Save

    BACKGROUND: Lung cancers are highly heterogeneous and resistant to available therapeutic agents, with a five year survival rate of less than 15%. It has been difficult to determine the basis of lung cancer heterogeneity and drug resistance. Cancer stem cell model has attracted a significant amount of attention in recent years as a viable explanation for the heterogeneity, drug resistance, dormancy and recurrence and metastasis of various tumors.
    OBJECTIVE: To summarize the current understanding of lung cancer stem cells, including their histological types and tumor growth areas, and to discusses the prognosis of lung cancer and its relationship with lung cancer stem cells, in an effort to eradicate these cells to combat lung cancer.
    METHODS: In order to search relevant articles about the lung cancer stem cell and its relationship with lung cancer from PubMed and Sciencedirect databases (from 1990 to 2014), a computer-based search was performed, using the key words of “lung cancer, cancer stem cell, lung cancer stem cell, lung cancer occur, tumor heterogeneity, drug resistance, gene mutation, signal pathways” in English. After eliminating literatures which were irrelevant to research purpose or containing a similar content, 48 articles were chosen for further analysis.
    RESULTS AND CONCLUSION: The cancer stem cell model has gained considerable support recently in context of lung cancers and stem-like cells that are associated with aggressive cancer behavior, metastatic progression, resistance to therapy and relapse. Since lung cancer stem cells are thought to consist of a heterogeneous population depending on the histology and site of tumors, and multiple signaling pathways might have to be targeted to effectively eliminate lung cancer stem cells for therapeutic benefit. It can be imagined that the multidisciplinary efforts currently under way to characterize and target stem-like cells in lung cancer will reap significant therapeutic benefits in the future.


    中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


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    Surface markers and skin differentiation of epidermal stem cells: theoretical features and clinical feasibility
    Liu Guang-rong, Chen Qing-yuan, Hu Guan-song, Cai Yu-chun
    2014, 18 (28):  4573-4577.  doi: 10.3969/j.issn.2095-4344.2014.28.024
    Abstract ( 414 )   PDF (359KB) ( 1107 )   Save

    BACKGROUND: Epidermal stem cells as special stem cells of the skin not only plays an important role in maintaining the metabolism of skin, but also is closely related to wound repair, which are the basis for the occurrence and repair of skin and its appendages. Now, epidermal stem cells have been paid great attention on the research of gene therapy and cell therapy with its specific biological advantages.
    OBJECTIVE: To summarize the research status on features and clinical application of epidermal stem cells.
    METHODS: A computer-based online retrieval of CNKI, PubMed database and Google scholar was performed for searching papers about clinical application of epidermal stem cells using the keywords of “epidermal stem cells, stem cells division, stem cells culture, clinical application” in Chinese and English. Older theoretical perspectives and repetitive research were excluded. Finally, only 41 articles were included in result analysis.
    RESULTS AND CONCLUSION: Epidermal stem cells have the great potential of proliferation and multi-directional differentiation, and have important significances for large-area skin defects (burn and trauma), skin tissue engineering, and gene therapy. There are still many problems that need to be solved, such as how to screen surface markers special for epidermal stem cells and how to induce skin differentiation of epidermal stem cells.


    中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


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    Differentiation potential of bone marrow mesenchymal stem cells into hepatocyte-like cells
    Fan Jing-jing
    2014, 18 (28):  4578-4582.  doi: 10.3969/j.issn.2095-4344.2014.28.025
    Abstract ( 273 )   PDF (341KB) ( 429 )   Save

    BACKGROUND: Studies have demonstrated that bone marrow mesenchymal stem cells can be induced to differentiate into hepatocyte-like cells under certain condition, which provides a new idea for the treatment of end-sate liver diseases, such as acute hepatic failure.
    OBJECTIVE: To review the discovery, isolation and culture, induction and differentiation, and application prospects of bone marrow mesenchymal stem cells.
    METHODS: A computer-based online research of CNKI and PubMed databases was performed to collect articles about bone marrow mesenchymal stem cells and their differentiation into hepatocyte-like cells published between 1999 and 2014. The key words were “hepatocyte-like cells, bone marrow mesenchymal stem cells, cell differentiation” in Chinese and English. Finally, 52 articles were included in result analysis.
    RESULTS AND CONCLUSION: Currently, the most important issue in liver tissue engineering is to find a kind of seed cells with stable traits and liver-specific functions. Mature hepatocytes are difficult to be harvested, and immune rejection and difficulty in in vitro culture also severely limit the development of liver transplantation. Bone marrow mesenchymal stem cells characterized as multiple differentiation, rapid self-renewal, easy to be cultured and proliferated have been considered as the most promising source of cells. Increasing studies have shown that bone marrow mesenchymal stem cells can differentiate into liver cells in vitro and in vivo, and differentiated cells have the synthesis and secretion functions same as hepatocytes. But how to proliferate such a large number of cells, to maintain the good differentiation potential of cells, to optimize the culture conditions in vitro as well as mechanisms of induction and differentiation mechanisms and clinical security need further studies.


    中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


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    In vitro expansion of human mesenchymal stem cells in animal serum-free media
    Wu Di, Wu Xiao-yun, Wu Yan
    2014, 18 (28):  4583-4587.  doi: 10.3969/j.issn.2095-4344.2014.28.026
    Abstract ( 410 )   PDF (323KB) ( 582 )   Save

    BACKGROUND: Mesenchymal stem cells have been an important source of seed cells for tissue engineering, regenerative medicine and immunosuppressive therapy areas. The large expansion is the key to the clinical application of mesenchymal stem cells.
    OBJECTIVE: To summarize the research progress in the in vitro expansion of human mesenchymal stem cells in animal serum-free media.
    METHODS: A computer-based online retrieval of CNKI and PubMed databases was performed to search papers published between 2004 and 2014 using the key words of “mesenchymal (stromal) stem cells, animal serum-free media, humanized media, human serum, umbilical cord blood serum, platelet rich plasma, platelet lysate, defined medium” in English and Chinese, respectively. Finally, 41 articles were retained.
    RESULTS AND CONCLUSION: Human serum, umbilical cord blood serum, platelet rich plasma, platelet lysate and defined medium can replace fetal bovine serum for large-scale expansion of mesenchymal stem cells in vitro. These media or additives have their advantages and disadvantages; therefore, it is urgent to guarantee the functional effectiveness and transplantation safety before large-scale, systematic clinical application of mesenchymal stem cells.


    中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


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    Safety analysis of umbilical cord blood stem cell transplantation combined with nerve growth factor in the treatment of cerebral palsy in children
    Li Ying
    2014, 18 (28):  4588-4592.  doi: 10.3969/j.issn.2095-4344.2014.28.027
    Abstract ( 274 )   PDF (314KB) ( 420 )   Save

    BACKGROUND: Umbilical cord blood stem cell transplantation combined with nerve growth factor in the treatment of children with cerebral palsy is a clinical hotspot.
    OBJECTIVE: To analyze the efficacy and safety of umbilical cord blood stem cell transplantation combined with nerve growth factor in the treatment of cerebral palsy in children.
    METHODS: A total of 80 children with cerebral palsy admitted at the Department of Pediatrics, Hongqiao Hospital of Tianjin, China from 2011 November to 2013 February were enrolled, and according to therapeutic methods, they were divided into experimental group (n=40; treated with umbilical cord blood stem cell transplantation combined with nerve growth factor) and control group (n=40; treated with nerve growth factor alone).
    RESULTS AND CONCLUSION: After treatment, scores on comprehensive function assessment scale and gross motor function measure scale were significantly increased in the two groups (P < 0.05), while white cell count, neutrophil fraction, and levels of total bilirubin, alanine aminotransferase and aspartate aminotransferase were also increased (P < 0.05). Moreover, the experimental group showed a better outcome than the control group after treatment (P < 0.05). All of the patients had no serious adverse reactions. Umbilical cord blood stem cell transplantation combined with nerve growth factor has dramatic curative effects in children with severe cerebral palsy significant curative effect, with high safety.


    中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


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