Differences between biological characteristics of human periodontal ligament stem cells and human periodontal ligament cells

doi:10.3969/j.issn.2095-4344.2014.28.014

Abstract

Abstract:

BACKGROUND: The biological function of human periodontal ligament stem cells is a hot area of research in the treatment of periodontal disease. Human periodontal ligament cells are one of the end cells derived from human periodontal ligament stem cells; meanwhile, it can also provide supports to the development of human periodontal ligament stem cells. However, few studies are reported about the difference of biological characteristics between human periodontal ligament stem cells and human periodontal ligament cells.
OBJECTIVE: To compare the differences of biological characteristics between human periodontal ligament stem cells and human periodontal ligament cells.
METHODS: The human periodontal ligament stem cells and human periodontal ligament cells were isolated and purified using tissue explant method and cell clone method, respectively, and then were observed under light microscope to compare the differences of morphology. Cell proliferation curves of human periodontal ligament stem cells and human periodontal ligament cells were drawn respectively with cell counting kit 8 assay. Flow cytometry analysis was used to detect their cell circles and their surface markers expressions. The alkaline phosphatase gene, proliferating cell nuclear antigen gene and Scleraxis gene of human periodontal ligament stem cells and human periodontal ligament cells were detected by Real-time PCR assay.
RESULTS AND CONCLUSION: The human periodontal ligament stem cells and human periodontal ligament cells showed a notable difference in morphology under the light microscope observation. During the first 5 days, the cell proliferation curve of human periodontal ligament stem cells was lower than that of human periodontal ligament cells, but 5 days later, the curve of human periodontal ligament stem cells was significantly higher than that of human periodontal ligament cells. The cell circles of human periodontal ligament stem cells and human periodontal ligament cells were 41.1% and 23.9%, respectively. The surface markers of human periodontal ligament stem cells and human periodontal ligament cells were similar, but their expression rates had significant difference. The expressions of alkaline phosphatase gene, proliferating cell nuclear antigen gene and Scleraxis gene of human periodontal ligament stem cells were significantly higher than those of human periodontal ligament cells. The above results suggest that human periodontal ligament stem cells have much stronger potential ability than human periodontal ligament cells in osteogensis and cell proliferation.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: periodontal ligament, stem cells, cell proliferation, cell differentiation

CLC Number:

R394.2

Feng Yan, Liang Xue-ping, Zhao Jin, Sun Yu-liang, Zhong Liang-jun. Differences between biological characteristics of human periodontal ligament stem cells and human periodontal ligament cells[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(28): 4510-4516.

Figures/Tables 7

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