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09 July 2014, Volume 18 Issue 29 Previous Issue    Next Issue

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Effect of lipoxin receptor agonist on human cytomegalovirus replication and proliferation Chen Xiao-hong, Shu Sai-nan, Liu Xing-lou, Wang Hui, Zhang Ju, Du Xiao-yi, Li Ge, Fang Feng 2014, 18 (29):  4593-4598.  doi: 10.3969/j.issn.2095-4344.2014.29.001 Abstract ( 340 )   PDF (628KB) ( 332 )   Save BACKGROUND: The anti-inflammation and protective effects of lipoxin have been verified in several immunity-related disease models. Preliminary studies of our research group have shown that, lipoxin receptor agonist BML-111 has negative regulation effects on the human cytomegalovirus (HCMV)-induced immunological injury. However, the effect of BML-111 on the HCMV replication remains unclear. OBJECTIVE: To observe the influence of lipoxin receptor agonist BML-111 on HCMV replication and proliferation in THP-1 macrophages and human embryonic lung fibroblasts. METHODS: THP-1 macrophages were infected by HCMV AD169 strain, and were divided into three groups: mock infection, HCMV infection, HCMV+BML-111. The final concentration of BML-111 was 100 nmol/L. Cells in each group were collected at 0, 1, 2, 4, 12, 36, 48 hours, the mRNA levels of IE86 and pp65 in the THP-1 macrophages were tested by RT-PCR method. Human embryonic lung fibroblasts were infected with HCMV (MOI=0.1), and were divided into two groups: HCMV infection and HCMV+BML-111. The patho-morphous changes of human embryonic lung fibroblasts were observed under light microscope, and the cell number was measured. The infective virus titer changes in human embryonic lung fibroblasts were examined by plaque assay. RESULTS AND CONCLUSION: After the macrophages were infected by HCMV, compared with the mock infection group, the mRNA levels of IE86 and pp65 in the HCMV group and HCMV+BML-111 group were increased significantly; compared with the HCMV infection group, the mRNA levels of IE86 and pp65 in the HCMV+BML-111 group were increased significantly in the early stage (within 4 hours) after infection, but the pp65 mRNA levels were decreased significantly in the medium and late stages (24-72 hours) after infection. After human embryonic lung fibroblasts were infected by HCMV, the degree of the patho-morphous in the HCMV+BML-111 group reached 100% 2 days earlier than the of HCMV infection group. The infective virus titer reached the peak 2 days earlier than the HCMV infection group, but no significant difference was found between the two groups. BML-111 accelerates the replication of HCMV in the early stage of infection, but inhibits the expression of pp65 gene in the late stage. BML-111 has no impact on the proliferation of the infective HCMV titer in vitro. 中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 全文链接： Figures and Tables | References | Related Articles | Metrics

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Database construction and data analysis of von Willebrand factor and platelet glycoprotein Ib alpha mutations Li Na, Li Qu-huan, Wang An-qi 2014, 18 (29):  4599-4604.  doi: 10.3969/j.issn.2095-4344.2014.29.002 Abstract ( 289 )   PDF (768KB) ( 423 )   Save BACKGROUND: The interaction between von Willebrand Factor (VWF) and platelet surface receptor, platelet glycoprotein Ib alpha (GPIbα), plays a key role in the process of platelet adhesion, spread and aggregation. Currently, the database collecting VWF mutations is not complete and GPIbα database has not been constructed yet. OBJECTIVE: The construction of VWF and GPIbα mutation database can help the researchers in this field to find the important mutation information of these two molecules quickly. METHODS: For constructing VWF and GPIbα mutation database, data were collected from Uniprot, VWFdb and literatures by keywords search, the dynamics website was designed using MySQL+Apache+PHP combination. RESULTS AND CONCLUSION: The database contains 341, 13 and 3 920 wild and mutation records of VWF, GPIbα, and A1 domain. The website connecting with backend database is comprised of the background introduction, the pathological pictures and data download page and so on. Especially, the information of mutations and virtual mutations can be searched and analyzed through search engine with sample and logical strategy. This database will be greatly beneficial to study VWF and GPIbα. Furthermore, it can offer some new therapeutic approaches to inhibit the binding of VWF and GPIbα, which serve as an attractive drug target in von Willebrand Disease. 中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 全文链接： Figures and Tables | References | Related Articles | Metrics

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Designation and silencing function of the small interfering RNA of HLA-A2 Liu Jian-sheng, Zhao Gang, Pei Dan, Zheng De-yu 2014, 18 (29):  4605-4610.  doi: 10.3969/j.issn.2095-4344.2014.29.003 Abstract ( 391 )   PDF (655KB) ( 387 )   Save BACKGROUND: Human leukocyte antigen (HLA), the major histocompatibility complex of human, plays an important function in the transplant rejection. Decreasing the expression of HLA will prolong the survival time of transplants. OBJECTIVE: To design small interfering RNA (siRNA) of HLA-A2 and to detect the effect of siRNA-HLA-A2 on the expression of HLA-A2. METHODS: Four kinds of siRNA-HLA-A2 domains were designed, and recombinant lentivirus expression vector were formed. The 293T cells, highly expressing HLA-A2, were infected in vitro. Then the knockout efficacy of four domains was detected to select the highly efficient siRNA-HLA-A2 target sequences. The human embryo lung fibroblasts were cultured in vitro and infected with the lentivirus carrying the target sequence. The infecting efficiency of LV-siRNA-HLA-A2 was observed under the fluorescence microscope and the silence function of this siRNA in human embryo lung fibroblasts was detected by western blot analysis. RESULTS AND CONCLUSION: According to the mRNA sequence of HLA-A2 in Genbank, three siRNAs were designed and synthesized. In vitro, the over expression of HLA-A2 in 293K cells was successfully silenced. The HLA-A2 expression in human embryo lung fibroblasts was also efficiently silenced after the human embryo lung fibroblasts were infected by the highly efficient siRNA of HLA-A2. The efficacy was up to 80%. 中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 全文链接： Figures and Tables | References | Related Articles | Metrics

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Significance of young females’ different vertical facial types on the aesthetic evaluation of facial profiles Wang Yue, Lei Yong-hua, Yue Li, Peng Yi 2014, 18 (29):  4611-4617.  doi: 10.3969/j.issn.2095-4344.2014.29.004 Abstract ( 558 )   PDF (816KB) ( 484 )   Save  BACKGROUND: It is confirmed that the characteristics of soft and hard tissue development vary largely in different vertical facial types, which reflect the development trend of the patients and the controlling degree on anchorage. OBJECTIVE: To evaluate the influence of different vertical facial types on the aesthetic evaluation of facial profiles in young females, and analyze the perception of facial attractiveness from different people. METHODS: Three beautiful females, with high, average and low mandibular angle, respectively were selected according to the measurement data of projection and the orthodontist’s aesthetic appreciation. They were yellow race, bilateral facial appearance was basically symmetrical, and no other maxillofacial disease was found. They also received no orthodontic or orthognathic treatment. The profile digital photographs and cephalograms of three females were collected. The positions of soft tissue lip and soft tissue chin were altered incrementally with Dolphin Imaging and Management software. Twenty-five new pictures were generated by the small scale (2 mm per unit) modification in the sagittal direction. By using “acceptable facial appearance” as a filter, 15 orthodontists selected 11 from 25 pictures. Fifty parents of orthodontic patients, 50 orthodontic patients, and 50 amateurs were taken as the evaluators for subjective aesthetic ratings and for the assessment of the influence of vertical facial types on the facial profile attractiveness. RESULTS AND CONCLUSION: With low and average angle, straight profile, slightly protrude chin was perceived to be beautiful. With high angle, straight profile, slightly protrude profile was perceived to be beautiful, and evaluation results are consistent between men and women. Evaluators are in agreement when it comes to evaluating the perception of facial attractiveness of three different vertical facial types profile in young females. They also have certain difference in aesthetic tendencies, but when it comes to straight profile they share the same preference. Although different vertical facial types profile has an impact on the profile of lip and chin, but it can’t represent the aesthetic preference of all evaluators. 中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 全文链接： Figures and Tables | References | Related Articles | Metrics

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Expression of bone morphogenetic protein 7, Gremlin, vascular endothelial growth factor and high mobility group box-1 in keloid and normal skin Liu Yu-fang, Lin Mao, Hou Bin-bin, Hou Na, Liu Xia, Wang Dan, Xu Xue-zhu 2014, 18 (29):  4618-4624.  doi: 10.3969/j.issn.2095-4344.2014.29.005 Abstract ( 420 )   PDF (2440KB) ( 376 )   Save BACKGROUND: The development of keloid is a progress of fibrosis in wound healing, and involves various fibrosis-related cytokines. Bone morphogenetic protein 7 (BMP7), Gremlin and high mobility group box-1 (HMGB1) play an important role in fibrosis of many organs, but their role in keloid tissue has rarely been reported. OBJECTIVE: To investigate the role of BMP7, Gremlin, vascular endothelial growth factor (VEGF) and HMGB1 in the development of keloid. METHODS: The protein levels and distribution of BMP7, Gremlin, VEGF and HMGB1 in 20 cases of keloid and 20 cases of normal skin were detected by immunohistochemistry and western blot analysis, respectively. And the correlations among expression levels of BMP7, Gremlin, VEGF and HMGB1 in keloid were analyzed. RESULTS AND CONCLUSION: In keloid tissue, the expression levels of Gremlin, VEGF and HMGB1 were significantly higher than that in normal skin (P < 0.01), while the expression levels of BMP7 were significantly lower (P < 0.01). The levels of Gremlin were negatively correlated with the levels of BMP7 (r=-0.539, P < 0.05). And the levels of VEGF were positively correlated with the levels of HMGB1 (r=0.56, P < 0.05). The overexpression of Gremlin and decreased expression of BMP7, as well as the increased expression of HMGB1 and VEGF, may contribute to the pathogenesis of fibrosis in the development of keloid. 中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 全文链接： Figures and Tables | References | Related Articles | Metrics

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Differentiation of HaCaT cells infected with lentivirus Hou Na, Hou Bin-bin, Wang Xiu-li, Liu Yu-fang, Guo Xin, Lin Mao, Xu Xue-zhu 2014, 18 (29):  4625-4629.  doi: 10.3969/j.issn.2095-4344.2014.29.006 Abstract ( 436 )   PDF (1519KB) ( 596 )   Save BACKGROUND: YY1 is mainly expressed in the undifferentiated epidermic cells in mouse basal lamina, and the expression level is gradually down-regulated as the differentiation towards suprabasal lamina. The differential expression indicates that, YY1 is one of the regulators in the process of epidermic cells differentiation. OBJECTIVE: To observe the effects of YY1 over-expression on the differentiation of HaCaT cells infected with lentivirus. METHODS: Lentivirus-YY1 was transferred into the HaCaT cells by using Lipofectamine 2000. After selection of the puromycin, monoclonal cell lines were established, and the control group were lentivirus-infected HaCaT cells and uninfected HaCaT cells. The expression of YY1 was detected by using western blot analysis. Cells in Lentivirus-YY1-HaCaT group and HaCaT-YY1 group were further divided into two subgroups according to the calcium concentration in culture medium, cells were either cultured in low-calcium medium (0.12 mmol/L) for 48 hours, or cultured in low-calcium medium (0.12 mmol/L) for 24 hours and in high-calcium medium (0.35 mmol/L) for additional 24 hours. Keratin K1, K10, K14, and involucrin, filaggrin and loricrin after over-expression of YY1 were detected with western blot analysis. RESULTS AND CONCLUSION: The HaCaT cells were successfully infected with lentivirus-YY1, and we obtained over-expression of YY1 protein in monoclonal cell lines under high-calcium concentrations, the over-expressed YY1 could decrease the expression of K1, involucrin and loricrin, thereby preventing the process of epidermal keratinocytes and maintaining the cells in an undifferentiated state. Lentivirus can efficiently infect human immortalized epidermal cell HaCaT, and YY1 may the important factor of inhibiting the differentiation of basal epidermal cells and maintaining the undifferentiated proliferation status. 中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 全文链接： Figures and Tables | References | Related Articles | Metrics

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Hypoxia effects on the chondrogenic differentiation of three-dimensional co-cultured adipose-derived stem cells and articular chondrocytes Dai Bing, Xu Hai-ting, Jin Hai-dong, Chen Hui, Cai Jian-wu, Fan Shi-yang, Pan Jun 2014, 18 (29):  4630-4635.  doi: 10.3969/j.issn.2095-4344.2014.29.007 Abstract ( 313 )   PDF (2164KB) ( 351 )   Save  BACKGROUND: Many in vivo and in vitro experiments indicate that hypoxic co-cultures promote stem cells differentiate into chondrocytes. OBJECTIVE: To evaluate the influence of hypoxia on the chondrogenic differentiation of three-dimensional co-cultured adipose-derived stem cells and articular chondrocytes. METHODS: Adipose-derived stem cells and articular chondrocytes were mixed at the ratio of 3:1, then the mixed cells were seeded onto poly(lactic-co-glycolic acid)-gelatin scaffold at the ultimate concentration of 5.0×1010/L. The cells were cultured in normoxia (20% O2) and hypoxic (5% O2) conditions for 6 weeks. After culture, hematoxylin and eosin staining was performed for histological structure analysis, and alcian blue staining was used to evaluate glycosaminoglycan synthesis. Type II collagen expression was detected by immunohistochemistry staining. The content of DNA, glycosaminoglycan and hydroxyproline in the scaffold-cell complex was measured. RESULTS AND CONCLUSION: In the hypoxia group, hematoxylin-eosin staining showed the formation of massive cells and extracellular matrix; alcian blue staining showed massive glycosaminoglycan formation; immunohistochemistry staining detected strongly positive expression of collagen type II, the content of DNA, glycosaminoglycan and hydroxyproline was higher than the normoxia group. Hypoxia promotes in vitro chondrogenic differentiation of co-cultured adipose-derived stem cells and articular chondrocytes. 中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 全文链接： Figures and Tables | References | Related Articles | Metrics

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Construction of pcDNA3-Endo eukaryon expression plasmid and angiogenesis inhibition in vitro Shao Jia-jia, Yu Yin, Jiang Tao 2014, 18 (29):  4636-4641.  doi: 10.3969/j.issn.2095-4344.2014.29.008 Abstract ( 452 )   PDF (728KB) ( 821 )   Save BACKGROUND: The eradication therapy of glioma is the major problem, and anti-angiogenesis therapy is a potential treatment of glioma. OBJECTIVE: To confirm the inhibiting effect of endostatin on angiogenesis in vitro, and to lay the foundation in inhibiting the growth of tumor by endostatin in the future. METHODS: Endostatin mRNA was extracted from the liver of Wistar rats by Trizol and endostatin cDNA was synthesized by RT-PCR. Endostatin cDNA and pcDNA3 were connected and pcDNA3-Endo recombined plasmid was constructed successfully. The recombinant pcDNA3-Endo was transfected into bone marrow mesenchymal stem cells by Lipofectamine. The expression of endostatin was identified by RT-PCR and western blot analysis. Endostatin proteinum activity was detected by ECV-304 cell proliferation inhibition experiment using MTT assay. The in vitro experiments were divided into four groups: recombinant plasmid group, vector plasmid group, liposome control group and blank control group. RESULTS AND CONCLUSION: PcDNA3-Endo eukaryon expression plasmid was constructed successfully. Endostatin gene can be transcribed and expressed effectively in vitro by pcDNA3-Endo plasmid. The growth of ECV-304 cell was inhibited obviously by pcDNA3-Endo. The growth of vascular endothelial cells can be inhibited obviously by endostatin gene. 中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 全文链接： Figures and Tables | References | Related Articles | Metrics

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Changes of blood rheology indexes and bifurcation shearing force after exercise Tian Gui-ling 2014, 18 (29):  4642-4646.  doi: 10.3969/j.issn.2095-4344.2014.29.009 Abstract ( 306 )   PDF (639KB) ( 335 )   Save BACKGROUND: The abnormal blood rheology after exercise is one of the leading causes of exercise fatigue, and also a risk factor of exercise-induced cardiovascular disease. OBJECTIVE: To analyze the effect of exercise on blood rheology indexes and vascular dynamics. METHODS: The blood vessel models were established. The bifurcation shearing force was analyzed in the exercise modes of one-time exhaustive exercise and systemic exercise. The blood rheology indexes among 30 healthy volunteers were detected. Based on the consulting literatures, we observed the effect of exercise mode on blood rheology through experiment and theoretical analysis, and investigated the correlation between exercise mode and bifurcation shearing force. RESULTS AND CONCLUSION: One-time exhaustive exercise had adverse effects on blood rheology, while system exercise significantly changed blood rheology. The bifurcation vascular shear stress was less than 0.6 Pa after one-time exhaustive exercise, and about 0.6-1.0 Pa after system exercise. Therefore, one-time exhaustive exercise induces vascular disease; system exercise can prevent vascular diseases. 中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 全文链接： Figures and Tables | References | Related Articles | Metrics

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Distribution of joint isokinetic muscle strength in shot putters Lou Yan-tao 2014, 18 (29):  4647-4652.  doi: 10.3969/j.issn.2095-4344.2014.29.010 Abstract ( 409 )   PDF (630KB) ( 682 )   Save BACKGROUND: At present, the detections of muscular strength of the main joints in shot putters by using isokinetic system are rarely reported. OBJECTIVE: To find out the characteristics of muscular strength of the main joints in shot putters under different speed, and explore the existing shortcomings. METHODS: Using German ISOMED2000 isokinetic test system, we detected the isokinetic muscular strength of knee joint, hip joint, waist-back segment, and shoulder joint of the men’s shot put champion in the 11th National Games, at slow 60°/s and fast 180°/s. RESULTS AND CONCLUSION: (1) H/Q ratios of the left and right side of knee joint were 25.6%, 39.4% in slow speed, while 28.5%, 29.8% in high speed. In addition, flexor muscle group had small muscular strength. (2) The muscular strength of the right extensor group of hip joint was as much 2.11 and 1.87 times respectively as left side in high and slow speed. Meanwhile, flexor muscle group was 1.25 and 1.64 times and significance of right hip was greater than the left. (3) H/Q ratios of the waist and back joints were 93.3% and 84.3% respectively in high and low speed. (4) Under natural flexion of shoulder joint, myodynamia flexion in slow speed was as much 1.27 and 1.34 times respectively as the left, while 1.40 and 1.92 times respectively as the left in fast speed. (5) According to relative weight of the human body model, when each segment was compared to knee as a benchmark, the knees, hips, body and shoulder joints were 1:2.38:3.30:0.70. The findings indicate that, (1) in the knees joint, not only absolute myodynamia of flexor and extensor groups, but also myodynamia of fast flexor groups are weak, the equipment of myodynamia group is not reasonable as well. (2) The body myodynamia reaches the peak in the right side of extensor group, while rapid extensor myodynamia at the left and right side is very weak, therefore, we should increase the training of waist and back extensor, especially muscle strength training and extension training at the slow and high speed. (3) As extensor group on the right side is weak, we should strengthen the absolute extensor group muscle power of right shoulder and absolute muscle strength of flexion muscle group. 中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 全文链接： Figures and Tables | References | Related Articles | Metrics

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Apoptosis of human embryonic cerebral cortex neurons transfected with human telomerase reverse transcriptase Wu Ling-zhi, Li Shui-bin, Cheng Gang-wei, Wang Hua-qiao, Kong Ling-ping 2014, 18 (29):  4653-4657.  doi: 10.3969/j.issn.2095-4344.2014.29.011 Abstract ( 241 )   PDF (1833KB) ( 355 )   Save BACKGROUND: Human telomerase reverse transcriptase recombinant adenovirus transfection could promote the survival and inhibit the apoptosis of primary cultured human embryonic cerebral cortex neurons. OBJECTIVE: To observe the effects of human telomerase reverse transcriptase on the apoptosis of human embryonic cerebral cortex neurons induced by amyloid β-protein invention. METHODS: Primary cultured cerebral cortex neurons of human embryo were divided into three groups, namely control group, amyloid β-protein (25-35) group, and human telomerase reverse transcriptase group. Except the control group, the cells were cultured for 144 hours and intervened with 5 μmol/L amyloid β-protein (25-35) for 24 hours. Furthermore, the cells in human telomerase reverse transcriptase group were transfected with human telomerase reverse transcriptase group at 72 hours after intervention of 5 μmol/L amyloid β-protein (25-35). RESULTS AND CONCLUSION: After human embryonic cerebral cortex neurons were cultured for 7 days, the apoptotic cells were found in three groups. The apoptosis of neurons was obviously increased after intervention of amyloid β-protein (25-35). Human telomerase reverse transcriptase transfection could prevent the apoptosis of human embryonic cerebral cortex neurons induced by amyloid β-protein (25-35). 中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 全文链接： Figures and Tables | References | Related Articles | Metrics

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Tissue engineered artificial nerves repair sciatic nerve defect in rats: an evaluation using horseradish peroxidase retrograde tracer technique Zhang Cai-shun, Lv Gang 2014, 18 (29):  4658-4662.  doi: 10.3969/j.issn.2095-4344.2014.29.012 Abstract ( 369 )   PDF (2154KB) ( 400 )   Save BACKGROUND: We have previously prepared acellular nerve graft and implanted bone marrow mesenchymal stem cells into the graft, to successfully construct tissue engineered artificial nerves. OBJECTIVE: Horseradish peroxidase nerve retrograde tracer technique was used to evaluate protective effects on sensory neurons following sciatic nerve defect bridging with tissue engineered artificial nerves constructed by acellular nerve graft and bone marrow mesenchymal stem cells in rats. METHODS: Adult, clean, healthy, male Sprague-Dawley rats were randomly assigned to three group: (1) Experimental group: Rat sciatic nerve detect was bridged by acellular nerve graft combined with bone marrow mesenchymal stem cells; (2) Blank control group: Rat sciatic nerve defect was bridged by acellular nerve graft; (3) Autologous nerve control group: Rat sciatic nerve defect was bridged by autologous nerve transplantation. Regeneration of sensory neurons in the spinal dorsal root ganglia was assessed using horseradish peroxidase nerve retrograde tracer technique at 12 weeks following surgery. RESULTS AND CONCLUSION: Sensory neuron regeneration in the spinal dorsal root ganglia at 12 weeks following surgery was better in the experimental group compared with blank control group. No significant difference was detected between experimental group and autologous nerve control group. S-100 immunohistochemical staining in plantar skin showed brown positive reaction in each group. These findings indicate that tissue engineered artificial nerves constructed by acellular nerve graft and bone marrow mesenchymal stem cells have protective effects on sensory neurons in the spinal dorsal root ganglia, and can promote the recovery of sensory function and repair sciatic nerve defect in rats. 中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 全文链接： Figures and Tables | References | Related Articles | Metrics

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Long-term culture of hepatic oval cells in rats in vitro Yin Shou-xin, Zhou Zhen, Ma Mei-xue, Yang Jing-bo, Yang Dong-ye 2014, 18 (29):  4663-4668.  doi: 10.3969/j.issn.2095-4344.2014.29.013 Abstract ( 350 )   PDF (1956KB) ( 273 )   Save BACKGROUND: Hepatic oval cells are recognized as stem/progenitor cells currently, however, long-term culture of hepatic oval cells can inevitably result in the loss of cell activity. OBJECTIVE: To explore a long-term culture method of hepatic oval cells in vitro. METHODS: Partially hepatectomized rat model was established by using 2AAF/PH. The regenerated liver was digested by collagenase, and hepatic oval cells were isolated and purified by density gradient centrifugation and identified by immunocytochemistry. Hepatic oval cells were cultured in a medium containing epidermal growth factor and leukaemia inhibitory factor. Then epidermal growth factor and leukaemia inhibitory factor were removed after several months, and its ability of maintaining stem/progenitor cell activities was determined based on morphology and molecular markers. RESULTS AND CONCLUSION: Both hepatocyte marker ALB and biliary epithelial cell marker CK-19 were found after hepatic oval cells were cultured in a medium containing epidermal growth factor and leukaemia inhibitory factor for 4 months. While in the absence of epidermal growth factor and leukaemia inhibitory factor, the expression of fetal liver marker AFP was decreased quickly. These results indicated that hepatic oval cells could expand in a medium containing epidermal growth factor and leukaemia inhibitory factor and maintain stem/progenitor cell activities for a long time. 中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 全文链接： Figures and Tables | References | Related Articles | Metrics

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Optimization of N2a cell transfection mediated by liposome Zhao Yun-he, Wang Ruo-nan, Yang Gui-jiao, Lu Li 2014, 18 (29):  4669-4674.  doi: 10.3969/j.issn.2095-4344.2014.29.014 Abstract ( 604 )   PDF (753KB) ( 1314 )   Save BACKGROUND: Cationic liposome-mediated cell transfection is reliable and repeatable. However the transfection efficiency is often low. OBJECTIVE: To study the optimized methods for gene transfection mediated by liposome into N2a cells (mouse neuroblastma cells). METHODS: Using traditional adherent method and improved suspension method, 500 ng recombinant plasmid pcDNA3-GFP carrying green fluorescence protein was transfected into N2a cells in 24-well culture plate, which was mediated by 1.5 μL Lipofectamine™ LTX Reagent. The expression of green fluorescent protein was observed by inverted fluorescence microscope, and the transfection efficiencies at different transfection ways were calculated. By using improved suspension transfection method, 500 ng plasmid DNA was transfected with different doses of Lipofectamine™ LTX Reagent (1.0, 1.5, 2.0, 2.5 μL). The optimal ratio of liposome and DNA was explored. RESULTS AND CONCLUSION: The transfection efficiency of suspension transfection method was significantly higher than that of the tranditional adherent method (P < 0.01) when using 1.5 μL liposome/500 ng DNA. The transfection efficiency of the 1.0, 1.5, 2.0, 2.5 μL Lipofectamine™ LTX on 500 ng plasmid DNA was respectively (76.60±3.85)%, (80.00±4.17)%, (88.00±5.89)%, (54.96±4.23)%. It showed the 500 ng DNA and 2.0 μL liposome achieve the highest transfection efficiency. 中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 全文链接： Figures and Tables | References | Related Articles | Metrics

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Construction and identification of pIRES2-GDNF-VEGF165 bicistronic eukaryotic expression vector Li Bing-nan, Li Wei-dong, Lin Jun-tang, Feng Hui-gen 2014, 18 (29):  4675-4682.  doi: 10.3969/j.issn.2095-4344.2014.29.015 Abstract ( 313 )   PDF (425KB) ( 559 )   Save BACKGROUND: Human glial cell line-derived neurotrophic factor (GDNF) and vascular endothelial growth factor 165 (VEGF165) are essential genes for cell differentiation. OBJECTIVE: To construct and identify pIRES2-GDNF-VEGF165 bicistronic eukaryotic expression vector. METHODS: Human GDNF genes were obtained from the genomic DNA of human peripheral blood mononuclear cells by PCR. Then the GDNF cDNA fragment was inserted into the multiple cloning sites of pIRES2-EGFP, to generate the bicistronic eukaryotic expression plasmid pIRES2-GDNF-EGFP. The VEGF165 gene was obtained from pIRES2-VEGF165-EGFP plasmid by twin PCR. Then VEGF165 cDNA fragment was cloned into the pIRES2-GDNF-EGFP, instead of EGFP, to create a double gene co-expressing vector plasmid pIRES2-GDNF-VEGF165 containing internal ribosome entry sites. Then pIRES2-GDNF-VEGF165 was used to transfect HEK293 cells. RT-PCR and western blot analysis were performed to test the co-expression of double genes. RESULTS AND CONCLUSION: DNA sequencing analysis demonstrated that the GDNF and VEGF165 were exactly consistent with the sequence recorded in the GenBank. The size of GDNF gene was 636 bp and the size of VEGF165 gene was 576 bp. Enzyme digestion analysis indicated that, pIRES2-GDNF-VEGF165 bicistronic eukaryotic expression vector inserted GDNF band by Bgl II/Bam HI, inserted IRES-VEGF165 fragment by Bam HI/Not I, and inserted GDNF-IRES-VEGF165 fragment by Bgl II/Not I. RT-PCR and western blot analysis showed that, after HEK293 cells were transfected with pIRES2-GDNF-VEGF165, double genes were expressed at the mRNA and protein levels. The pIRES2-GDNF-VEGF165 bicistronic eukaryotic expression vector is successfully constructed. 中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 全文链接： Figures and Tables | References | Related Articles | Metrics

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Effect of intrathecal injection of dexmedetomidine on protein kinase C expression of spinal dorsal horn neurons in a rat model of chronic neuralgia Deng Hai-hong, Ma Song-mei, Xiao Xiao-shan 2014, 18 (29):  4683-4688.  doi: 10.3969/j.issn.2095-4344.2014.29.016 Abstract ( 416 )   PDF (2668KB) ( 277 )   Save BACKGROUND: Dexmedetomidine is an efficient, highly selective alpha-2 adrenergic receptor agonist, with sedative, analgesia and anti-anxiety effects, it has little impact on the respiration. OBJECTIVE: To observe the analgesic effect induced by intrathecal injection of dexmedetomidine in rat model of spared nerve injury. METHODS: A total of 60 male Sprague-Dawley rats were randomly divided into three groups (n=12): normal control group, dexmedetomidine group and saline group. Except for the normal control group, spared nerve injury model was established in the rats of dexmedetomidine group and saline group. Dexmedetomidine group was treated with intrathecal injection of dexmedetomidine 3 μg/kg every day within 14 days after injury. Saline group was given equal volume of saline for 14 days. The thermal withdrawal latency and mechanical withdrawal threshold were measured respectively before injury, after injury, before injection, and 2, 7, 14 days after intrathecal injection. Four rats were sacrificed in each group at day 2, 7 and 14 after injection, and the lumbar segments (L4-6) of the spinal cord were removed. Real-time RT-PCR and western blot analysis were used to determine the expression of protein kinase C mRNA and protein in the spinal dorsal horn neurons. Hematoxylin-eosin staining was performed to detect the morphology of the spinal dorsal horn neurons and immunohistochemistry staining was carried out to assess the expression level and distribution of protein kinase C. RESULTS AND CONCLUSION: The thermal withdrawal latency and mechanical withdrawal threshold in dexmedetomidine group and saline group were significantly decreased compared with normal control group before or after injection (P < 0.05). However, both the thermal withdrawal latency and mechanical withdrawal threshold in dexmedetomidine group after intrathecal injection were significantly higher than those in saline group (P < 0.05). The protein kinase C expression in spinal dorsal horn neurons was significantly decreased in dexmedetomidine group compared with saline, and reached to the most lowest levels as normal control group on 14 days after injection. Moreover, the apoptosis of spinal dorsal horn neurons in dexmedetomidine group was lighter than that in saline group, and was similar to the morphology of neurons in normal control group on 14 days after injection. Intrathecal injection of dexmedetomidine could attenuate the hyperalgesia induced by spared nerve injury, which might be associated with the inhibition of protein kinase C expression in spinal dorsal horn. 中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 全文链接： Figures and Tables | References | Related Articles | Metrics

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Differential expression of angiogenic growth factors in cerebrovascular malformations Zhu Guo-hua, Yang Zhen-cun, Dang Mu-ren, Maimaitili, Wang Xin, Serickduysenbi 2014, 18 (29):  4689-4694.  doi: 10.3969/j.issn.2095-4344.2014.29.017 Abstract ( 279 )   PDF (2479KB) ( 364 )   Save BACKGROUND: Cerebral vascular malformations are the leading cause of hemorrhagic apoplexy in young adults, and the rupture and bleeding of malformed vessels may cause severe neurological dysfunction. The mechanism of cerebral vascular malformations remains unclear. Modern molecular biology studies have shown that, angiogenic growth factors are abnormally expressed in cerebral vascular malformations. OBJECTIVE: To evaluate differences in the expression of angiogenic growth factors in cerebral vascular malformations, and discuss the possible relationship between cerebral vascular malformations and angiogenic growth factors. METHODS: Fifty patients with cerebral vascular malformations and fifty patients with intracerebral hemorrhage were included in this study. The expressions of angiogenic growth factor (vascular endothelial growth factor and transforming growth factor-α) in the cerebral vascular malformation specimens and the normal superficial temporal artery specimens were detected with immunohistochemical staining. RESULTS AND CONCLUSION: In the normal superficial temporal artery of intracerebral hemorrhage patients, no expression of vascular endothelial growth factor and transforming growth factor-α was found; in the vascular malformations, they were highly expressed (P < 0.05). Compared with normal blood vessels, vascular endothelial growth factor and transforming growth factor-α expression was significantly increased in patients with cerebral vascular malformations. 中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 全文链接： Figures and Tables | References | Related Articles | Metrics

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Specific gene expression of osteoclasts under different oxygen tension Liang Jing, Zhou Qi, Wei Li, Hu Fang-qiong, Wang Jun 2014, 18 (29):  4695-4700.  doi: 10.3969/j.issn.2095-4344.2014.29.018 Abstract ( 594 )   PDF (1934KB) ( 350 )   Save BACKGROUND: Preliminary studies of our research group have confirmed that the proliferation of preosteoclasts and the differentiation and function of osteoclasts could be inhibited when they were cultured in lower oxygen tension even hypoxia (2% O2), but the gene expression of osteoclasts cultured in vitro have not been reported. OBJECTIVE: To examine the effect of oxygen tension on specific gene expression of osteoclasts in vitro and explore the mechanism of osteoclast differentiation influenced by oxygen tension. METHODS: The preosteoclasts were induced with 10 μg/L macrophage colony stimulating facto and 10 μg/L soluble receptor activator of nuclear factor-κB ligand into mature osteoclasts. Then the osteoclasts were cultured in normoxia, tissue oxygen and hypoxia (20%, 7%, 2% O2) respectively. Cells were then stained for tartarate-resistant acid phosphatase to assess osteoclastic formation. Cells were collected at 1, 2, 3, 4, 5, 6, 7 days after culture respectively. The soluble receptor activator of nuclear factor-κB ligand, tumor necrosis factor receptor-associated factor 6, tartarate-resistant acid phosphatase, and cathepsin K mRNA expression levels were determined using real-time quantitative PCR. RESULTS AND CONCLUSION: The number of osteoclasts positive for tartarate-resistant acid phosphatase in the hypoxia was significantly lower than that in the tissue oxygen and normoxia (P < 0.05). Under different oxygen tension, the mRNA expression levels of soluble receptor activator of nuclear factor-κB ligand in osteoclasts maintained unchanged. The mRNA expression levels of tumor necrosis factor receptor-associated factor 6 reached the peak at 5 days after culture in tissue oxygen and normoxia (P < 0.05). The mRNA expression time of tartarate-resistant acid phosphatase and Cathepsin K were delayed accompanied by decreased oxygen tension, but the maximum were maintained in tissue oxygen. Compared with normoxia and hypoxia, osteoclasts cultured in tissue oxygen are more prone to differentiate and maintain the activity and functions. 中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 全文链接： Figures and Tables | References | Related Articles | Metrics

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Effects of telmisartan on endothelial progenitor cells and high sensitive C-reactive protein in patients with unstable angina pectoris Ni Wei 2014, 18 (29):  4701-4705.  doi: 10.3969/j.issn.2095-4344.2014.29.019 Abstract ( 313 )   PDF (754KB) ( 333 )   Save BACKGROUND: Atherosclerotic plaque is chronic low-level inflammation in human body. Telmisartan can lower blood pressure, protect the target organs and inhibit inflammation, also partially activate peroxisome proliferator-activated receptor γ, increase the number of progenitor cells, and inhibit inflammation of vascular wall cells. OBJECTIVE: To observe the effects of telmisartan on endothelial progenitor cells and high-sensitivity C-reactive protein in peripheral blood of patients with unstable angina pectoris. METHODS: 200 patients with unstable angina pectoris who consulted the doctors of the First People’s Hospital of Neijiang from January 2012 to December 2013 were randomly divided into contrast group and telmisartan group, with 100 patients in each group. The general clinical data of each patient was collected. Peripheral venous blood was taken before treatment and 4, 8 weeks after treatment. Endothelial progenitor cells and high-sensitivity C-reactive protein level were detected. RESULTS AND CONCLUSION: At 4 and 8 weeks after treatment, the percentage of endothelial progenitor cells in telmisartan group was significantly higher than those before treatment and that of contrast group (P < 0.05). At 4 and 8 weeks after treatment, the high-sensitivity C-reactive protein level was decreased significantly in telmisartan group compared with before treatment. The telmisartan group had a significantly lower level than the contrast group (P < 0.05). Telmisartan can promote the proliferation of endothelial progenitor cells and restoration of damaged vessels. It can also reduce high-sensitivity C-reactive protein level and inhibit the inflammatory reaction. 中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 全文链接： Figures and Tables | References | Related Articles | Metrics

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Combined treatment of HUOXUEBUSHEN Decoction and bone impaction grafting for nontraumatic osteonecrosis of the femoral head Wei Biao-fang, Sun Bing-yin 2014, 18 (29):  4706-4711.  doi: 10.3969/j.issn.2095-4344.2014.29.020 Abstract ( 335 )   PDF (744KB) ( 341 )   Save BACKGROUND: The treatment of osteoporosis of the femoral head relies on the reconstruction and improvement of blood supply in the femoral head, maintenance of femoral head structure, restoration of mechanical environment and blood supply in femoral head. Traditional Chinese medicine treatment is very advantageous. Bone impaction grafting through a femoral neck window at high location can restore the mechanical environment in femoral head. OBJECTIVE: To investigate clinical efficacy of combined treatment of HUOXUEBUSHEN decoction and bone impaction grafting through a femoral neck window at high location for non-traumatic osteoporosis of the femoral head. METHODS: This study is a prospective controlled trial. The patients with non-traumatic osteoporosis of the femoral head were divided into two groups: treatment group (n=52, 60 hips) and control group (n=51, 60 hips). The treatment group was given a combined treatment of HUOXUEBUSHEN decoction and bone impaction grafting through a femoral neck window (the decoction was prepared in People’s Hospital of Linyi, China). The control group underwent simple bone impaction grafting through a femoral neck window. All the involved patients were followed up for 12 months, Harris hip score and the excellent-good rate at the last follow-up were used to evaluate clinical efficacy. RESULTS AND CONCLUSION: The Harris score, pain degree, and joint range of motion scores in the treatment group were higher than that in control group (P < 0.01), but joint deformities and functional scores showed no significant differences between two groups (P > 0.05). The excellent-good rate in the treatment group was significantly higher than that in control group (88%, 75%;χ2=4.91, P < 0.05). The combined treatment of HUOXUEBUSHEN decoction and bone impaction grafting through a femoral neck window at high location is effective in the treatment of non-traumatic osteonecrosis of the femoral head, and is better than simple bone impaction grafting at alleviating pain and improving joint range of motion. 中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 全文链接： Figures and Tables | References | Related Articles | Metrics

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Detection of early root fracture in maxillary molar: cone beam CT versus periapical radiographs Luo Rong, Chen Guang-sheng 2014, 18 (29):  4712-4716.  doi: 10.3969/j.issn.2095-4344.2014.29.021 Abstract ( 281 )   PDF (653KB) ( 361 )   Save BACKGROUND: Detection of root fracture is difficult since the fracture line is not apparent. A recent systematic review concluded that there was a lack of evidence-based data regarding the diagnostic accuracy of conventional radiographic signs for root fracture. OBJECTIVE: To compare the diagnosis of maxillary molars with early root fracture by periapical film and cone-beam CT retrospectively. METHODS: Forty teeth diagnosed as early root fracture of maxillary molars from 38 patients were included. Results of periapical film and cone-beam CT were compared. RESULTS AND CONCLUSION: Among the 40 suspected teeth, 19 teeth were diagnosed as early root fracture by periapical film. The fracture line was located in the mesiobuccal root of maxillary molars. However, 30 teeth were diagnosed as early root fracture by cone-beam CT. Among them, 23 teeth had fracture lines in mesiobuccal root and 7 teeth had fracture line in palatal root. One tooth could not be found any fracture sign in cone-beam CT. After 6 months of conservative treatment, this tooth was extracted for palatal root fracture. Compared with periapical film, cone-beam CT is better in early root fracture diagnosis, especially for the teeth with fracture line in palatal root. 中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 全文链接： Figures and Tables | References | Related Articles | Metrics

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Repairing critical-size segmental defects: morphology of tissue engineering bone scaffolds and its effects on cell loading      Zeng Xian-li, Yang Chun-lu 2014, 18 (29):  4717-4723.  doi: 10.3969/j.issn.2095-4344.2014.29.022 Abstract ( 363 )   PDF (719KB) ( 534 )   Save BACKGROUND: Tissue engineering bone application for repairing critical-size segmental defects is still in research stage. The ideal construction methods have not yet been found. OBJECTIVE: To review the research literatures on tissue engineering bone scaffold material, its shape and effect on the loading of seeding cells, seek appropriate engineered bone scaffolds which are capable of loading a large number of cells effectively and probably, and provide a new way of repairing segmental bone defects. METHODS: The first author performed a data retrieval of PubMed and Wanfang databases from 1994 to 2013, to search the articles addressing the construction method of tissue engineering bone scaffold, and reviewed the literatures systematically. RESULTS AND CONCLUSION: A total of 379 references were retrieved, including 161 articles in Chinese and 218 articles in English. According to the inclusion and exclusion criteria, 53 articles were finally involved in the analysis. The analysis results indicated that, the needed volume of bone tissue engineering scaffolds for critical-sized section bone defect reconstruction is big, which needs to load a huge number of seed cells. If there is no suitable forms and shapes for cell adhesion, the property of so-called engineered bone is similar to pure artificial bone implants. The effective load of seed cells on engineering bone scaffold material and keeping the activity is the first step in clinical practice, as well as the important guarantee for loading bioactive seed cells. Hence, a more simple and accurate detection method for loading cell quantity is needed. Looking into the retrieved content, effective load cell quantity and its bioactivity are detected by indirect methods, supporting the effectiveness of cell seeding. Some methods can guarantee the cell quantity and seeding pattern, the real load is unknown as well as the activity. Fabricating engineering bone scaffold into special form and shape are easy to effective seeding, proliferation and maintaining the biomechanical performance, inducing osteogenesis, and finally detecting the load cell quantity and activity on the scaffold through the simple and direct method. 中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 全文链接： Figures and Tables | References | Related Articles | Metrics

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Development of articular cartilage repair technique Ruan Zheng, Yin Qing-shui, Zhang Yu 2014, 18 (29):  4724-4729.  doi: 10.3969/j.issn.2095-4344.2014.29.023 Abstract ( 587 )   PDF (591KB) ( 433 )   Save BACKGROUND: Autologous cartilage has a poor self-repair effect due to low chondrocyte density, low metabolism rate and no blood supply. OBJECTIVE: To summarize the recent study about tissue engineering techniques and surgical treatment for cartilage injury. METHODS: A computer-based online retrieval of PubMed database was performed by the first author for articles published between January 1992 and December 2013. The key words are “articular cartilage, injury, tissue engineering, repair” in English. According to the inclusion and exclusion criteria, 61 literatures were included into the final analysis. RESULTS and CONCLUSION: The current clinical treatment of articular cartilage injury includes joint debridement, mosaicplasty, perichondrium transplantation and autologous chondrocyte implantation. However, their long-term result is unsatisfactory. One reason for limited clinical success is that new cartilage can be formed at the site of a defect, and the repaired tissue canot compare with the autologous cartilage in mechanical property. Tissue engineering technique is still a hot topic in recent years, because it can potentially induce autologous cartilage formation. Through endogenous or ectogeneous seed cells and inducting factor and nutrient factors, tissue engineering technique can be applied to induce the self-repair of articular cartilage, thus regenerating into hyalinc cartilage with the similar even same biological property. How to simplify the treatment protocols and reduce treatment cost is the key to promote cartilage repair. 中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 全文链接： Figures and Tables | References | Related Articles | Metrics

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Glucocorticoids-induced osteonecrosis of the femoral head: adipogenic differentiation and treatment progress Liu Bin, Li Gang, Xu Bo, Liu Guo-yan 2014, 18 (29):  4730-4735.  doi: 10.3969/j.issn.2095-4344.2014.29.024 Abstract ( 325 )   PDF (606KB) ( 321 )   Save BACKGROUND: Glucocorticoids may induce local bone trabecular and bone marrow necrosis, femoral head collapse and deformation, thus resulting in hip dysfunction. However the pathological mechanisms and treatment of glucocorticoids-induced osteonecrosis of the femoral head remain unclear, and the pathogenesis mechanisms are controversial. The current studies focus on the understanding of the pathological mechanisms. OBJECTIVE: To summary the research progress of adipogenic differentiation theory of glucocorticoids-induced osteonecrosis of the femoral head and the treatment. METHODS: The first author searched literature from CNKI and PubMed database from 1988 to 2010, by using the key words is “Glucocorticoids, osteonecrosis of femoral head, bone marrow stromal stem cells, adipogenic differentiation, differentiation factors, treatment, choices” in English, and “glucocorticoids induced osteonecrosis of femoral head, adipogenic differentiation, treatment, research progress” in Chinese. Articles regarding the adipogenic differentiation theory of glucocorticoids-induced osteonecrosis of the femoral head and the treatment were included. RESULTS AND CONCLUSION: A total of 112 literatures were screened out, according to inclusion and exclusion criteria for literature screening, 54 articles were included. Modern researches emphasize the cell and molecular biology level, and show that the biological base of glucocorticoids-induced osteonecrosis of the femoral head is abnormal adipogenic differentiation of bone cells, glucocorticoids cause the variations of adipogenic differentiation factors, leading to adipogenic differentiation of bone marrow stromal cells. But glucocorticoids affects multiple differentiation factors, it may cause great error in the evaluation of the pathogenesis of glucocorticoids-induced osteonecrosis of the femoral head purely from one factor. The abuse of glucocorticoids is the leading cause for the osteonecrosis of femoral head. Further studies are needed to investigate the pathogenesis of osteonecrosis of the femoral head and treatment programs. 中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 全文链接： Figures and Tables | References | Related Articles | Metrics

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Erosion prevention and remineralization of human teeth Wu Rui, Shi Mao-yu, Zheng Jing 2014, 18 (29):  4736-4741.  doi: 10.3969/j.issn.2095-4344.2014.29.025 Abstract ( 686 )   PDF (743KB) ( 409 )   Save BACKGROUND: With the consumption of acidic beverages, dental erosion becomes more serious. Erosion is related to direct loss of dental hard tissue, and causes dental over-abrasion, thus great threatening the dental health. OBJECTIVE: To review the research work on the erosion of human teeth, erosion prevention, and teeth remineralization. METHODS: A computer-based online retrieval of CNKI, Elsevier and Wiley databases between 1990 and 2013 was performed for articles abut human teeth erosion, erosion prevention and teeth remineralization. After repeated or old literatures were excluded, 58 literatures were included in the analysis. RESULTS AND CONCLUSION: Current studies on dental erosion included three aspects, firstly, clinical observation of the symptoms and cause of dental erosion; secondly, the erosiveness of common acidic agents; and thirdly, the prevention of dental erosion. Many researchers focused on the prevention of dental erosion. It was suggested that dental erosion could be prevented through adjusting and controlling oral environment, enhancing the erosiveness of acidic beverages, and increasing the anti-erosion capacity of teeth. It should be noted that tooth erosion and friction/wear often occur simultaneously in the mouth. Therefore, future research should pay attention to the interaction mechanism of erosion, friction and wear of human teeth. 中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 全文链接： Figures and Tables | References | Related Articles | Metrics

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Single-row versus double-row suture in rotator cuff repair: a meta-analysis Zhuang Ze, Lu Hua-ding, Chen Yu-xian, Peng You, Ren Jian-hua, Wang Kun 2014, 18 (29):  4742-4751.  doi: 10.3969/j.issn.2095-4344.2014.29.026 Abstract ( 510 )   PDF (739KB) ( 347 )   Save BACKGROUND: Single-row and double-row suture method are commonly used in the rotator cuff repair. Previous studies have shown that, double-row suture is not better than single-row suture in clinics. OBJECTIVE: To compare clinical outcomes of single-row suture and double-row suture for rotator cuff repair, and evaluate the difference of therapeutic efficacy between two methods. METHODS: A computer-based search was performed in the Medline (from January 2003 to February 2014), EMBASE (from January 2003 to February 2014) and Cochrane library (February 2014). According to the inclusion and exclusion criteria, all the randomized controlled studies addressing the outcome of single-row repair and double-row repair techniques were included in this meta-analysis. The methodological quality of each study was judged and a meta-analysis was conducted using Revman5.0. The preoperative and postoperative differences between the Constant score, American Shoulder and Elbow Surgeons (ASES) score, University of California, Los Angeles (UCLA) score, the re-rupture rate and the muscle strength were compared. The forest chart was used to compare the data between two groups, and the funnel plot was finished to detect the publication bias. RESULTS AND CONCLUSION: A total of 10 randomized controlled trials (Levels I, II) were included. Meta-analysis showed that, there was no statistically significant difference in the Constant, ASES and UCLA scores in the double-row group and the single-row group before and after treatment. In the postoperative follow-up, double-row group had a lower re-rupture rate and a higher abductor muscle strength than single-row group. When the rotator cuff tear was less than 3 cm, double-row group had no significant difference with the single-row suture group. While in the over 3-cm tear group, double-row group showed better results than the single-row suture group on the Constant scpre, ASES score and UCLA score. Double-row suture has a low re-rupture rate than single-row suture in rotator cuff injury, and could achieve better abduction muscle strength. There is no significant difference in the functional score between double-row suture and single-row suture in the rotator cuff tear of less than 3 cm, while in the over 3-cm tear, double-row suture could achieve better functional score. 中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 全文链接： Figures and Tables | References | Related Articles | Metrics

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Upper limb free mini-flap transplantation for repair of finger wounds   Cheng Jian-wen, Zhao Jin-min, Tan Zhen, Wang Jing-wei, Xue Ming-qiang 2014, 18 (29):  4752-4756.  doi: 10.3969/j.issn.2095-4344.2014.29.027 Abstract ( 411 )   PDF (690KB) ( 431 )   Save BACKGROUND: Recently, the clinical repair methods of irregular wound on fingers primarily include local pedicled flap and free mini-flap of upper extremities or lower extremities. OBJECTIVE: To discuss the application of free mini-flap derived from upper limb in repairing the wound on fingers. METHODS: From December 2010 to February 2014, 12 patients with irregular wounds on 12 fingers were selected from Department of Traumatic Orthopaedics and Hand Surgery, First Affiliated Hospital of Guangxi Medical University, China. The size of wounds ranged from 1.5 cm × 2.0 cm to 3.0 cm × 4.5 cm with different degrees of bone or tendon exposure. After the debridement, 12 patients were treated by free mini-flaps of upper limb. The donor sites were directly sutured. RESULTS AND CONCLUSION: Among the 12 patients, all the wounds at recipient and donor sites were healed at stage I, and the free mini-flags survival completely. All of patients were followed up for 3-6 months (average 4.5 months). Free mini-flags had good appearance and needn’t undergo secondary trimming. The active motion of fingers was improved dominantly. According to the criteria of Hand Surgery Association Society of Chinese Medical Association Society for the function evaluation of upper limb, three cases were excellent, eight were good, and only one was bad. The excellent and good rate was 91%. Free mini-flaps of upper limb are an ideal method for repair of wounds on fingers. It has no injury to normal tissue in hands, donor site is very secluded, and the short-term curative effect is good. 中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 全文链接： Figures and Tables | References | Related Articles | Metrics