Database construction and data analysis of von Willebrand factor and platelet glycoprotein Ib alpha mutations

doi:10.3969/j.issn.2095-4344.2014.29.002

Chinese Journal of Tissue Engineering Research ›› 2014, Vol. 18 ›› Issue (29): 4599-4604.doi: 10.3969/j.issn.2095-4344.2014.29.002

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Database construction and data analysis of von Willebrand factor and platelet glycoprotein Ib alpha mutations

Li Na¹, Li Qu-huan¹, Wang An-qi²

¹School of Bioscience and Engineering, South China University of Technology, Guangzhou 510006, Guangdong Province, China; ²School of Computer Science & Engineering, South China University of Technology, Guangzhou 510006, Guangdong Province, China

Revised:2014-05-04 Online:2014-07-09 Published:2014-07-09
Contact: Li Qu-huan, M.D., Associate professor, School of Bioscience and Engineering, South China University of Technology, Guangzhou 510006, Guangdong Province, China
About author:Li Na, Studying for master’s degree, School of Bioscience and Engineering, South China University of Technology, Guangzhou 510006, Guangdong Province, China
Supported by:
the National Natural Science Foundation of China for Youths, No. 31200705; Specialized Research Fund for the Doctoral Program of Higher Education, No. 20100172120042; Fundamental Research Funds for the Central Universities, No. 2014ZZ0051

Abstract

Abstract:

BACKGROUND: The interaction between von Willebrand Factor (VWF) and platelet surface receptor, platelet glycoprotein Ib alpha (GPIbα), plays a key role in the process of platelet adhesion, spread and aggregation. Currently, the database collecting VWF mutations is not complete and GPIbα database has not been constructed yet.

OBJECTIVE: The construction of VWF and GPIbα mutation database can help the researchers in this field to find the important mutation information of these two molecules quickly.

METHODS: For constructing VWF and GPIbα mutation database, data were collected from Uniprot, VWFdb and literatures by keywords search, the dynamics website was designed using MySQL+Apache+PHP combination.

RESULTS AND CONCLUSION: The database contains 341, 13 and 3 920 wild and mutation records of VWF, GPIbα, and A1 domain. The website connecting with backend database is comprised of the background introduction, the pathological pictures and data download page and so on. Especially, the information of mutations and virtual mutations can be searched and analyzed through search engine with sample and logical strategy. This database will be greatly beneficial to study VWF and GPIbα. Furthermore, it can offer some new therapeutic approaches to inhibit the binding of VWF and GPIbα, which serve as an attractive drug target in von Willebrand Disease.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

全文链接：

Key words: von Willebrand diseases, platelet glycoprotein GPIb-IX complex, mutant proteins, databases, protein

CLC Number:

R318

Li Na, Li Qu-huan, Wang An-qi. Database construction and data analysis of von Willebrand factor and platelet glycoprotein Ib alpha mutations[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(29): 4599-4604.

Figures/Tables 3

为了保证数据库内容的动态性、有效性，系统的登录内容包括主页面，背景介绍页面，病例图册页面，资料下载页面以及检索页面，见图2。主页面介绍VWF和GPIbα之间的相互作用，以及该相互作用如何介导在血液中自由流动的血小板在受损的血管壁上初始黏附、稳定黏附，最终形成血栓的过程(图3左)。此外，也提供了与相关生物数据库(NCBI、DDBJ、EMBL等)的各个链接，便于研究者的查询。背景介绍页面是将图片制成地图并设置成热点，当获取鼠标单击动作，即可打开详细介绍页面，当获取到鼠标双击的动作时，就关闭该图层，恢复原页面。该页面详细介绍了VWF和GPIbα分子对相关联的字段的定义，如血管性血友病的病因，临床特征，GPIbα多态性等。数据库信息检索是任何通用数据库管理系统的核心功能之一，也是衡量开发成功与否的重要条件之一。本数据库的检索页面提供条件组合检索和结合图表的动态条件检索两种方法，方便用户进行突变位点与疾病类型关联信息的查询及可视化网络显示查询。条件组合检索的实现是在html页面部分设置表单，点击search后传值到PHP页面。然后在PHP页面通过字符连接重新设置检索条件并最终输出结果。用户可以根据需要只选择一种条件，也可以选择2个条件或3个条件(图3右)。图像动态条件检索则是通过点击结构域相应区域，可直接获取检索结果，方便用户直观地看到检索结果。资料下载页面提供了数据库所有关于VWF和GPIbα突变条目信息的下载，病例图册页面则展示VWF和GPIbα分子对之间涉及的信号通路，结构解析等经典图像及其明确的出处，这些信息均有利于研究人员对VWF和GPIbα突变信息的相关研究。"

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Database construction and data analysis of von Willebrand factor and platelet glycoprotein Ib alpha mutations

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