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    16 July 2014, Volume 18 Issue 30 Previous Issue    Next Issue
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    Application of high viscosity bone cement in thoracolumbar osteoporotic compression fractures
    Feng Xin-min, Wang Jing-cheng, Zhang Liang, Tao Yu-ping, Yang Jian-dong, Cai Jun, Zhang Sheng-fei, Huang Ji-jun
    2014, 18 (30):  4757-4763.  doi: 10.3969/j.issn.2095-4344.2014.30.001
    Abstract ( 324 )   PDF (1026KB) ( 571 )   Save

    BACKGROUND: Vertebroplasty and kyphoplasty have been widely applied in the treatment of osteoporotic thoracolumbar compression fracture. However, cement leakage is a major problem in the application of this technology, especially for the vertebral posterior wall ruptured patients.
    OBJECTIVE: To investigate the therapeutic efficacy of high viscosity bone cement and vertebroplasty in the treatment of osteoporotic thoracolumbar compression fracture.
    METHODS: A retrospective study was conducted in 20 cases receiving high viscosity bone cement and vertebroplasty surgery for osteoporotic thoracolumbar compression fracture. Clinical outcomes were evaluated mainly with use of Visual Analog Scale for lower back pain. Function of lower back pain was assessed using Oswestry Disability Index questionnaire. Quality of life was evaluated using 36-Item Short Form Health Survey and Frankel score was applied to evaluate neurological function. The anterior vertebral height of the fractured vertebrae was assessed with X-ray. The bone cement leakage, pulmonary embolism, incidence of nearby vertebral fractures and other complications were evaluated during follow-up.
    RESULTS AND CONCLUSION: All patients were followed up for 12-18 months. The anterior vertebral height of the fractured vertebrae, the lower back pain and function, and quality of life were improved significantly after treatment (P < 0.05). All patients got the same neurological symptoms before surgery. The bone cement  dispersion was good after treatment, detected by X-ray and CT scan, only two cases appeared with bone cement leakage, but no clinical symptoms were found. There was no cement toxicity or allergic complications, pulmonary embolism, infection, nerve injury or new fractures. The high viscosity bone cement used in the treatment of osteoporotic thoracolumbar vertebral compression fractures can significantly relieve thoracic back pain, improve lower back function and quality of life, and greatly reduce the risk of bone cement leakage.


    中国组织工程研究杂志出版内容重点:生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程


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    Platelet-rich plasma combined with chondrocytes for construction of injectable tissue-engineered cartilage
    Wu Jun, Zhang Jun, Liu Jin-bo
    2014, 18 (30):  4764-4770.  doi: 10.3969/j.issn.2095-4344.2014.30.002
    Abstract ( 295 )   PDF (726KB) ( 452 )   Save

    BACKGROUND: Owing to containing large amounts of growth factors, platelet-rich plasma has been widely used in bone regeneration, wound healing, and so on, while few studies have been reported on cartilage tissue engineering.
    OBJECTIVE: To explore the effects of platelet-rich plasma on the proliferation and differentiation of chondrocytes, and the feasibility of constructing tissue-engineered cartilage by the combination of chondrocytes and platelet-rich plasma.
    METHODS: We detected the concentrations of transforming growth factor-β, insulin-like growth factor-1, platelet-derived growth factor and epidermal growth factor BB in the whole blood, platelet-rich plasma, and activated platelet-rich plasma. The rabbit articular chondrocytes were cultured in 10%, 15%, 20%, 30% platelet-rich plasma for 7 days, then the cell proliferation was tested by cell counting kit-8, and cartilage-related genes (collagen type II, Aggrecan, Sox-9) were determined using QT-PCR. The chondrocytes/platelet-rich plasma composite was implanted subcutaneously into the rabbits, and the samples were harvested after 6 weeks of transplantation for histological examination.
    RESULTS AND CONCLUSION: The levels of different growth factors in the platelet-rich plasma were higher than those in the whole blood (P < 0.05), but lower than those in the activated platelet-rich plasma (P < 0.05). Platelet-rich plasma at different concentrations promoted the proliferation of chondrocytes. When the 
    concentration of platelet-rich plasma was no more than 20%, the proliferation of chondrocytes showed a concentration-dependent manner. The 20% platelet-rich plasma showed the best effects to promote the expression of collagen type II (P < 0.05), while the 15% platelet-rich plasma could maximize the expression of Sox-9 and Aggrecan  (P < 0.05). After transplantation of chondrocytes/platelet-rich plasma composite, cartilage-like tissue formed with cartilage lacuna-like structures, and rich extracellular matrix was found, which indicates platelet-rich plasma can be used as an injective scaffold in cartilage tissue engineering.


    中国组织工程研究杂志出版内容重点:生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程


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    Preparation of tissue-engineered esophageal scaffolds using electrospinning technology
    Yu Xue-chan, Shen Qiu-xia, Lu Zhen-zhen, Zhang Chen, Deng Ling, Hu Pin, Zhu Ya-bin
    2014, 18 (30):  4771-4776.  doi: 10.3969/j.issn.2095-4344.2014.30.003
    Abstract ( 337 )   PDF (778KB) ( 671 )   Save

    BACKGROUND: We have found that oriented fibers can guide the alignment of smooth muscle cells in our previous experiments. Thus, we designed the experiment to prepare well aligned polymeric fibers using electrospinning technology, aiming at guiding the growth of esophageal smooth muscle cells to maintain cell morphology and biological function.
    OBJECTIVE: Using electrospinning technology, to fabricate isotropic and directed nano-fibrous scaffolds made of polycaprolacton, gelatin and silk fibroin.
    METHODS: Polycaprolacton/silk fibroin fibers at a ratio of 4:1 were prepared with proper parameters, including solution concentration, voltage and injection speed, under the self-made spinning system. The polycaprolacton/ gelatin sheets with mass ratio of 2:1, 1:1 and 1:2, respectively, were also fabricated under suitable process parameters. Using the roller collector instead of the metal plate, polycaprolacton/gelatin nano-fibrous scaffold with good alignment of fibers was manufactured.
    RESULTS AND CONCLUSION: The isotropic polycaprolacton/silk fibroin scaffold with fiber diameter of (535.9±126.7) nm was prepared under conditions of solution concentration (0.08 g/mL), injection speed (1.6 mL/h) and voltage (22.5 kV), and these fibers were uniform with no beads. The isotropic polycaprolacton/gelatin scaffold with fiber diameter of (257.9±117.8) nm was prepared under conditions of solution concentration (0.10 g/mL), injection speed (0.8 mL/h) and voltage (22.5 kV). Using the roller collector instead of the previous metal plate, polycaprolacton/ gelatin (w:w, 1:2) nano-fibrous scaffold with good alignment of fibers was manufactured. The process parameters were  3 000 r/min of rolling speed, 0.8 mL/h of injection speed and 15 kV of voltage.


    中国组织工程研究杂志出版内容重点:生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程


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    Three-dimensional finite element analysis of severe wedge-shaped defective premolar restored with fiber post and composite resin
    Shen Qing-yi, Wang Dong-mei, Zhong Qun, Chen Dong
    2014, 18 (30):  4777-4782.  doi: 10.3969/j.issn.2095-4344.2014.30.004
    Abstract ( 383 )   PDF (691KB) ( 498 )   Save

    BACKGROUND: Someone regards that treatments such as root canal therapy and post-core restoration can increase the fracture probability of teeth. Can fiber reinforced composite post enhance the fracture resistance of the severe wedge-shaped defected premolar? Three-dimensional finite element can simplify complex dental systems into a model analyzed by computer, and conduct biomechanical research through the model analysis.
    OBJECTIVE: To analyze the stress distribution of sever wedge-shaped defected first premolar which restored by fiber reinforced composite post and composite resin.
    METHODS: We established the three-dimensional finite element models of severe wedge-shaped defected maxillary first premolar before and after restoration with fiber reinforced composite post and composite resin. The maximum bite force was designed to 267 N that was applied at 45° angle. Then, we analyzed the distribution of maximum principal stress in finite element models.
    RESULTS AND CONCLUSION: The maximum principal stress was concentrated in the tip of defected region. After restoration, the distribution of maximum principal stress was similar to that of the normal premolar, and the tensile stress was distributed uniformly in the upper-central part of buccal root. It may be beneficial for improving fracture resistance ability of severe wedge-shaped-defect premolar by restoration with fiber reinforced composite post and composite resin.


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    Effect of glutaraldehyde cross-linking on the properties of chitosan/hydroxyapatite- gentamicin delayed materials
    Zhao Hong-xia
    2014, 18 (30):  4783-4789.  doi: 10.3969/j.issn.2095-4344.2014.30.005
    Abstract ( 291 )   PDF (2608KB) ( 703 )   Save

    BACKGROUND: Cross-linking is a common method for the modification of bone tissue engineering materials, but there are few studies about the effect of cross-linking on properties of drug-loaded artificial bone.
    OBJECTIVE: To study the effect of glutaraldehyde cross-linking on mechanical strength, degradation rate and in vitro release behavior of chitosan/hydroxyapatite-gentamicin delayed materials.
    METHODS: The chitosan/hydroxyapatite-gentamicin drug-loaded artificial bone and the cross-linked chitosan/hydroxyapatite-gentamicin drug-loaded artificial bone were prepared at 10%, 20% and 30% chitosan mass ratio. The mechanical strength, absorption rate, degradation rate and in vitro release behavior of materials in each group were determined.
    RESULTS AND CONCLUSION: The compressive strength of 10%, 20%, 30% chitosan/hydroxyapatite- gentamicin drug-loaded artificial bone was (10.16±1.17) MPa, (28.4±0.64) MPa and (23.28±1.30) MPa, respectively. After cross-linking, the strength was increased to (36.3±1.20) MPa, (51.6±2.08) MPa and  (36.9±3.22) MPa, respectively. The absorption rate and degeneration rate were reduced by cross-linking. In the first day of drug release in vitro, the rate of gentamicin released from non-cross-linked 30% chitosan/hydroxyapatite-gentamicin drug-loaded artificial bone was 42.2%, while the rate was decreased to 33.6% after cross-linking. At the following 9 days, the total release of the cross-linked material was lower than that of non-cross-linked material. Glutaraldehyde cross-linking could improve the stability, reduce the degradation rate, and significantly ameliorate the release of artificial bone.


    中国组织工程研究杂志出版内容重点:生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程


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    The inductive effect of microcapsuled chondrocytes cocultured with bone marrow mesenchymal stem cells
    Zhang Jin, Zhang Zi-qi, Zhang Yan, Xu Min
    2014, 18 (30):  4790-4796.  doi: 10.3969/j.issn.2095-4344.2014.30.006
    Abstract ( 331 )   PDF (2646KB) ( 351 )   Save

    BACKGROUND: Traditional coculture methods for directional differentiation of bone marrow mesenchymal stem cells, such as direct contact method and Transwell coculture system, appear to have low purity and slow proliferation.
    OBJECTIVE: To compare the inductive effect of microcapsule coculture system and traditional transwell coculture system on the differentiation of bone marrow mesenchymal stem cells. 
    METHODS: The passage 2 microcapsuled chondrocytes and the passage 3 bone marrow mesenchymal stem cells harvested from rabbits were co-cultured at a ratio of 1:1 in a Transwell chamber. Another passage 2 chondrocytes and passage 3 bone marrow mesenchymal stem cells were co-cultured using  traditional transwell coculture system. Pure bone marrow mesenchymal stem cells were served as controls. MTT assay was used to compare cell proliferation, toluidine blue staining and safranine O staining were used for observation of cartilage matrix synthesis, alcian blue staining and ELISA test were used to measure glycosaminoglycans and synthesis of type II collagen, respectively.
    RESULTS AND CONCLUSION: Compared with the traditional co-culture method, the microcapsule coculture system and pure culture method showed better cell proliferation (P < 0.05). The levels of glycosaminoglycans and type II collagen were higher in the microcapsule coculture group than the traditional coculture group and pure culture group (P < 0.05). Moreover, the microcapsule coculture group showed better outcomes in toluidine blue staining and safranine O staining than the traditional coculture group and pure culture group. These findings indicate that the microcapsule coculture system is more effective in the induction of bone marrow mesenchymal stem cells than traditional Transwell coculture system.


    中国组织工程研究杂志出版内容重点:生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程


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    Effect of Bio-Gide collagen membranes on the proliferation and osteogenetic differentiation of rabbit bone marrow mesenchymal stem cells
    Xu Fan, Yang De-sheng
    2014, 18 (30):  4797-4803.  doi: 10.3969/j.issn.2095-4344.2014.30.007
    Abstract ( 631 )   PDF (2445KB) ( 526 )   Save

    BACKGROUND: Bio-Gide collagen membranes show a good biocompatibility with stem cells. But the research on the osteogenetic differentiation of bone marrow mesenchymal stem cells cultured on the Bio-Gide collagen membranes is rarely reported.
    OBJECTIVE: To observe the effect of Bio-Gide collagen membranes on the proliferation and the osteogenetic differentiation of bone marrow mesenchymal stem cells.
    METHODS: Bone marrow mesenchymal stem cells from rabbits were isolated and cultured by using the whole bone marrow adherence method in vitro. Passage 3 bone marrow mesenchymal stem cells were selected and seeded on the Bio-Gide collagen membrane pretreated petri dish (experimental group) and simple petri dish (control group). The proliferation of bone marrow mesenchymal stem cells was detected by Cell Counting Kit-8 at 1, 4, 7, 14 days. The supernatant of the cells cultured in osteogenic differentiation medium were collected to detect the activity of alkaline phosphatase at 1, 4, 7, 14 days.
    RESULTS AND CONCLUSION: The number of bone marrow mesenchymal stem cells in the two groups was increased with the increasing time, and the control group had more cells than the experimental group at 7 days  (P < 0.05). There was no significant difference between the two groups at other time points. The alkaline phosphatase activity was increased with the increasing culture time, and the experimental group had a higher activity than the control group at 14 days (P < 0.05). There was no significant difference between the two groups at other time points. Experimental findings indicate that Bio-Gide collagen membranes can promote the proliferation and the osteogenetic differentiation of bone marrow mesenchymal stem cells.


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    Effect of the CAD/CAM zirconia all-ceramic crown restoration on periodontal tissue
    Shang Li-juan, Wu Yan, Xu Yong-jun
    2014, 18 (30):  4804-4809.  doi: 10.3969/j.issn.2095-4344.2014.30.008
    Abstract ( 916 )   PDF (757KB) ( 533 )   Save

    BACKGROUND: To maintain the long-term effect of full crown largely depends on the health of periodontal tissues.
    OBJECTIVE: To explore the effect of CAD/CAM zirconia all-ceramic crown restoration on the state of periodontal health.
    METHODS: Sixty-four abutments of 55 patients were randomly divided into two groups: the experimental group included 32 abutments of 29 patients which would be restored by CAD/CAM zirconia all-ceramic crowns; the controlled group included 32 abutments of 26 patients which would be restored by Ni-Cr alloy porcelain-fused-to- metal restorations. The volume of gingival crevicular fluid and the levels of interleukin-6 and tumor necrosis factor-α in the two groups were examined at the pre-restoration and post-restoration stages. Meanwhile, periodontal clinical indicators, including sulcus bleeding index, probing depth, plaque index and attachment loss were recorded.
    RESULTS AND CONCLUSION:
    No difference in various indexes was found in the experimental group before and after restoration (P > 0.05). At 12 months after restoration, in the control group, the volume of gingival crevicular fluid, the levels of interleukin-6 and tumor necrosis factor-α, sulcus bleeding index, probing depth, and plaque index were all increased (P > 0.05); meanwhile, these indexes in the experimental group were significantly lower than those in the control group (P < 0.05). Experimental findings suggest that the CAD/CAM zirconia all-ceramic crown restoration is more favorable to the health of periodontal tissues.


    中国组织工程研究杂志出版内容重点:生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程


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    Effect of different polypeptide modification methods on properties of micro-arc oxidation coatings covering pure titanium
    Wang Lei, Yan Feng-ying, Chen Jian-zhi, Wang Shou-biao
    2014, 18 (30):  4810-4815.  doi: 10.3969/j.issn.2095-4344.2014.30.009
    Abstract ( 519 )   PDF (764KB) ( 448 )   Save

    BACKGROUND: It can be theoretically speculated that the combination of titanium, micro-arc oxidation coating and arginine-glycin-aspartic acid (RGD) polypeptide will show better mechanical and biological properties.
    OBJECTIVE: To observe the microstructure and cell proliferation of the titanium and micro-arc oxidation coatings modified with RGD polypeptide by different modification methods.
    METHODS: Ninety specimens of pure titanium and micro-arc oxidation coatings were divided into three groups, with 30 specimens in each group. The first 30 specimens were pure titanium physically decorated with RGD polypeptide only. The other two groups of specimens were physical and chemical coupling adsorption RGD  polypeptide micro-arc oxidation samples, respectively. The fluorescence microscope was used to observe the effects and amounts of grafting RGD polypeptide on each sample. Content of the RGD polypeptide on the surface of the specimens were measured by X-ray photoelectron spectroscopy. The mouse bone marrow stromal cells were cultured on the surfaces of three groups samples, and the adhesion and proliferation of the cells cultured for 3 hours, 12 hours, 24 hours and 3 days were observed by optical microscope respectively.
    RESULTS AND CONCLUSION: There were green fluorescence spots, with varying size and amount, on the surface of specimens in three groups. In the unit field of view, the fluorescence was the strongest in chemical coupling adsorption RGD polypeptide micro-arc oxidation samples, indicating that these specimens were grafted with many polypeptides. In physical coupling adsorption RGD polypeptide micro-arc oxidation samples, small amount of polypeptides were found on the surface, and the content of the RGD polypeptide was the highest in chemical coupling adsorption RGD polypeptide micro-arc oxidation samples. No apparent cytotoxicity was observed in three groups. The cell proliferation was the best in chemical coupling adsorption RGD polypeptide micro-arc oxidation samples. Experimental findings suggest that, chemical coupling method can well fix the RGD polypeptides on the surface of pure titanium samples containing micro-arc oxidation, without any cytotoxicity, which contribute to promote the cell growth and proliferation.


    中国组织工程研究杂志出版内容重点:生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程


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    Influence of beryllium ions on genotype of Porphyromonas gingivalis fimbriae gene cluster
    Huang Rui, Li Qing-yan, Lin Hua, Guo Wei, Gao Ning
    2014, 18 (30):  4816-4822.  doi: 10.3969/j.issn.2095-4344.2014.30.010
    Abstract ( 297 )   PDF (1852KB) ( 375 )   Save

    BACKGROUND: In oral warm and moisture circumstance, alloy which contains Be is easily to be eroded to release Be2+. But there is still no research focusing on beryllium influence on genotype of Porphyromonas gingivalis fimbriae gene cluster.
    OBJECTIVE: To investigate Be2+ effect on genotype of Porphyromonas gingivalis fimbriae gene cluster.
    METHODS: The revived Porphyromonas gingivalis was resuscitated for 48 hours in the anaerobic culture medium with different concentration of Be2+ (10×10-6, 5×10-6, 1.25×10-6). Through PCR amplification and sequencing, we investigated the effects of Be2+ on genotypes of Porphyromonas gingivalis fimbriae gene cluster.
    RESULTS AND CONCLUSION: (1) When Be2+ concentration was 5×10-6, we found the peak of 217 and 257 sites on DNA sequence expressing G/A overlap peak, different from G single peak of the other groups, suggesting the suspicious bases changes, part of the single base G mutated into A. (2) On all concentrations, we found a base group composed of seven A bases was inserted into the 101 site of DNA sequence. Up to now, there is no direct contacts of the mutations occurring to Be2+ concentration. Changes of gene may lead to the shifting of the reading frame, the abnormal synthesis of proteins, the change of Porphyromonas gingivalis fimA gene toxicity, and lastly the unbalance of the micro-ecological environment.


    中国组织工程研究杂志出版内容重点:生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程


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    Preparation of amphiphilic superparamagnetic composite particles with tumor targeted MRI contrast agent
    Gu Jun-heng, Zhang Qing-yun, Zhang Wei, Yang Xin-lin
    2014, 18 (30):  4823-4830.  doi: 10.3969/j.issn.2095-4344.2014.30.011
    Abstract ( 278 )   PDF (508KB) ( 452 )   Save

    BACKGROUND: Superparamagnetic iron oxide nanoparticles (Fe3O4 NPs) have been widely used in MRI. It is vital to prepare the superparamagnetic MRI contrast agent with high stability, biocompatibility and tumor targeting in order to prevent the aggregation of Fe3O4 NPs and realize the high-precision diagnose of tumor.
    OBJECTIVE: To prepare the amphiphilic superparamagnetic composite particles with tumor targeting mediated by folate receptor.
    METHODS: The stable amphiphilic superparamagnetic composite particles with tumor targeting function were prepared by coating the Fe3O4 NPs with a Pluronic F127-folic acid conjugate, which was synthesized via an esterification reaction between the carboxyl group of the tumor targeting molecule, folic acid and the hydroxyl group of an amphiphilic triblock copolymer, Pluronic F127. The resultant Pluronic F127-folic acid-Fe3O4 composite particles were characterized by transmission electron microscopy, Fourier transform infrared-spectra, UV-vis absorption spectra, thermal gravimetric analysis, vibrating sample magnetometer and T2-weighted imaging. WST assay was used to characterize their cytotoxicity preliminarily.
    RESULTS AND CONCLUSION: The Pluronic F127-folic acid conjugates were prepared via esterification reaction. Then Fe3O4 NPs were wrapped with Pluronic F127-folic acid to result in the superparamagnetic composite particles with well dispersion and biocompatibility. The size of most superparamagnetic composite particles was less than 200 nm and the size of Fe3O4 core was 10-20 nm from the observation of transmission electron microscopy. The results from the Fourier transform infrared-spectra and UV-vis absorption spectroscop confirmed that folic acid molecules were modified on the surface of the superparamagnetic composite particles successfully. The mass ratio of Pluronic F127-folic acid conjugate was determined by thermal gravimetric analysis as 27.2 wt% in the resultant Pluronic F127-folic acid-Fe3O4 composite particles. The saturated magnetic intensity of the superparamagnetic composite particles was 47.35 emu/g by vibrating sample magnetometer and the relaxation rate was 0.025×106 mol/s from MRI. The WST assay showed the negligible cell cytotoxicity of Pluronic F127-folic acid-Fe3O4. The superparamagnetic composite particles have potential application as the MRI contrast agents with tumor targeting, and the Pluronic F127-folic acid-Fe3O4 composite particles is expected to be used as a MRI contrast agent for tumor targeting.


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    Mechanical and biological properties of porous calcium phosphate cement/fibrin glue for bone regeneration in vitro
    Dong Jing-jing, Bi Long, Li Na, Guo Yu, Xu Qiang, Lei Wei, Cui Geng
    2014, 18 (30):  4831-4837.  doi: 10.3969/j.issn.2095-4344.2014.30.012
    Abstract ( 476 )   PDF (2238KB) ( 470 )   Save

    BACKGROUND: Fibrin glue introduced into calcium phosphate cement has not been confirmed whether this way could overcome the compressive limits and the low degradation of calcium phosphate cement and to modify the biological properties of calcium phosphate cement.
    OBJECTIVE: To explore the mechanical and biological properties of calcium phosphate cement/fibrin glue at different powder/liquid ratio for bone regeneration in vitro.
    METHODS: Calcium phosphate cement and fibrin glue were mixed at ratios of 1:1, 3:1, 5:1 (mL/g), and the pure calcium phosphate cement served as controls. Setting time, scanning electron microscope and the biomechanical test were used to analyze the composite scaffold structure, physical performance and the mechanical properties. Passage 3 osteoblasts were respectively inoculated on the material surface of the four groups, and pure cells served as blank controls. Cell adhesion, proliferation and alkaline phosphatase activity were observed.
    RESULTS AND CONCLUSION: The initial and final setting time of calcium phosphate cement/fibrin glue at 1:1 and 3:1 (mL/g) was higher than that in the control group (P < 0.05), while the initial and final setting time of calcium phosphate cement/fibrin glue at 5:1 (mL/g) was lower than that of the control group ( < 0.05). Scanning electron microscope showed smoother and denser surface of composite scaffolds compared with the pure calcium phosphate cement. The aperture of the composite scaffolds was decreased with the increasing concentration of fibrin glue. The compressive strength of composite scaffolds at 3:1 and 5:1 was higher than that of the control group (P < 0.05), while the modulus of the composite scaffolds at 1:1, 3:1, 5:1 was higher than that of the control group ( < 0.05). Cell adhesion, proliferation and alkaline phosphatase activity showed no difference among the three composite scaffold and control groups, but all higher than the blank control group ( < 0.05). These findings indicate that fibrin glue introduced into calcium phosphate cement can overcome the low-strength limits of calcium phosphate cement, and maintain the good biological properties of calcium phosphate cement for bone regeneration.


    中国组织工程研究杂志出版内容重点:生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程


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    Effect of titanium dioxide nanoparticles on mechanical strength and bacterial inhibition of glass ionomer cements
    Liu Li-xia, Chen Lin
    2014, 18 (30):  4838-4844.  doi: 10.3969/j.issn.2095-4344.2014.30.013
    Abstract ( 373 )   PDF (2564KB) ( 506 )   Save

    BACKGROUND: Glass ionomer cements have been gradually employed in many aspects of dental clinical field. However, low mechanical strength and antibacterial effect restrict its further applications.
    OBJECTIVE: To proportionally explore the effects of titanium dioxide nanoparticles on the mechanical strength and antibacterial effect of traditional glass ionomer cements.
    METHODS: Base on different mass fractions of titanium dioxide nanoparticles in glass ionomer cements, all the glass ionomer cement samples were divided into control group (no titanium dioxide nanoparticles), low titanium dioxide group (containing 3% titanium dioxide nanoparticles), medium titanium dioxide group (containing 6% titanium dioxide nanoparticles), and high titanium dioxide group (containing 9% titanium dioxide nanoparticles). A universal material testing machine and a hardness tester were used to examine flexural strength, compressive strength, and surface hardness of glass ionomer cement samples, respectively. Glass ionomer cement samples were immerged into the artificial saliva, and fluoride release was measured by using a fluoride ion selective electrode. The direct contact test was used to investigate antibacterial effect of glass ionomer cement samples towards Streptococcus mutans.
    RESULTS AND CONCLUSION: Compared with the control group, few titanium dioxide nanoparticles (low and medium titanium dioxide group) could significantly improve flexural strength, compressive strength and surface hardness of glass ionomer cement samples (P < 0.05), and high titanium dioxide nanoparticles (high titanium dioxide group) significantly decreased flexural strength, compressive strength and surface hardness (P < 0.05). The introduction of titanium dioxide nanoparticles had little effect on fluorine release behavior of glass ionomer cement samples, and greatly improved antibacterial effect of glass ionomer cement samples towards Streptococcus mutans.


    中国组织工程研究杂志出版内容重点:生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程


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    Effect of type I or type II collagen on biological characteristics of human chondrocytes
    Jiang Ping, Wei Peng, Zhao Ming-cai, Chen Qiong, Wang Zi
    2014, 18 (30):  4845-4850.  doi: 10.3969/j.issn.2095-4344.2014.30.014
    Abstract ( 661 )   PDF (1587KB) ( 572 )   Save

    BACKGROUND: Experiments have shown that the collagen substrate has the capability of stimulating cartilage generation, but the stimulating role of different types of collagen substrates remains controversial.
    OBJECTIVE: To investigate the effect of type I and type II collagen on the biological characteristics of human chondrocytes cultured in vitro.
    METHODS: Human chondrocytes at passage were cultured onto the ordinary culture plates (ordinary plate), type I collagen-coated culture plates (type I plate), and type II collagen-coated culture plates (type II plate). Cell growth curves were determined by MTT method after cells were cultured for 10 days. By ELISA, PCR, and 1,9-dimethyl methyleneblue technology, type I and type II collagen and glycosaminoglycan contents were quantitatively detected in cartilage cells 28 days after culture.
    RESULTS AND CONCLUSION: The number of cartilage cells was the highest in type II plate, which was twice of that in type I plate and five times of that in ordinary plate. Cartilage cells in type II plate secreted the least amount of type I collagen, which showed significant differences compared with the ordinary plate (P < 0.01) and had no statistically significant difference with type I plate (P > 0.01). Cartilage cells in type II plate secreted the most amount of type II collagen and glycosaminoglycan, showing significant differences compared with the other two plates (P < 0.01). The cartilage cells cultured in collagen plates are better than that cultured in ordinary culture plate, type II collagen culture plate is better than type I collagen culture plate in maintaining cell shape, extending the dedifferentiation pattern, and promoting cell differentiation.


    中国组织工程研究杂志出版内容重点:生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程


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    Sustained release of polyethylene glycol-modified hydrogel-packaged growth factors and endothelial progenitor cells
    Han Yan-jiu, Liu Guo-hui, Ouyang Liu
    2014, 18 (30):  4851-4856.  doi: 10.3969/j.issn.2095-4344.2014.30.015
    Abstract ( 255 )   PDF (805KB) ( 380 )   Save

    BACKGROUND: Many studies have demonstrated that in order to achieve the endothelialization of the polymer materials, surface loading of bioactive factors is an important means and the introduction of human endothelial cells on the surface of the polymer materials will contribute to increase the biocompatibility of materials.
    OBJECTIVE: To synthesize the polyethylene glycol hydrogel packaging vascular endothelial growth factor, basic fibroblast growth factor and endothelial progenitor cells, and to observe the sustained release of growth factors and culture status of endothelial progenitor cells.
    METHODS: The polyethylene glycol-modified hydrogel containing arginine-glycine-aspartic acid peptide was synthesized, and then, rat endothelial progenitor cells, basic fibroblast growth factor, vascular endothelial growth factor, matrix metalloproteinases were successively added. The hydrogel were immersed in PBS, and ELISA was used to test the levels of growth factors in supernatants every 12 hours. Matrix metalloproteinases-2 (100, 1 000 ng) and matrix metalloproteinases-9 (100, 1 000 ng) at different mass were added into the PBS containing hydrogel after 72 hours. ELISA was also used to test the levels of growth factors in supernatants every 12 hours. The hydrogel encapsulating endothelial progenitor cells was cultured in a culture medium for 5 days, and the number of survival cells was counted by a flow cytometer after digestion.
    RESULTS AND CONCLUSION: Within 12-72 hours, the release percentage of basic fibroblast growth factor and vascular endothelial growth factor remained at about 41%. After 72 hours, the release percentage of both growth factors was found to grow steadily when matrix metalloproteinases-2 (100, 1000 ng) and matrix metalloproteinases-9 (100, 1 000 ng) at different mass were added, which reached 95%. The release percentage was increased with the increasing mass of matrix metalloproteinases. After 5 days of culture, 88.17% cells still survived in the hydrogel. These findings indicate that sell-assembly polyethylene glycol-modified hydrogel can realize the controlled release of basic fibroblast growth factor and vascular endothelial growth factor, and it can also support the proliferative growth of endothelial progenitor cells.


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    Optimization research on preparation conditions of polymer-shelled microbubble contrast agents
    Huang Hong-jie, Lin Peng, Huang Rong-sen, Lin Yi-kai, Su Jie, Chen Wan-ping, Lin Li-wu, Xue En-sheng, Chen Zhi-kui
    2014, 18 (30):  4857-4862.  doi: 10.3969/j.issn.2095-4344.2014.30.016
    Abstract ( 431 )   PDF (903KB) ( 570 )   Save

    BACKGROUND: In previous experiments, a polymer-shelled microbubble contrast agent was successfully prepared using L-polylactic acid-polyethylene glycol methyl-covered liquid fluorocarbon perfluoropentane, which developed well in vivo and in vitro under a second harmonic contrast-enhanced ultrasound with a low mechanical index
    OBJECTIVE: To optimize the conditions for preparing polymer-shelled microbubble contrast agents with high yield and suitable size.
    METHODS: The polymer-shelled microbubble contrast agent containing perfluoropentane was prepared by single emulsion method. Three preparation conditions, including polymer mass/perfluoropentane volume ratio (4/1, 2/1, 1/1, 1/2), hemogenate speed (18 000, 26 000, 35 000 r/min) and time (15, 30, 60, 120 seconds), were optimized, and the microbubble yield and size were set as judge standard. The contrast-enhanced ultrasonography of rabbit kidney was performed using the optimized microbubbles, and the arrival time, time to peak, half time of descending and peak intensity were analyzed by TCA software.
    RESULTS AND CONCLUSION: The preparation conditions were optimized as a polymer mass/perfluoropentane volume ratio of 2/1, homogenate speed of 26 000 r/min and homogenate time of 60 seconds, which presented a high microbubble yield of (1.8±0.4)×109/mL and mean size of (3.7±1.3) μm with uniform distribution. The arrival time, time to peak, half time of descending and peak intensity were detected as (3.1±0.6) seconds, (2.9±0.5) seconds, (4.0±0.7) seconds and (4.7±1.1)×10-5 AU, respectively. The polymeric microbubbles prepared by optimized conditions were of high yield, with suitable size and good contrast-enhanced effect in vivo.


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    Effect of different decellularizing methods on cell-secreted extracellular matrix in vitro
    Fan Li-mei, Xia Rong, Dou Xiao-chen
    2014, 18 (30):  4863-4867.  doi: 10.3969/j.issn.2095-4344.2014.30.017
    Abstract ( 311 )   PDF (660KB) ( 394 )   Save

    BACKGROUND: Compared with single or composite biomolecular materials, decellulazied matrices are more biomimetic to natural ectocytic surroundings. So cell-secreted extracellular matrix is paid more and more attention in the field of tissue engineering, and the composition of these matrices are influenced by decellulezired preparation methods more or less. But there are few studies about the biological effect of different decellulazried methods on the extracellular matrix.
    OBJECTIVE: To investigate the composition of cell-secreted extracellular matrices in vitro by different decellularizing methods and their effects as surface modification on cytobiological reaction.
    METHODS: After the treatment of different decellularizing methods (freeze/thaw cycles, trypsin, weak alkal, detergents), the extracellular matrix was obtained and grouped into four kinds. The biological composition of the extracellular matrix was determined by ELISA assay. Then osteoblasts were seeded onto the four kinds of extracellular matrix surfaces. Cells cultured normally served as controls. The effect of extracellular matrix coatings on cell growth and differentiation were determined by MTT test and alkaline phosphatase activity test.
    RESULTS AND CONCLUSION: The residual components were the most in the freeze-thaw group, followed by the detergent and weak alkal groups, and the least in the trypsin group. Compared with the control group, the absorbance value of cells were lower in the freeze-thaw and detergent groups at days 3, 5, 7 after inoculation (both P < 0.05); the alkaline phosphatase activity was lower in the trypsin group at days 5, 7 after inoculation (P < 0.05), in the weak alkal group at 7 days after inoculation (P < 0.05), and in the detergent group at days 3, 5, 7 after inoculation (P < 0.05). Therefore, we can harvest more extracellular matrices by the freeze-thaw method, and the extracellular matrix coating synthesized by the freeze-thaw method is more helpful for cell growth than others.


    中国组织工程研究杂志出版内容重点:生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程


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    Ultrasonic controlling of degradation of polymer materials
    Gao Xi-xiang, Zhang Jian, Chen Bing, Gu Yong-quan, Li Jian-xin, Zhang Shu-wen, Ye Lin, Feng Zeng-guo
    2014, 18 (30):  4868-4872.  doi: 10.3969/j.issn.2095-4344.2014.30.018
    Abstract ( 341 )   PDF (753KB) ( 655 )   Save

    BACKGROUND: Degradable polymer materials initiate the degradation process immediately after implantation. How to regulate the degradation of these materials is rarely reported at present.
    OBJECTIVE: To study the effect of ultrasonic wave on controlling the degradation of polymer materials.
    METHODS: The sample is made of ε-caprolactone/L-lactide copolymer, and its core was coated with low density polyethylene on the surface with the following four different methods. (1) The core surface was firstly covered with CaCl2 powder, and then coated with polyethylene. (2) The core was firstly coated with polyethylene and coarsened for 3 hours. (3) The core surface was firstly covered with CaCl2 powder, and then coated with polyethylene, and coarsened for 3 hours. (4) The core was directly coated with polyethylene. The four kinds of specimens obtained were embedded in pork for ultrasonic bombardment experiment in vitro.
    RESULTS AND CONCLUSION: In the specimens prepared with methods 1 and 4, the lyophobic layer could protect core materials before ultrasonic treatment, and no absorption peak was found at 631 nm. After ultrasonic treatment, the lyophobic layer was destroyed, toluidine blue dye was released, leading to change the color of immersion solution and increase the absorption peak at 631 nm. In the specimens prepared with methods 2 and 3, the lyophobic layer cannot exhibit the protection effects, the absorption peak was found at 631 nm. Under electron microscope, the appearance of the specimens in four groups was changed obviously. It is feasible to control the starting of the degradation by coating the degradable copolymer with LDPE and using ultrasonic as a trigger.


    中国组织工程研究杂志出版内容重点:生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程


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    Determination of fructose and glucose in assisted reproductive culture medium by high performance liquid chromatography-differential refractive index detector
    Sun Xue, Huang Yuan-li, Ke Lin-nan
    2014, 18 (30):  4873-4877.  doi: 10.3969/j.issn.2095-4344.2014.30.019
    Abstract ( 659 )   PDF (661KB) ( 599 )   Save

    BACKGROUND: Nowadays, the component content of assisted reproductive culture medium and their test methods are unclear in the quality standards. We need to establish the test methods to fill this vacancy, so as to control the quality of assisted reproductive culture medium effectively.
    OBJECTIVE: To establish an evaluation method for determination of fructose and glucose in assisted reproductive culture medium, thereby based on which to establish the quality standards.
    METHODS: High performance liquid chromatography-differential refractive index detector was adopted and Rezex RCM-Monosaccharide Ca2+ (8%) (300.0 mm×7.8 mm) column was used. The mobile phase was ultrapure water at the flow rate of 0.6 mL/min and the temperature of 80 ℃.
    RESULTS AND CONCLUSION: Resolution of the peaks of glucose and fructose was 3.2. The linear ranges of glucose and fructose were 30.1-502 mg/L (r=0.999 8) and 102-408 mg/L (r=0.999 8), respectively. The relative standard deviation of reproducibility test was 0.17% and 0.40%, respectively. The relative standard deviation of stability test was 0.22% and 0.73%, respectively. The glucose group and fructose group average recovery rates were 1.22% and 1.38%, respectively. The methodology of High performance liquid chromatography-differential refractive index detector accorded with the requirements. The glucose contents of samples 1 and 2 were 96.7 mg/L and 99.6 mg/L, respectively. The fructose contents of samples 1 and 2 were 208.5 mg/L and 197.4 mg/L, respectively. A reliable, simple, and accurate method was provided for the quality control of assisted reproductive culture medium, which fills the domestic vacancy in the quality standards for assisted reproductive culture medium.


    中国组织工程研究杂志出版内容重点:生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程


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    Design and application of biodegradable coronary stents: what will be brought by further innovations in materials science?
    Chen Jia-hui, Shen Li, Wang Qi-bing, Ge Jun-bo
    2014, 18 (30):  4878-4888.  doi: 10.3969/j.issn.2095-4344.2014.30.020
    Abstract ( 599 )   PDF (483KB) ( 1132 )   Save

    BACKGROUND: With the social and economic development and aging, coronary heart disease has become the primary cause of death endangering human life and health, while cardiovascular intervention (especially stenting) in the treatment of cardiovascular disease is at a growing status.
    OBJECTIVE: To describe the development, research progress and latest clinical application of heart interventional materials as well as advantages and disadvantages of biodegradable stents, and meanwhile to prospect for its future development and improvement.
    METHODS: A computer-based online search was conducted in PubMed for English language publications from January 1999 to April 2014 using the key words of “stent, scaffold, bioabsorbable, bioresorbable, biodegradable, biocompatibility, material properties” in English.
    RESULTS AND CONCLUSION: The new fully biodegradable stents are considered as another major progress in the cardiac intervention, which brings a new gospel for patients with coronary heart disease. In the initial stage of implantation, biodegradable stents exhibit the same mechanical support as bare metal stents; after implantation, anti-proliferative drugs are released to prevent thrombosis and restenosis, and the stent is completely degraded within a specified period, which significantly reduces the late-stage and very late-stage thrombosis and the risk of in-stent restenosis. However, long-term safety and efficacy of biodegradable stents need further studies. Innovation of stent design and materials science is the key to overcome the current shortcomings of biodegradable stents.


    中国组织工程研究杂志出版内容重点:生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程


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    Porous materials in tissue engineering
    Cong Yong-jian, Song Min
    2014, 18 (30):  4889-4894.  doi: 10.3969/j.issn.2095-4344.2014.30.021
    Abstract ( 823 )   PDF (606KB) ( 523 )   Save

    BACKGROUND: A favorable application prospect has been showed in basic and clinical research of porous material implantation in recent years.
    OBJECTIVE: To retrospect the progress in basic and clinical research of porous materials in the past 10 years and to summarize the existing problems and clinical prospects.
    METHODS: A computer-based search of PubMed and CNKI databases was performed for relevant articles using the keywords of “porous material, bone implantation” in English and Chinese, respectively. The included articles were related to porous materials and composite materials used in vitro, in vivo or in clinic, as well as porous materials closely related with bone defect repair or bone tissue engineering.
    RESULTS AND CONCLUSION: Hydroxyapatite, titamium and its alloys, polyethylene, calcium phosphates are porous materials that have been studied presently. Increasing experimental studies have shown that the porous materials have good histocompatibility and osteoinductive features in vitro and in vivo. But the uniform standards for porosity size in favor of tissue growth that is fastest and most effective have not been obtained. It is unclear how to gain and control the porosity of porous materials that are irregular, how to control the elasticity modulus, and how to gain 100% porosity effectively. Furthermore, whether the inflammation and exposure of porous materials in clinical application are related to the above-mentioned problems are unable to confirm.


    中国组织工程研究杂志出版内容重点:生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程


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    Development of bone tissue engineering scaffold materials
    Ma Xin-fang, Zhang Jing-ying
    2014, 18 (30):  4895-4899.  doi: 10.3969/j.issn.2095-4344.2014.30.022
    Abstract ( 677 )   PDF (599KB) ( 1313 )   Save

    BACKGROUND: Bone tissue engineering is the most promising way to treat bone defects at present. The key problem is to construct vascular networks which can provide oxygen and nutrients for new bone, and thereby provide a way for the body metabolism.
    OBJECTIVE: To review the characteristics of bone tissue engineering scaffold materials and to introduce the development of composite scaffold materials.
    METHODS: With the key words of “bone tissue engineering, scaffold, vascularization, composite scaffold” in Chinese and in English, respectively, a computer-based search was performed for articles published in CNKI and PubMed databases from January 2001 to January 2014. After the initial screening, the reserved articles were further detailed, summarized and concluded.
    RESULTS AND CONCLUSION: According to the different sources, the bone tissue engineering scaffold materials can be divided into artificial materials, natural derivatives and composite scaffold materials. Single scaffold is difficult to be the most ideal material for repair of bone defects, while composite scaffold can make up for the defects of the single scaffold to different degrees. Therefore, in recent years, the bone tissue engineering scaffolds have developed from single to composite scaffolds and there is the trend of organic combination of artificial materials and natural derivatives. However, composite scaffolds have many problems to be solved in the clinical application. The main aspect is to control the proportion of the composite scaffold so that the degradation of materials can be matched with growth of tissues and cells. The other one is to keep the porous and high mechanical strength of the composite scaffold.


    中国组织工程研究杂志出版内容重点:生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程


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    Extradural cortical stimulation for neural network recovery in stroke patients
    Zhao Jian-le, Li Jing-qi, Niu Sen-lin, Gao Jian
    2014, 18 (30):  4900-4905.  doi: 10.3969/j.issn.2095-4344.2014.30.023
    Abstract ( 430 )   PDF (605KB) ( 588 )   Save

    BACKGROUND: Extradural cortical stimulation combines the advantages of repetitive transcranial magnetic stimulation, transcranial direct current stimulation, subdural cortical stimulation and deep brain stimulation, which can significantly improve motor and language function after stroke.
    OBJECTIVE: To review the theoretical research and clinical application of extradural cortical stimulation for stroke recovery.
    METHODS: An online retrieval of PubMed database and CNKI database between January 1995 and April 2014 was performed for articles on theoretical research and clinical application of extradural cortical stimulation for stroke recovery, with the key words of “cortical stimulation, extradural motor cortex stimulation, extradural cortical implants, extradural cortical stimulation, stroke, rehabilitation” in English and Chinese.
    RESULTS AND CONCLUSION: Because of implantable cortical stimulation, the advantage of extradural cortical stimulation is its minimal invasiveness, high accuracy and transdural contact with the brain. For lack of effective treatment for the chronic phase of stroke patients with motor and language dysfunction, extradural cortical stimulation may be a new therapeutic method. Motor and language functional improvement must derive from reactivation of plasticity, local enhancement of perilesional areas, enhancement of network function and inter-hemispheric balance function, and amplification of sensory input.


    中国组织工程研究杂志出版内容重点:生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程


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    Predictive factors for in-stent late loss and non-culprit coronary lesion progression in diabetic patients undergoing drug eluting stenting
    Xie Yu-xia, Shi Xue-kun, Yang Jie, Wu Gang, Cheng Lu, Qiao Rui
    2014, 18 (30):  4906-4910.  doi: 10.3969/j.issn.2095-4344.2014.30.024
    Abstract ( 389 )   PDF (593KB) ( 350 )   Save

    BACKGROUND: Previous studies have suggested that the risks for coronary atherosclerotic plaque progression and in-stent restenosis are increased in patients with coronary heart disease combined with type 2 diabetes.  
    OBJECTIVE: To explore the predictive factors for in-stent late loss and non-culprit coronary lesion progression in patients with type 2 diabetes mellitus.
    METHODS: A total of 399 stenting patients were enrolled, including 179 diabetic patients and 220 non-diabetic patients. The clinical materials, angiography parameters and biochemical markers were collected. The difference between the two groups was compared, and also we conducted subgroup analysis in the diabetic patients. Low-density lipoprotein cholesterol, hemoglobin A1c, fibrinogen and high-sensitivity C-reactive protein were detected at days 3, 120, 210 and 360 after stenting.
    RESULTS AND CONCLUSION: Compared with non-diabetic patients, the stent length (P=0.18) was longer and the stent diameter (P=0.002) was smaller in the diabetic patients. The minimal lumen diameters of post-procedure and follow-up angiography in the diabetic group were significantly decreased (P=0.001, P=0), and the diabetic patients also showed severe coronary artery stenosis instantly and within the follow-up after stenting (P=0.038, P=0.004). The follow-up angiography showed that the diabetic patients had more late loss and restenosis (P=0, P=0.097). Furthermore, in the subgroup analysis of diabetic patients, the levels of hemoglobin A1c, fibrinogen and high-sensitivity C-reactive protein were significantly increased in the patients with restenosis and non-culprit lesion progression. These findings indicate that diabetic patients appear to have the higher incidence of restenosis and non-culprit lesion progression. Moreover, hemoglobin A1c, fibrinogen and high-sensitivity C-reactive protein are effective predictors for in-stent late loss and non-culprit coronary lesion progression.


    中国组织工程研究杂志出版内容重点:生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程


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    Polytetrafluoroethylene segment implantation for arteriovenous internal fistula with outflow tract obstruction
    Zhang Yu, Zhu Zhi-qiang, Zheng Xin, Ma Xue-tao, Liu Jian-wei, Han Zhi-you
    2014, 18 (30):  4911-4916.  doi: 10.3969/j.issn.2095-4344.2014.30.025
    Abstract ( 603 )   PDF (810KB) ( 425 )   Save

    BACKGROUND: Arteriovenous internal fistula is the first choice for hemodialysis. In the process of hemodialysis, many patients suffer from venous outflow stenosis. The methods including thrombolysis, intervention, surgical repair and fistula reconstruction all have their disadvantages.
    OBJECTIVE: To compare the midterm effects of polytetrafluoroethylene segment implantation and exclusively surgical repair in arteriovenous internal fistula with outflow tract obstruction.
    METHODS: Eighty patients with venous outflow stenosis, aged 22-80 years, were divided into test group (n=50; polytetrafluoroethylene segment implantation) and control group (n=30; simple surgical repair). The post-operative infection rate, postoperative time till recurrence of fistula dysfunction, and accumulate survival rate were compared between the two groups.
    RESULTS AND CONCLUSION: During the follow-up period of 10-28 months in the test group, there were nine patients with vascular access dysfunction, and the accumulate survival rate was 100% for 6 postoperative months, 92% for 12 months, and 82% for 18 months. In the control group, there were seven cases of vascular access dysfunction at 8-28 months of follow-up, and the accumulate survival rate was 93% for 6 postoperative months, 87% for 12 months, and 77% for 18 months. No statistically significant difference in the postoperative infection rate was observed between the two groups. The Kaplan-Meier survival curves showed that the accumulate survival rate was slightly higher in the test group than the control group, but there was no significant difference based on log-rank test (P=0.44). These findings indicate that polytetrafluoroethylene segment implantation for arteriovenous internal fistula with outflow tract obstruction has the similar effects as the surgical repair if it does not alter the autologous behavior of the initial access and maximally reserve the vessels for puncture.


    中国组织工程研究杂志出版内容重点:生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程


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    Vertebroplasty with artificial bone repairs vertebral compression fractures in the elderly: 3-month follow-up results
    Zhao Wei-biao, Li Ji, Li Yi, He Zi-wei
    2014, 18 (30):  4917-4920.  doi: 10.3969/j.issn.2095-4344.2014.30.026
    Abstract ( 378 )   PDF (580KB) ( 390 )   Save

    BACKGROUND: Bone cement used in the vertebroplasty treatment of osteoporotic vertebral compression fractures is nonabsorbable and has stronger intensity than normal bone tissue, which can cause further fractures of adjacent segments.
    OBJECTIVE: To investigate the short-term effect of vertebroplasty with artificial bone in the treatment of osteoporotic thoracolumbar fractures.
    METHODS: A total of 21 patients with osteoporotic vertebral compression fractures were enrolled, including 7 males and 14 females, aged 61-89 years old. All the patients were subjected to vertebroplasty with calcium sulfate injection. Visual analog scale and Oswestry Disability Index assessment were used before and at 0, 24, 48 weeks after treatment; meanwhile, X-ray and CT were used to observe the filling and leakage of artificial bone.
    RESULTS AND CONCLUSION: After treatment, all the patients showed improvements in the scores of visual analog scale and Oswestry Disability Index (P < 0.01). X-rays and CT scans showed that there were three cases of leakage but without spinal cord and nerve compressions. After 3 months, most of the artificial bone was absorbed. These findings indicate that vertebroplasty with artificial bone can ease pain and improve living conditions of patients with osteoporotic vertebral compression fractures.


    中国组织工程研究杂志出版内容重点:生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程


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