Effect of different polypeptide modification methods on properties of micro-arc oxidation coatings covering pure titanium

doi:10.3969/j.issn.2095-4344.2014.30.009

Abstract

Abstract:

BACKGROUND: It can be theoretically speculated that the combination of titanium, micro-arc oxidation coating and arginine-glycin-aspartic acid (RGD) polypeptide will show better mechanical and biological properties.
OBJECTIVE: To observe the microstructure and cell proliferation of the titanium and micro-arc oxidation coatings modified with RGD polypeptide by different modification methods.
METHODS: Ninety specimens of pure titanium and micro-arc oxidation coatings were divided into three groups, with 30 specimens in each group. The first 30 specimens were pure titanium physically decorated with RGD polypeptide only. The other two groups of specimens were physical and chemical coupling adsorption RGD polypeptide micro-arc oxidation samples, respectively. The fluorescence microscope was used to observe the effects and amounts of grafting RGD polypeptide on each sample. Content of the RGD polypeptide on the surface of the specimens were measured by X-ray photoelectron spectroscopy. The mouse bone marrow stromal cells were cultured on the surfaces of three groups samples, and the adhesion and proliferation of the cells cultured for 3 hours, 12 hours, 24 hours and 3 days were observed by optical microscope respectively.
RESULTS AND CONCLUSION: There were green fluorescence spots, with varying size and amount, on the surface of specimens in three groups. In the unit field of view, the fluorescence was the strongest in chemical coupling adsorption RGD polypeptide micro-arc oxidation samples, indicating that these specimens were grafted with many polypeptides. In physical coupling adsorption RGD polypeptide micro-arc oxidation samples, small amount of polypeptides were found on the surface, and the content of the RGD polypeptide was the highest in chemical coupling adsorption RGD polypeptide micro-arc oxidation samples. No apparent cytotoxicity was observed in three groups. The cell proliferation was the best in chemical coupling adsorption RGD polypeptide micro-arc oxidation samples. Experimental findings suggest that, chemical coupling method can well fix the RGD polypeptides on the surface of pure titanium samples containing micro-arc oxidation, without any cytotoxicity, which contribute to promote the cell growth and proliferation.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

全文链接：

Key words: titanium, peptides

CLC Number:

R318

Wang Lei, Yan Feng-ying, Chen Jian-zhi, Wang Shou-biao . Effect of different polypeptide modification methods on properties of micro-arc oxidation coatings covering pure titanium[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(30): 4810-4815.

Figures/Tables 2

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