Differentially expressed long non-coding RNAs in adipogenic differentiation of human adipose-derived stem cells

doi:10.3969/j.issn.2095-4344.2014.28.011

Abstract

Abstract:

BACKGROUND: The obesity has led to a plenty of diseases including hypertension, coronary heart disease, fatty liver, hyperlipidemia, and type 2 diabetes. Therefore, understanding the mechanism of adipocyte differentiation is of far-reaching significance to the prevention and treatment of obesity. For the current studies of the mechanism of adipocyte differentiation pay more attention to microRNA, rather than long non-coding RNAs (lncRNAs).
OBJECTIVE: To obtain the lncRNAs whose fold change was apparent during adipogenic differentiation, and to further screen the lncRNAs that possibly play a crucial role in adipogenic differentiation for verification.
METHODS: Subcutaneous fat was obtained from human abdomen. Adipose-derived stem cells were collected using tissue culture method. The third passage of adipose-derived stem cells was used for adipogenic differentiation. Through microarray technology, the expression levels of lncRNAs and mRNA were analyzed at 0, 5 and 12 days in adipogenic differentiation. Combining with bioinformatics report, lncRNAs apparently presented fold change were screened and verified by qRT-PCR.
RESULTS AND CONCLUSION: Fold change 1.5 (P < 0.05) was considered as a criterion during adipogenic differentiation. The number of up-regulated lncRNAs was 748 for 5 days versus 0 day, 847 for 12 days versus 0 days, 593 for 12 days versus 5 days. At the same time, the down-regulated number was 828 for 5 days versus 0 day, 1 113 for 12 days versus 0 day, 750 for 12 days versus 5 days during adipogenic differentiation. In
combination with bioinformatics analysis results, 3 of 28 lncRNAs were related to lipid metabolism: AK304548, BP216319 and DA852857, according to the standard that fold change in 0, 5 and 12 days was higher, and the target genes were known to be associated with adipogenesis-related genes. PCR results showed that the expression of AK304548 and BP216319 and its target gene presented an up-down trend, which is consistent with the microarray sequencing results. These results indicated that lncRNA plays a critical regulatory role in the adipogenic differentiation.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: intra-abdominal fat, mesenchymal stem cells, adipogenesis, RNA

CLC Number:

R394.2

Yue Wen-jun, Wang Xiang-mei, Zhang Qin, Huang Hai-hua, Wei You-wan, Peng Zhi. Differentially expressed long non-coding RNAs in adipogenic differentiation of human adipose-derived stem cells[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(28): 4491-4497.

Figures/Tables 10

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