Liver cancer stem cell isolation and biological characteristics

doi:10.3969/j.issn.2095-4344.2014.28.020

Abstract

Abstract:

BACKGROUND: Normal stem cells and tumor stem cells have differences in cell signaling pathway of gene expression and dependence. How to find a therapeutic method of selectively killing tumor stem cells is a topic that should be studied greatly.
OBJECTIVE: To isolate human liver cancer stem cells MHCC97 and to investigate the biological characteristics of them.
METHODS: Tumor stem cells were selected from highly metastatic human hepatoma cell line MHCC97 by flow cytometry. CD133^-CD34^-MHCC97 from normal human liver stem cells and CD133⁺CD34⁺ MHCC97 from tumor stem cells were isolated. Their phenotypes, growth curve, cell cycle and multi-lineage differentiation were measured.
RESULTS AND CONCLUSION: Phenotypes of CD133⁺CD34⁺ MHCC97 cancer stem cells were CD133⁺and CD34⁺ and they had the same growth curve and cell cycle with the liver stem cells CD133^-CD34^-MHCC97, besides, they had the differential ability to epithelial cells and endothelial cells that proved to be stem cells. These results indicated that human cancer stem cells CD133⁺CD34⁺ MHCC97 had the specific characteristics of tumor stem cells, could differentiate into epithelial cells and endothelial cells, had the biological property of tumor stem cells, was an origin of tumor relapse and metastasis, and a target of clinical therapy.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: stem cells, neoplastic stem cells, liver neoplasms, cell separation

CLC Number:

R394.2

Liu Yan, Zhou Yue-su, He Jing, Liu Peng. Liver cancer stem cell isolation and biological characteristics[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(28): 4549-4554.

Figures/Tables 5

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