COX-2 mRNA expression at different stages of osteoarthritis synoviocytes

doi:10.3969/j.issn.2095-4344.2014.07.004

Abstract

Abstract:

BACKGROUND: COX-2 gene actually exists in the joint fibroblast-like synoviocytes, it affects osteoarthritis occurrence and development. Understanding the differences of COX-2 gene expression levels at different stages of osteoarthritis synoviocytes has important theoretical significance for the occurrence and development of osteoarthritis, as well as the role of synoviocytes in this process.
OBJECTIVE: To analyze the difference of COX-2 mRNA at different stages of osteoarthritis fibroblast-like synoviocytes.
METHODS: Synovial membrane from 44 osteoarthritis patients and 12 normal cases were selected. Primary cells were cultured to passage 4 fibroblast-like synoviocytes for the use in the experiment. COX-2 mRNA expression in osteoarthritis fibroblast-like synoviocytes and normal fibroblast-like synoviocytes was detected using real-time fluorescence quantitative RT-PCR. The relative quantitative analysis was performed using 2^-ΔΔCt method.
RESULTS AND CONCLUSION: Expression of COX-2 mRNA in osteoarthritis fibroblast-like synoviocytes was significantly higher than that in normal fibroblast-like synoviocytes (P < 0.05). The expression levels reached a peak at early osteoarthritis group, with significant differences compared with middle and late osteoarthritis groups (P < 0.05). There was no significant difference between middle and later osteoarthritis groups (P > 0.05). COX-2 mRNA might be important biological marker for the inflammation in osteoarthritis, and mainly plays a role in early osteoarthritis stage.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

全文链接：

Key words: osteoarthritis, knee, synovial membrane, fibroblasts, reverse transcriptase polymerase chain reaction, COX-2

CLC Number:

R318

Zeng Ming-zhu, Duan Kan, Yuan Chang-shen, Mei Qi-jie, Qin Kai . COX-2 mRNA expression at different stages of osteoarthritis synoviocytes[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(7): 1003-1008.

Figures/Tables 5

References

[1] Rousseau JC, Delmas PD.Biological markers in osteoarthritis.Nat Clin Pract Rheumatol. 2007;3(6):346-356.

[2] 宋震坤,陈文照.环氧化酶-2与关节炎[J].中国骨伤,2003,16(5): 314-317.

[3] 方忠,李锋,熊伟,等.特异性COX-2抑制剂保护骨关节炎软骨的临床观察[J].实用骨科杂志,2006,12(1):29-31,32.

[4] 汤继文,刘新宇.骨关节病的诊治[J].山东医药,2000,40(15): 43-44.

[5] Kellgren JH, Lawrence JS.Radiological assessment of osteo-arthrosis.Ann Rheum Dis. 1957;16(4):494-502.

[6] 中华医学会骨科学分会.骨关节炎诊治指南(2007年版)[J].中华骨科杂志,2007,27(10):793-796.

[7] 孙贵才,徐轶尔,谢晶日,等.四种不同处理方法体外培养大鼠滑膜细胞[J].中国组织工程研究,2012,16(7):1193-1196.

[8] 蒋红,邢艳,杨明辉,等.不同来源人滑膜细胞体外培养的比较[J].四川解剖学杂志,2009,17(2):10-11.

[9] 樊佳佳,白俊杰,马冬梅,等.转红色荧光蛋白基因唐鱼外源基因拷贝数的测定[J].生物技术通讯,2011,22(1):61-65.

[10] Guilak F, Fermor B, Keefe FJ,et al.The role of biomechanics and inflammation in cartilage injury and repair.Clin Orthop Relat Res. 2004;(423):17-26.

[11] Bonnet CS, Walsh DA.Osteoarthritis, angiogenesis and inflammation.Rheumatology (Oxford). 2005;44(1):7-16.

[12] Livak KJ, Schmittgen TD.Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.Methods. 2001;25(4):402-408.

[13] Rousseau JC, Delmas PD.Biological markers in osteoarthritis.Nat Clin Pract Rheumatol. 2007;3(6):346-356.

[14] 杜国辉,贺艳丽,陈建英,等.骨关节炎早期诊断的生化指标[J].中国生化药物杂志,2009,30(3):209-211.

[15] Rüegg C, Mariotti A.Vascular integrins: pleiotropic adhesion and signaling molecules in vascular homeostasis and angiogenesis.Cell Mol Life Sci. 2003;60(6):1135-1157.

[16] 陈百成,张静.骨关节炎[M].北京:人民卫生出版社,2004:52-96.

[17] 张鹏,石关桐,郑昱新,等.补肾方、柔肝方与化痰方对膝骨关节炎滑膜细胞增殖及COMP表达的影响[J].中国中医骨伤科杂志, 2008,16(12):18-21.

[18] 朱锦宇,史曼,张伟,等.基因芯片法筛选膝骨关节炎滑膜相关差异表达基因[J].国际骨科学杂志,2010,31(6):384-386.

[19] 孙海兵,魏永长,涂宏蕾,等.COX-2、PKC-α和miR-101在胃癌中的表达及相关性[J].南方医科大学学,2013,33(4):559-562.

[20] Rask K, Zhu Y, Wang W,et al. Ovarian epithelial cancer: a role for PGE2-synthesis and signalling in malignant transformation and progression.Mol Cancer. 2006;5:62.

[21] 戴生明,韩星海,施冶青.两种选择性COX-2抑制剂治疗类风湿关节炎和骨关节炎的临床比较[J].中华风湿病学杂志,2000,4(6): 378.

[22] 舒强,李兴福,侯怀水,等.艾拉莫德对骨关节炎滑膜成纤维样细胞特性的影响[J].中华风湿病学杂志,2006,10(7):389-392.

[23] 王虹虹,曾红,申新国,等.核因子κBp65特异性小干涉核糖核酸抑制肿瘤坏死因子α诱导大鼠关节滑膜细胞中一氧化氮合酶2和环氧合酶2的表达[J].中国组织工程研究与临床康复,2007,11(27): 5295-5298.

[24] 姜林娣,王汉洲,郭津生,等. RNA干扰技术抑制类风湿关节炎成纤维细胞COX-2合成[J].复旦学报:医学版,2007,34(2): 302-312.