Effects of bone marrow mesenchymal stem cell conditioned medium on bioactivity of scar fibroblasts

doi:10.3969/j.issn.2095-4344.2014.07.005

Abstract

Abstract:

BACKGROUND: Mesenchymal stem cell transplantation promoted skin repair in trauma via various regulatory mechanisms and inhibited scar formation. At present, many scholars believed that bioactive factors secreted by mesenchymal stem cells played an important role.
OBJECTIVE: To investigate the effects of bone marrow mesenchymal stem cell conditioned medium on the proliferation and collagen synthesis of hypertrophic scar fibroblasts.
METHODS: Human bone marrow mesenchymal stem cells and hypertrophic scar fibroblasts were isolated and cultured, and bone marrow mesenchymal stem cell conditioned medium was prepared. Hypertrophic scar fibroblasts were cultured in vitro with 12, 24, and 48 hour-collected conditioned medium for 24 hours, which was compared with blank control group. The proliferation of cells was determined by CCK-8. Type I and type III collagen expression in hypertrophic scar fibroblasts was detected using real-time PCR.
RESULTS AND CONCLUSION: Compared with the blank control group, 24 and 48 hour-collected conditioned medium significantly inhibited the proliferation of hypertrophic scar fibroblasts (P < 0.01), and also suppressed collagen synthesis of hypertrophic scar fibroblasts (P < 0.01). Results suggested that bone marrow mesenchymal stem cell conditioned medium inhibited the proliferation and collagen synthesis of hypertrophic scar fibroblasts by secreting anti-fibrotic bioactive factors, which may provide new theoretical supports for cell therapy to reduce cutaneous scarring.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

全文链接：

Key words: bone marrow, mesenchymal stem cells, culture media, conditioned, cicatrix, fibroblasts, collagen type I, collagen type III, cell proliferation, hydroxyproline

CLC Number:

R318

Wu Yan, Zhang Chun-lei, Liu Yang, Li Hong-zhi, Yu Jing, Bao Hai-hua, Guo Ran, Yuan Xiao-huan. Effects of bone marrow mesenchymal stem cell conditioned medium on bioactivity of scar fibroblasts[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(7): 1009-1014.

Figures/Tables 1

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