In vitro fabrication of tissue-engineered osteochondral composite graft in a home-made double-chamber stirring bioreactor

doi:10.3969/j.issn.2095-4344.2015.16.006

Abstract

Abstract:

BACKGROUND: Articular cartilage injury is often complicated by subchondral bone damage to form osteochondral defects, and its treatment remains a pressing problem in orthopedics. Osteochondral composite grafts fabricated by tissue engineering technology provide a new way to repair osteochondral defects.
OBJECTIVE: To explore the feasibility of fabricating osteochondral composite grafts in an independently designed double-chamber stirring bioreactor.
METHODS: Goat bone marrow mesenchymal stem cells were seeded into β-tricalcium phosphate scaffolds. The cell/scaffold constructs were subjected to chondrogenic and osteogenic induction simultaneously in the double-chamber stirring bioreactor. According to the applied shear stress, they were divided into dynamic and static culture groups. The proliferation of bone marrow mesenchymal stem cells was detected by MTT test and scanning electron microscope examination. Osteogenesis and chondrogenesis related genes were detected by RT-PCR for the evaluation of chondrogenic and osteogenic differentiation.
RESULTS AND CONCLUSION: The bone marrow mesenchymal stem cells in both dynamic and static culture groups demonstrated satisfactory proliferation and differentiation. Better proliferation and differentiation ability were found in the dynamic culture group. Fabrication of osteochondral composite grafts in the independently.designed double-chamber stirring bioreactor is feasible, which is better under the mechanical stimulus environment than the static environment.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

全文链接：

Key words: Mesenchymal Stem Cells, Reverse Transcriptase Polymerase Chain Reaction, Cell Proliferation

CLC Number:

R318

Hu Yan-ling, Wang Ai-rong, Gong Hai-feng, Liu Hai-fei, Chen Feng, Qiao Guang-xi . In vitro fabrication of tissue-engineered osteochondral composite graft in a home-made double-chamber stirring bioreactor[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(16): 2489-2493.

Figures/Tables 3

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