ABCE1 gene silencing effect on biological characteristics of human cervical cancer cells XB1702

doi:10.3969/j.issn.2095-4344.2015.37.013

Abstract

Abstract:

BACKGROUND: With the development of genetic engineering and tumor molecular biology, gene therapy has acted as a new treatment mode for tumors.
OBJECTIVE: To investigate the impact of gene silencing ABCE1 on growth, proliferation and migration of human cervical cancer cells XB1702.
METHODS: siRNA sequences of ABCE1 were designed and synthesized to transfect XB1702 cells using LipofectamineTM 2000. Cells transfected with NC siRNA carrier served as controls, and untransfected cells acted as blank group. After RNA interference, the expression of ABCE1 mRNA and protein was detected by western blod assay; cell cycle was detected using flow cytometry; cell counting kit-8 test, scratch healing assay and cell invasion assay were used to evaluate the effects of ABCE1 silence gene on XB1702 cell proliferation, migration and invasion ability.
RESULTS AND CONCLUSION: ABCE1 mRNA and protein expression in the experimental group was significantly lower than that in the control group and blank group (P < 0.05). Growth rate in the experimental group was significantly slowed, cells were arrested in G0/G1 phase, and the number of S-phase cells was reduced. Compared with the control group and blank group, XB1702 cell proliferation was inhibited significantly in the experimental group, and cell migration and invasion was significantly decreased (P < 0.05). These results showed that specific interference with gene expression of ABCE1 can inhibit the migration of human cervical cancer cells XB1702, and restrain tumor cell proliferation; therefore, the siRNA sequence of ABCE1 may be an effective target for treatment of cervical cancer.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Uterine Cervical Neoplasms, RNA, Small Interfering, Gene Silencing, Cell Proliferation

CLC Number:

R318

Li Ping, Wang De-hua. ABCE1 gene silencing effect on biological characteristics of human cervical cancer cells XB1702[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(37): 5972-5977.

Figures/Tables 4

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