Effects of four decalcifying solutions on pathological structure of bone and bone marrow tissues

doi:10.3969/j.issn.2095-4344.2015.37.005

Abstract

Abstract:

BACKGROUND: As the bone and marrow tissue have very special structure, it is difficult to simultaneously display the bone with tough hard tissue and bone marrow tissues containing various immature hematopoietic cells in the conventional process of pathological section preparation.

OBJECTIVE: To choose the best decalcifying solution that cannot only completely remove the calcium in the bone tissue but also protect the structure of bone marrow tissues and cells from damage.

METHODS: Bone marrow tissues from the long bone of dogs were randomly divided into four groups. Under the same conditions, the bone marrow tissues were decalcified with 14% formaldehyde saline solution of nitric acid (group A), 14% nitric acid solution (group B), 20% A saline solution of hydrochloric acid formaldehyde (group C) and 20% A hydrochloric acid aqueous solution (group D). Decalcified time was recorded, followed by routine dehydration, section, hematoxylin-eosin staining and microscopic observation. Pathological section quality and hematoxylin-eosin staining were compared among the four groups.

RESULTS AND CONCLUSION: Group A had the best sections and hematoxylin-eosin staining, strongest decalcified ability, shortest decalcified time and minimum damage to the bone marrow. Group B had the worst results of section and hematoxylin-eosin staining, in which, the bone tissues were loose and became yellow and the bone marrow tissue were damaged greatly, and the decalcified effect was worse. Group C was worse than group A in decalcified ability, damage degree, section quality and hematoxylin-eosin staining results. Group D also had a better result of section and hematoxylin-eosin staining as well as exhibited uniform decalcification effect and less damage to the bone marrow, which was ranked between group B and group C. All the four kinds of decalcifying solutions have a good decalcification ability, but the section quality and hematoxylin-eosin staining results rank as follows: Group A > Group C > Group D > Group B. Taken together, 14% formaldehyde saline solution of nitric acid is ideal for the clinical preparation of pathological sections.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Decalcification Technique, Decalcification, Pathologic, Bone Marrow

CLC Number:

R318

Luo Can-qiao, Mo Mu-qiong, Nie Zhao-ming, Zhong Jue-min, Li Yu-bin. Effects of four decalcifying solutions on pathological structure of bone and bone marrow tissues [J]. Chinese Journal of Tissue Engineering Research, 2015, 19(37): 5928-5932.

Figures/Tables 1

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