Denervation effects on bone regeneration and expression of Runx2 during distraction osteogenesis

doi:10.3969/j.issn.2095-4344.2015.37.016

Abstract

Abstract:

BACKGROUND: During the healing of fractures, removal of sciatic nerve can result in insufficient mechanical rigidity of newborn woven bone. However, there are less reports concerning the denervation effects during distraction osteogenesis.

OBJECTIVE: To observe the effect of removal of the sciatic nerve on bone regeneration and the expression of Runt-related transcription factor 2 (Runx2) protein during distraction osteogenesis in a rabbit model.

METHODS: Twenty-four adult male New Zealand rabbits were selected and underwent left tibial osteodistraction to construct animal models of distraction osteogenesis. Before distraction, the animals were randomly divided into group R (resecting the left sciatic nerve) and group I (intact left sciatic nerve). Six weeks after completion of distraction, the animals were killed and the lengthened tibias were harvested for radiography, three-dimensional CT reconstruction, histological evaluation, connectivity density (Conn.D) evaluation.

RESULTS AND CONCLUSION: New regenerated bone was present and Runx2 protein was expressed in the distraction gaps of all animals at the end of the study, as revealed by radiography, three-dimensional CT reconstruction, and histological observation. However, less new bone formation and a lower degree of mineralization and expression of Runx2 protein were observed in group R compared with group I. The results suggest that the denervation appears to have an inhibitory effect on bone formation and the expression of Runx2 protein during distraction osteogenesis.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Tibia, Sciatic Nerve, Bone Regeneration, Biomechanics

CLC Number:

R318

Zheng Ke, Song Dong-hui, Feng Xing-mei, Zhu Song-song, Hu Jing2 Ye Bin. Denervation effects on bone regeneration and expression of Runx2 during distraction osteogenesis [J]. Chinese Journal of Tissue Engineering Research, 2015, 19(37): 5988-5992.

Figures/Tables 2

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