Modified primary culture of neonatal mouse myocardial cells

doi:10.3969/j.issn.2095-4344.2015.37.017

Abstract

Abstract:

BACKGROUND: A lot of work has been carried out on the development of the primary cultured rat myocardial cells at home and abroad. The primary culture technology of rat myocardial cells becomes more mature, but myocardial cells from neonatal mice are not easy to be obtained under the same experimental conditions. The mouse genome has more similarities with the human genome, which has a higher research value.
OBJECTIVE: To improve the primary culture method of neonatal mouse myocardial cells, and to obtain myocardial cells with high purity, vitality and original structure and function.
METHODS: The mouse cardiac tissues were treated using an enzyme digestion method to isolate isolated single myocardial cells: first, the cardiac tissues were digested using trypsin, and then collagenous fibers were treated with collagenase to isolate single myocardial cells. The concentration and action time of trypsin and type II collagenase were adjusted, and the pH values of reagents and temperature of each step were strictly controlled.
RESULTS AND CONCLUSION: At 24 hours after inoculation, the myocardial cells began to be adherent; at 48 hours, independent pulsation of myocardial cells could be observed; at 72 hours, myocardial cells were cross-linked; and at 96 hours, myocardial cells formed cell clusters and presented with consistent beating. The survival rate and purity of myocardial cells were both over 95%. This modified method could successfully culture myocardial cells with high purity and viablility from neonatal mice, and the structure and function of myocardial cells could be retained. Therefore, it is a feasible culture method.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Tissue Engineering, Myocytes, Cardiac, Mice

CLC Number:

R318

Meng Lin-lin, Huang Ying, Ma Yi-tong, Liu Fen, Chen Bang-dang, Chen Xiao-cui, Gai Min-tao. Modified primary culture of neonatal mouse myocardial cells[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(37): 5993-5997.

Figures/Tables 2

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