Chinese Journal of Tissue Engineering Research ›› 2021, Vol. 25 ›› Issue (4): 526-531.doi: 10.3969/j.issn.2095-4344.2364
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Liu Yang, Gong Yi, Fan Wei
Received:
2019-12-05
Revised:
2019-12-11
Accepted:
2020-02-22
Online:
2021-02-08
Published:
2020-11-21
Contact:
Fan Wei, MD, Chief physician, Second Department of Hepatobiliary Surgery, Guizhou Provincial People’s Hospital, Guiyang 550002, Guizhou Province, China
About author:
Liu Yang, Master, Chief physician, Second Department of Hepatobiliary Surgery, Guizhou Provincial People’s Hospital, Guiyang 550002, Guizhou Province, China
Supported by:
CLC Number:
Liu Yang, Gong Yi, Fan Wei. Anti-hepatoma activity of targeted Pluronic F127/formononetin nanocomposite system in vitro[J]. Chinese Journal of Tissue Engineering Research, 2021, 25(4): 526-531.
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2.1 PF-FA共轭傅里叶变换红外光谱的表征 PF、PF-NH2、叶酸和PF-FA的傅里叶变换红外光谱如图1所示。PF在 3 522.5 cm-1处出现典型的羟基峰,而PF-NH2在3 364, 1 521 cm-1处出现新的吸收峰,分别对应于胺基(N-H,宽)和羰基(C=O)峰[19]。在叶酸中,3 600-3 000 cm-1出现羟基(-OH)和N-H拉伸振动峰,羧基的C=O拉伸振动出现在 1 692 cm-1处,1 640 cm-1处出现峰值,这与-CO-NH基团的C=O键拉伸振动有关;1 605 cm-1处为N-H振动的弯曲模态,1 412 cm-1处为苯基骨架的-OH变形带,苯环的特征吸收峰出现在1 483 cm-1。在PF-FA中,3 399 cm-1(N-H)和1 521 cm-1 (C=O)处出现的吸收峰与PF-FA结构中的酰胺基(-CO-NH)有关。"
2.4 芒柄花黄素的包封率和体外释放 FN-PF和FN-PF-FA胶束的包封率分别为(84.12±2.15)%和(82.50±1.78)%,两种胶束具有很高的包封率,这可能是由于PF分子的非极性基团很大,形成了一个与FN相互作用的强疏水核,允许更多的包封。FN-PF和FN-PF-FA胶束的载药量分别为(21.33±2.27)%和(19.73±1.58)%。 两种胶束释放的芒柄花黄素如图3所示,在最初的 12 h(30%-40%)内观察到一次突释,随后进入缓释期,在相同pH值下,FN-PF-FA胶束释放芒柄花黄素的速率慢于FN-PF;同一胶束不同pH值下,pH=5.3时约80%的芒柄花黄素在5 d内释放,而同期只有约50%的芒柄花黄素在pH=7.4下释放(P < 0.01),这可能是由于在酸性pH值下芒柄花黄素的分散性较高[21]。"
2.5 体外抗癌实验结果 相对于空白对照组和对照组,芒柄花黄素组、FN-PF组、FN-PF-FA组的细胞数量减少,其中FN-PF-FA组细胞均呈现圆形,几乎无细胞贴壁生长,见图4。用GI50值来量化芒柄花黄素在PF-FA胶束制剂50%孵化细胞在设计时间段内的生长[22]。从图5A可以看出相对于芒柄花黄素,在相同浓度下FN-PF和FN-PF-FA胶束对HepG2细胞的抑制作用显著提高(P < 0.05);相同浓度下与FN-PF胶束对比,FN-PF-FA胶束对HepG2细胞抑制作用显著提高(P < 0.05)。 芒柄花黄素的GI50为(28.6±1.2) mg/L,FN-PF胶束的GI50大幅度降低至(17.4±0.78) mg/L,即降低了38.46%;FN-PF-FA胶束的GI50值为(9.5±0.4) mg/L,即与芒柄花黄素相比减少66.43%,与FN-PF胶束相比减少44.83%(P < 0.01),见图5B。"
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