Effect of cinnamaldehyde on osteoblasts in high glucose environment with the electrospinning membrane as a carrier

doi:10.3969/j.issn.2095-4344.3229

Abstract

Abstract: BACKGROUND: Previous studies have found that cinnamaldehyde can promote the biological properties of osteoblasts in high glucose environment.
OBJECTIVE: To observe the effect of cinnamaldehyde on proliferation and differentiation of osteoblasts under high glucose with electrospinning membrane as carrier.
METHODS: The polycaprolactone membranes were prepared by electrospinning. MC3T3-E1 cells in logarithmic growth phase were divided into four groups: blank control group was added with high glucose medium. Polycaprolactone group was added with polycaprolactone membrane scaffold and high glucose medium. Drug group was added with high glucose medium containing cinnamaldehyde. Combined group was added with polycaprolactone membrane and high glucose medium containing cinnamaldehyde. CCK-8 method was used to detect cell proliferation and alkaline phosphatase kit was used to detect alkaline phosphatase activity at 1, 4 and 7 days of culture. RT-qPCR was used to detect the expression of osteocalcin mRNA at 7 days.
RESULTS AND CONCLUSION: (1) CCK-8 assay showed that the cell proliferation of polycaprolactone group, drug group and combined group was faster than that of blank control group at each time point (P < 0.05). Cell proliferation of combined group was faster than that of polycaprolactone group and drug group at 4 and 7 days (P < 0.05), and cell proliferation of drug group was faster than that of polycaprolactone group at 4 and 7 days (P < 0.05). (2) The alkaline phosphatase activity of polycaprolactone group, drug group, and combined group was higher than that of blank control group at 4 and 7 days (P < 0.05). The alkaline phosphatase activity of combined group was higher than that of polycaprolactone group and drug group at 4 and 7 days (P < 0.05). The alkaline phosphatase activity of drug group was higher than that of polycaprolactone group at 7 days (P < 0.05). (3) The expression of osteocalcin mRNA in the four groups from high to low was the combined group, drug group, polycaprolactone group, and blank control group; there were significant differences between the two groups (P < 0.05). (4) Results suggest that polycaprolactone electrospinning membrane and cinnamaldehyde can promote the proliferation and differentiation of osteoblasts in high glucose environment.

Key words: bone, materials, polycaprolactone, electrospinning membrane, carrier, cinnamaldehyde, MC3T3-E1 cells, proliferation, differentiation, high glucose environment, dual effects

CLC Number:

Liu Keke, Duan Xin, Ma Xiangrui, Zhang Yuntao. Effect of cinnamaldehyde on osteoblasts in high glucose environment with the electrospinning membrane as a carrier[J]. Chinese Journal of Tissue Engineering Research, 2021, 25(22): 3500-3504.

Figures/Tables 4

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