iTRAQ technology is important to screen serum markers of differentially expressed proteins in knee osteoarthritis

doi:10.3969/j.issn.2095-4344.2015.37.010

Abstract

Abstract:

BACKGROUND: There is lack of effective diagnostic methods for early knee osteoarthritis. Proteomics refers to the large-scale experimental analysis of proteomes from the overall level of intracellular protein compositioon, expression and modification, based on which, we can understand protein-protein interaction and relationship, thereby revealing protein functions and cell activity patterns.
OBJECTIVE: To apply the isobaric tags for relative and absolute quantitation (iTRAQ) to identify proteins differentially in serums of osteoarthritis Kellgren-Lawrence classification at each stage, and to find out potential molecular markers at each stage of osteoarthritis.
METHODS: Sixty patients with knee osteoarthritis were enrolled, and according to Kellgren-Lawrence (K-L) grading, they were subdivided into K-L 0, II and IV groups. There were 10 males and 10 females randomly selected in each group. Proteins differentially regulated in serums were identified by the stable isotopes 116, 117, 118 of the iTRAQ labeled, reversed-phase column separation, mass spectrometry and Swissport database search, after the serums were subjected to high abundance proteins depletion. Finally, we analyzed the proteins identified by using bioinformatics software.
RESULTS AND CONCLUSION: Totally 169 proteins were identified through iTRAQ peptides experiments of different sample tags, Q-star mass spectrum identification and MASCOT library search. 153 proteins were identified as K-L 0 to K-L IV, 153 proteins were identified as K-L 0 to K-L II, and 145 proteins were identified as K-L II to K-L IV. iTRAQ technology may help to identify novel serum markers for early diagnosis of knee osteoarthritis, indicating iTRAQ technology for proteomics serum biomarkers of osteoarthritis study has good application prospect.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Osteoarthritis, Proteomics, Adiponectin

CLC Number:

R318

Liang Hai-bo, Su Wei, Luo Shi-xing, Dong Gui-fu, Long Guang-hua, Qin Yu-jie. iTRAQ technology is important to screen serum markers of differentially expressed proteins in knee osteoarthritis[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(37): 5955-5960.

Figures/Tables 3

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