Chinese Journal of Tissue Engineering Research ›› 2021, Vol. 25 ›› Issue (25): 3981-3987.doi: 10.12307/2021.008

Previous Articles     Next Articles

Comparison on the curative effect of human placenta-derived mesenchymal stem cells and induced islet-like cells in gestational diabetes mellitus rats

Gao Shan1, Huang Dongjing1, Hong Haiman1, Jia Jingqiao2, Meng Fei1   

  1. 1Department of Obstetrics, Affiliated Central Hospital of Shenyang Medical College, Shenyang 110024, Liaoning Province, China; 2WellCare Stem Cell Biotech (Liaoning) Co., Ltd., Benxi 110024, Liaoning Province, China
  • Received:2020-06-17 Revised:2020-06-24 Accepted:2020-08-04 Online:2021-09-08 Published:2021-03-24
  • Contact: Meng Fei, Master’s supervisor, Professor, Department of Obstetrics, Affiliated Central Hospital of Shenyang Medical College, Shenyang 110024, Liaoning Province, China
  • About author:Gao Shan, Master, Associate chief physician, Department of Obstetrics, Affiliated Central Hospital of Shenyang Medical College, Shenyang 110024, Liaoning Province, China
  • Supported by:
    the Science and Technology Fund of Shenyang Medical College, No. 20182040 (to GS); the Scientific Research Project of Shenyang Health and Family Planning Commission (to GS)

Abstract: BACKGROUND: Gestational diabetes can cause serious damage to maternal and fetal health. Exploring safe and effective methods on gestational diabetes prevention and treatment has become one of the important research directions of obstetrics and gynecology.
OBJECTIVE: To compare the therapeutic effects of human placenta-derived mesenchymal stem cells and islet-like cells on gestational diabetes mellitus rats, so as to provide theoretical basis for clinical research on the treatment of gestational diabetes mellitus.
METHODS:  Human placenta-derived mesenchymal stem cells were extracted from human placental tissue under aseptic conditions, then the identified stem cells were induced into islet-like cells. Flow cytometry was used to detecte the expressions of insulin and nestin protein in differentiated cells and dithizone staining was used to identify the expression of zinc ions in islet-like cell clusters. Female Sprague-Dawley rats were divided into five groups (n=10 per group): Normal pregnant rats group, gestational diabetes mellitus rats without intervention group, gestational diabetes mellitus rats saline intervention group, gestational diabetes mellitus rats human placenta-derived mesenchymal stem cells intervention group and gestational diabetes mellitus rats islet-like cells intervention group. Gestational diabetes mellitus model was established by feeding high-glucose and high-fat diet before pregnancy and intraperitoneal injection of streptozotocin after pregnancy. After successful modeling, 1 mL normal saline suspension containing 6×106 human placenta-derived mesenchymal stem cells or islet-like cells was injected via tail vein. The fasting blood glucose and body weight of rats in each group were measured at 6, 12, and 18 days of pregnancy, and the serum adiponectin level of pregnant rats was detected at 18 days of pregnancy.   
RESULTS AND CONCLUSION: (1) During the differentiation of human placenta-derived mesenchymal stem cells into islet-like cells, the insulin level increased and the nestin level increased then decreased gradually. The islet-like cell clusters formed at 28 days. (2) Both human placenta-derived mesenchymal stem cells and islet-like cells treatment were effective on reducing blood glucose and promoting adiponectin levels as well as body weight. At 18 days, compared with human placenta-derived mesenchymal stem cells, islet-like cells reduced blood glucose and increased adiponectin levels and body weight more significantly. (3) The results indicate that both human placenta-derived mesenchymal stem cells and islet-like cells treatments were effective on gestational diabetes mellitus rats. Islet-like cells induced by human placenta-derived mesenchymal stem cells treatment were more effective than that of the uninduced human placenta-derived mesenchymal stem cells.

Key words: stem cells, human placenta-derived mesenchymal stem cells, islet-like cells, induced differentiation, gestational diabetes mellitus, blood glucose, adiponectin

CLC Number: