Dynamical effect of fractures combined with brain injury on the bone healing and bone metabolism

doi:10.3969/j.issn.2095-4344.2015.37.002

Abstract

Abstract:

BACKGROUND: Peri-fracture nerve injury can inhibit osteoclast activity and promote early fracture healing.

OBJECTIVE: To investigate dynamically the effects of traumatic brain injury on the bone mineral density, microstructure, biomechanics property and bone metabolism in rat models of fractures.

METHODS: Sixty-three male rats were randomly divided into three groups: sham group, simple fracture group and fracture combined with brain injury group. After 3, 6, and 3 months, the animals were sacrificed in batches under anesthesia, and then, the bones and serum specimens were used to detect the bone mineral density, microstructure, biomechanics property, serum cross-linked N-telopeptide of collagen type I and osteocalcin levels.

RESULTS AND CONCLUSION: Compared with the simple fracture group, the fracture combined brain injury group had significantly increased bone mineral density of the proximal tibia, bone volume fraction of the cancellous bone, trabecular thickness, cross-sectional area of tibial cortical bone and total area of the bone marrow, ultimate load and stress of the tibia, serum cross-linked N-telopeptide of collagen type I and osteocalcin levels at 3 and 6 weeks after modeling (P < 0.05), but no differences in the above-mentioned indexes were found among the three groups at 3 months after modeling. These findings indicate that traumatic brain injury can increase the bone mineral density at the fracture site, improve bone microstructure and enhance biomechanical properties, thereby promoting bone healing and bone metabolism at the fracture site.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Tissue Engineering, Fractures, Bone, Biomechanics, Nerve Growth Factor

CLC Number:

R318

Zhou Qing, Liu Jin-lian, Liu Chao-qun, Zhou Yao-dong, Chen Hao. Dynamical effect of fractures combined with brain injury on the bone healing and bone metabolism[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(37): 5911-5915.

Figures/Tables 3

References

[1] 曾凡瑞,谭文甫.骨折合并脑损伤时骨折愈合加速的研究[J].社区医学杂志,2012,10(13):13-15.

[2] Marmarou A. A new model of diffuse brain injury in rats. Pathophysiology and biomechanics. J Neurosurg. 1994;80(2): 301-313.

[3] Roberts JM. Multiple injuries: management of the patient with a fractured femur and a head injury. J Bone Joint Surg. 1968; 42(3):425.

[4] Boes M, Kain M, Kakar S, et al. Osteogenic effects of traumatic brain injury on experimental fracture-healing. J Bone Joint Surg Am. 2006;88(4):738-743.

[5] Zwerina J, Redlich K, Polzer K, et al. TNF- induced structural Joint Damage is mediated by IL-1. Proc Natl Acad Sci USA. 2007;104(28):11742-11747.

[6] Bidner SM, Rubins IM, Desjardins JV, et al. Evidence for a humoral mechanism for enhanced osteogenesis after head injury. J Bone Joint Surg Am. 1990;72(8):1144-1149.

[7] Perkin SR, Skirving AP. Callus formation and the rate of healing of femoral fractures in patients with head injuries. J Bone Joint Surg. 1987;69(4):521.

[8] 沈红雷,周悦.脑损伤合并骨折愈合加速的原因分析[J].中外医学研究,2011,9(11):121-123.

[9] Dalle Carbonare L, Valenti MT, Bertoldo F, et al. Bone microarchitecture evaluated by histomorphometry. Micron. 2005;36(7/8):99-105.

[10] 刘丽君,罗湘杭,廖二元,等.男性骨特异性碱性磷酸酶、骨钙素、Ⅰ型胶原氨基末端肽与骨密度的关系[J].中国骨质疏松杂志, 2007,13(11):783-787.

[11] 赵重熙,马军,何宁,等.局部应用神经生长因子对周围神经损伤后骨折早期愈合的影响[J].中国组织工程研究,2015,19(15):2320- 2324.

[12] 杨森,王海龙,盛伟斌,等.骨折合并脊髓损伤患者血清中转化生长因子β1变化:有利于促进骨折愈合[J].中国组织工程研究, 2015, 19(2):165-169.

[13] 赵金平.大鼠脑损伤合并骨折时低氧诱导因子-1对骨折愈合的影响[J].临床和实验医学杂志,2013,12(8):572-576.

[14] 方玲娜,戴如春,盛志峰,等.去卵巢大鼠胫骨和椎骨松质骨微结构的显微CT分析[J].中国组织疏松杂志,2011,17(9):757-760.

[15] 李展春,刘祖德,戴力扬,等.MicroPET/CT评价去卵巢大鼠骨代谢变化的实验研究[J].中国骨质疏松杂志,2014,20(8):875-879.

[16] 刘巍,粱志刚,张先锋.转化生长因子β在骨质疏松骨折愈合过程中表达的实验研究[J].中国药物与临床,2013,13(12): 1562-1564.

[17] 王海龙,盛伟斌,徐韬,等.大鼠股骨骨折合并脊髓损伤模型的构建[J].中国组织工程研究,2014,18(18):2818-2823.

[18] 朱生根,苏利强,徐明.神经生长因子与被动运动对失神经大鼠骨代谢指标的影响[J].中国骨质疏松杂志,2014,20(6):606-609.

[19] 王立明,张伟,陈坤,等.神经生长因子及其受体在下颌骨骨折愈合中的表达及意义[J].实用口腔医学杂志,2011,27(4):460-464

[20] 张冉,孙宏志.神经生长因子在合并颅脑损伤的股骨干骨折修复中的意义[J].中华神经医学杂志,2010,9(12):1265-1267.

[21] 贾考田.神经生长因子对84例骨折患者愈合情况的临床观察[J].北方药学,2013,(5):90.

[22] 李东海,周安宁.周围神经损伤后局部应用神经生长因子对骨折早期愈合的影响[J].中国实用神经疾病杂志,2014,17(22):68-69.

[23] 张贵春,张永.神经生长因子对合并神经损伤胫骨骨折早期愈合的影响[J].中国组织工程研究与临床康复,2009,13(11):2057- 2061.

[24] 曹玲,杨光.增龄与骨骼肌肌细胞的退变[J].中国老年学杂志, 2014,(10):2893-2896.

[25] 李西华,赵蕾,吴燕,等.神经源性一氧化氮合酶对临床疑似Becker型肌营养不良的诊断意义[J].中国当代儿科杂志,2011,13(4): 288-291.

[26] 粟谋,徐威,蒋林彬,等.影响神经生长因子表达因素的研究进展[J].重庆医学,2014,56(6):735-737.