Microtubule-associated protein-2: regulating neuronal development, structural stability, projection formation and synaptic plasticity

doi:10.3969/j.issn.2095-4344.2015.37.020

Abstract

Abstract:

BACKGROUND: Microtubule-associated protein-2 is a kind of important regulatory factor in regulating tubulin assembly. As one of the main members of microtubule-associated proteins, microtubule-associated protein-2 plays an important role in the repairing and development of the nervous system function. It has been found that microtubule-associated protein-2 can promote the repair and rebuilding of injured nerves.

OBJECTIVE: To summarize the relationship between microtubule-associated protein-2 and nerve injury as well as the mechanism of action.

METHODS: The PubMed database and CNKI database were retrieved by the fist author for the articles related to microtubule-associated protein-2 published from January 1976 to January 2015. The key words were “microtubule-associated protein-2 (MAP-2), nerve injury, progress” in English and Chinese, respectively. In the same field, articles published recently or in authorized journals were preferred. Repetitive or old articles were excluded, and finally 82 articles were included in result analysis.

RESULTS AND CONCLUSION: Microtubule-associated protein-2 is involved in nerve repair, and plays a promoting role in neuronal morphology and plasticity. To increase the concentration of microtubule-associated protein-2 contributes to the recovery of neurologic function in the early stage after nerve injury.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Microtubule-Associated Proteins, Neurons, Neoplasms

CLC Number:

R318

Yao Zhong-kai, Wu Zuo-pei, Sun Gui-xin. Microtubule-associated protein-2: regulating neuronal development, structural stability, projection formation and synaptic plasticity[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(37): 6010-6016.

Figures/Tables 1

References

[1] Ainsztein AM, Purich DL.Stimulation of tubulin polymerization by MAP-2.Control by protein kinase C-mediated phosphorylation at specific sites in the microtubule-binding region. J Biol Chem.1994.269(45):28465-28471.

[2] Panda D, Goode BL, Feinstein SC,et al. Kinetic stabilization of microtubule dynamics at steady state by tau and microtubule- binding domains of tau. Biochemistry. 1995;34(35):17-27.

[3] Sloboda RD, Dentler VT, Rosenbaum JL. Microtubule- associated proteins and the stimulation of tubulin assembly in vitro. Biochemistry.1976;15(20):4497-4505.

[4] Shafrt-Zagardo B, Kalcheva N. Making sense of the multiple MAP-2 transcripts and their role in the neuron.Mol Neurobiol. 1998;16(2):149-162.

[5] Neve RL, Hams P, Kosik KS, et al.Identification of cDNA clones for the human microtubule-associated protein tau and chromosomal localization of the genes for tau and microtubule- associated protein 2. Brain Res.1986 387:271-280.

[6] Shafit-Zagardo B, Rockwood J, Davies P, et al. Novel microtubule-associated protein - 2 isoform is expressed early in human oligodendrocyte maturation. Glia.2000;29 (3 ): 233-245.

[7] Huber G，Matus A.Differences in the cellular distributions of two microtubule-associated proteins ,MAP1 and MAP-2,in rat brain J Neurosci.1984;4(1):151-160.

[8] Turker RP,Binder LI,Viereck C,et al.The sequential appearance of low and high-molecular weight farms of MAP-2 in the developing cerebellum. J Neurosci. 1988;8(12):A503-512.

[9] Kindler S,Schulz B,Goedert M,et al.Molecular structure of microtubule-associated protein 2b and 2c from rat brain) Biol Chem. 1990;265(32):19679-19684.

[10] Lewis SA,Wang DH,Cowan NJ.Microtubule-associated protein 2MAP2 shares a microtubule binding motif with tau protein. Science. 1988;242, 936-939.

[11] Papandrikopoulou A, Doll T, Turker RP,et al.Embryonic MAP-2 lacks the crossing-linking sidearm sequences and dendritic targeting singnal of adult MAP-2.Nature.1989;340 :639-645.

[12] Doll T,Meichsner M,Riederer BM,et al. An isoform of microtubule- associated protein 2 (MAP-2) containing four repeats of the tubulin-binding motif. J Cell Sci. 1993; 106(Pt 2):633-639.

[13] Ferhat L, Bernard A, Ribasd PL,et al. regional and developmental expression of rat MAP-2d, a MAP-2 splice variant ncoding four microtubule-binding domains.Neurochem Int. 1994;25(4):327-338.

[14] Fenralli J, Doll T, Matus A. Sequence analysis of MAP-2 function in living cells.J Cell Sci. 1994; 107(Pt 11):3115-3125.

[15] Obar RA, Dingus J, Bayley H,et al. The RU subunit of CAMP-dependent protein kinase binds to a common amino-terminal domain in microtubule-associated proteins 2A,2B, and 2C Neuron. 1989;3(5):639-645.

[16] Rubino HM,Dammerman M,Shafit-Zagardo B,et al. Localization and characterization of the binding site for the regulatory subunit of type Ⅱ cAMP-dependent protein kinase on MAP-2. Neuron.1989;3(5):631-638.

[17] Kalcheva N,Weidenheim KM,Kress Y,et al. Expression of microtubule-associated protein-2a and other novel microtubule-associated protein-2 transcripts in human fetal spinal cord. J Neuruchem.1997;68(1)383-391.

[18] Kindler S, Garner CC.Four repeat MAP-2 isoforms in* and rat brain. Brain Res Mol Brain Res. 1994;26(1-2):218-224.

[19] Garner CC, Matus A.Different forms of microtubule- associated protein 2 are encoded by separate mRNA transcripts. J Cell Biol.1988; 106(3):779-83

[20] Viereck C, Tucker RP, Matus A.The adult rat olfactory system expresses microtubule-associated proteins found in the developing brain J Neurosci.1989;9(10):354-357.

[21] Caceres A, Payne MR, Binder LI,et al.Immunocytochemical localization of actin and microtubule-associatedactin protein MAP2 in dendritic spines.Proc Natl Acad Sci USA. 1983;80(6): 1738-1742.

[22] Langnaese K, Seidenbecher C, Wex H, et al. Protein components of a rat brain synaptic junctional protein preparation. Brain Res Mol Brain Res.1996;42(1):118-122.

[23] Papasozomenos SC, Binder LI, Bender PK,et al. Microtubule-associated protein 2 within axons of spinal motor neurons: associations with microtubules and neurofilaments in normal and beta,beta'-iminodipropionitrile-treated axons.J Cell Biol.1985; 100(1):74-85.

[24] Meichsner M, Doll T, Reddy D,et al. The low molecular weight form of microtubule-associated protein 2 is transported into both axons and dendrites. Neuroscience. 1993;54(4): 873-880.

[25] Sharma N, Kress Y, Shaftt ZB. Antisense MAP-2 oligonucleotides induce changes in microtubule assembly and neuritic elongation in pre-existing neuritis of rat cortical neurons. Cell Motil Cytoskeleton.1994;27(3):234-247.

[26] Boucher M, Belanger D, Beaulieu C,et al. Tau-mediated process outgrowth is differentially altered by the expression of MAP2b and MAP2c in Sf9 cells. Cell Motil Cytoskeleton. 1999;42(4):257-73.

[27] Huang YA, Kao JW, Tseng DT, et al. Microtubule-Associated Type II Protein Kinase A Is Important for Neurite Elongation. PloS one.2013;8(8): e73890.

[28] Woolf NJ, Zinnerman MD, Johnson GV.Hippocampal microtubule-associated protein-2 alterations with contextual memory.Brain Res.1999;821(1):241-249.

[29] Serrano L, Avila J, Maccioni RB, Controlled proteolysis of tubulin by subtilisin: localization of the site for MAP2 interaction. Biochemistry.1984;23:4675-4681.

[30] Cross D, Dominguez J, Maccioni RB,et al. MAP-1 and MAP-2 binding sites at the C-terminus of beta-tubuhn. Biochemistry 1991;15:133-168.

[31] Saoudi Y, Paintrand I, Multigner L,et al. Stabilization and bundling of subtilisin-treated microtubules induced by microtubule associated proteins. J Cell Sci. 1995;108 Pt 1):357-367.

[32] Al-Bassam J, Ozer RS, Safer D,et al. MAP-2 and tau bind longitudinally along the outer ridges of microtubule protofilaments. J Cell Biol.2002;157(7):1187-1196.

[33] Ichihara K, Kitazawa H, Iguchi Y,et al. Visualization of the stop of microtubule depolymerization that occurs at the high-density region of microtubule-associated protein 2 (MAP-2).J Mol Biol. 2001;312(1):107-118.

[34] Vandecandelaere A, Pedrotti B, Utton MA,et al. Differences in the regulation of microtubule dynamics by microtubule- associated proteins MATIB and MAP-2. Cell Motil Cytoskeleton.1996;35(2):134-146.

[35] Pryer NK, Walker RA, Skeen VP,et al.Brain microtubule- associated proteins modulate microtubule dynamic instability in vitro. Real-time observations using video microscopy. J Cell Sci.1992; 103 ( Pt 4):965-76.

[36] Wang U, Colella R, Roisen FJ. Ganglioside GM 1 alters neuronal morphology by modulating the association of MAP-2 with microtubules and actin filaments.Brain Res Dev Brain Res. 1998;105(2):227-239.

[37] Correas I, Padilla R, Avila J. The tubulin-binding sequence of brain microtubule-associated proteins, tau and MAP-2, is also involved in actin binding.Biochem 1990;269(1): 61-64.

[38] Kwei SL, Clement A, Faissner A,et al. Differential interactions of MAP-2, tau and MAP5 during axogenesis in culture. Neuroreport.1998; 9(6):1035-1040.

[39] Heimann R, Shelanski ML, Liem RK.Microtubule-associated proteins bind specifically to the 70-kDa neurofilament protein. J Biol Chem.1985;260(22):12160-12166.

[40] Teng J, Takei Y, Harada A, et al. Synergistic effects of MAP-2 and MAPIB knockout in neuronal migration, dendritic outgrowth, and microtubule organization. J Cell Biol. 2001; 155(1):65-76.

[41] Chakravarthy B, Rashid A, Brown L, et al. Association of Gap-43 (neuromodulin) with microtubule-associated protein MAP-2 in neuronal cells. Biochemical and biophysical research communications.2008;371(4): 679-683.

[42] Ferhat L, Represa A, Bernard A, et al. MAP2d promotes bundling and stabilization of both microtubules and microfilaments. J Cell Sci.1996;109:1095-1103.

[43] Ludin B, Ashbridge K, Funfschilling U,et al.Functional analysis of the MAP-2 repeat domain. J Cell Sci. 1996;109 (Pt 1): 91-99.

[44] Mandelkow E,Hoenger A.Structures of kinesin and kinesin- microtubule-microtubule interactions.Curr Opin Cell Biol. 1999;11(1):34-44.

[45] Hagiwara H, Yorifuji H, Sato YR,et al. Competition between motor molecules (kinesin and cytoplasmic dynein) and fibrous microtubule-associated proteins in binding to microtubules. J Biol Chem. 1994;269(5):3581-3589.

[46] Lopez LA, Sheetz MP.Steric inhibition of cytoplasmic dynein and kinesin motility by MAP-2. Cell Motil Cytoskeleton. 1993; 24(1):1-16.

[47] Ulitzur N, Humbert M, Pfeffer SR. Mapmodulin: a possible modulator of the interaction of microtubule-associated proteins with microtubules. Proc Natl Acad Sci USA. 1997; 94(10):5084-5089.

[48] Tsuyama S,Terayama Y,Matsuyama S.Numerous phosphates of microtubule-associated protein 2 in living rat brain .J Biol Chem.1987;262(22):10886-10892.

[49] Diez-Guerra FJ, Avila JJ. MAP-2 phosphorylation parallels dendrite arborization in hippocampal neurones in culture. Neuroreport. 1993;4(4):419-422.

[50] Diaz-Nido J, Montoro RJ, Lopez-Bameo J,et al. High external potassium induces an increase in the phosphorylation of the cytoskeletal protein MAP-2 in rat hippocampal slices. Eur J Neurosci.1993;5(7):818-824.

[51] Matsuno A, Takekoshi S, Sanno N, et al. Modulation of protein kinases and microtubule-associated proteins and changes in ultrastructure in，female rat pituitary cells: effects of estrogen and bromocriptine.J Histochem Cytochem.1997;45(6):805-813.

[52] Brugg B, Matus A.Phosphorylation determines the binding of microtubule- associated protein 2 (MAP-2) to microtubules in living cells.J Cell Biol.1991;114: 735-743

[53] Springer J, Azbill RD, Kennedy S,et al.Rapid calpain I activation and cytoskeletal protein degradation following traumatic spinal cord injury: attenuation with riiuzole pretreatment. J Neurochem. 1997;69(4):1592-1600.

[54] Maddodi N, Bhat KM, Devi S, et al. Oncogenic BRAFV600E induces expression of neuronal differentiation marker MAP2 in melanoma cells by promoter demethylation and down-regulation of transcription repressor HES1. J Biol Chem. 2010;285(1): 242-254.

[55] Liu Y, Sturgis CD, Grzybicki DM,et al. Microtubule-associated protein-2: a new sensitive and specific marker for pulmonary carcinoid tumor and small cell carcinoma.Mod Pathol. 2001; 14(9):880-885.

[56] Surridge CD,Burns RG. The difference in the binding of phosphatidylinositol distinguishes MAP-2 from MAP-2C and Tau.Biochemistry.1994;33(26):8051-8057.

[57] Scaife RM, Wilson L, Purich DL. Microtubule protein ADP-ribosylation in vitro leads to assembly inhibition and rapid depolymerization.Biochemistry. 1992;31(1):310-316.

[58] Fang D, Hallman J, Sangha N,et al. Expression of microtubule-associated protein 2 in benign and malignant melanocytes: implications for differentiation and progression of cutaneous melanoma. Am J Pathol.2001; 158(6):2107-2115.

[59] Song Z, He C D, Sun C, et al.Increased expression of MAP2 inhibits melanoma cell proliferation, invasion and tumor growth in vitro and in vivo[J]. Experimental dermatology. 2010;19(11): 958-964.

[60] Dinsmore JH, Solomon Y. Inhibition of MAP 2 expression affects both morphological and cell division phenotypes of neuronal differentiation.Cell.1991;64(4) :817-826.

[61] Cheu J, Kauai Y, Cowau NJ, et al.Projection domains of MAP2 and tau determine spacings between microtubules in dendrites and axons.Nature.1992;360(6405):674-677.

[62] NunezJ. Immature and mature variants of MAP2 and tan proreins and neuronal plasticity. Trends Neurosci. 1988;11 (11) : 477-479.

[63] Folkerts MM，Bermau RF, Muizelaar JY, et al. Disruption of MAP-2 immunostaining in rat hippocampus after traumatic brain injury. J Neurotrauma. 1998;15(5):349-363.

[64] Holgado A, Ferreira A. Synapse formation proceeds independently of dendritic elongation in cultured hippocampal neurons. J Neurobiol.2000;43(2): 121-131.

[65] Sanchez Martin C, Ledesma D, Dotti CG ,et al. Microtubule - associated protein - 2 located in growth regions of rat hippocampal neurons is highly phosphorylated at its proline - rich region. Neuroscience.2000;101(4):885-893.

[66] Waterman-Storer CM, Salmon E. Positivefeedback interactions between microtubule and actin dynamics during cell motility. Curr Opin Cell Biol.1999; 11(1):61-67.

[67] Beltramino CA. Timed changes of synaptic zinc, synaptophysin and MAP< sub> 2</sub> in medial extended amygdala of epileptic animals are suggestive of reactive neuroplasticity. Brain research.2010;1328: 130-138.

[68] Tang L,Lu Y, Zheng W, et al. Overexpression of MAP-2 via Formation of Microtubules Plays an Important Role in the Sprouting of Mossy Fibers in Epileptic Rats. J Mol Neurosci. 2014; 53(1):103-108.

[69] Guo Y, Sanchez C, Udin SB. MAP2 phosphorylation and visual plasticity in Xenopus. Brain Res, 2001;905 (1-2) :134-141.

[70] Song ZM , Undie AS, Koh PO ,et al. D1 dopamine receptor regulation of microtubule-associated protein-2 phosphorylation in developing cerebral cortical neurons. J Neurosci. 2002;22(14): 6092-6105.

[71] Terabayashi T, Itoh TJ, Yamaguchi H,et al.Polarity-regulating kinase partitioning-defective 1/microtubule affinity-regulating kinase 2 negatively regulates development of dendrites on hippocampal neurons. J Neurosci..2007; 27(48): 13098-13107.

[72] Hayashi K, Kawai - Hirai R, Ishikawa K, et al. Reversal of neuronal polarity characterized by conversion of dendrites into axons in neonatal rat cortical neurons in vitro. Neuroscience. 2002; 110(1):7-17.

[73] Tatebayashi Y, Lee MH, Li L ,et al. The dentate gyrus neurogenesis: a therapeutic target for Alzheimer’s disease. Acta Neuropathol Ber.2003;105 (3) :225-232.

[74] Yamanouchi H, Jay V , Otsubo H , et al. Early forms of microtubule - associated protein are strongly expressed in cortical dysplasia. Acta Neuropathol.1998;95 (5 ):466-470.

[75] Choudhary S,Joshi K,Gill KD.Possible role of enhanced microtubule phosphorylation in dichlorvos induced delayed neurotoxicity in rat.Brain Res.2001;897(1-2 ):60-70.

[76] Hatakeyama T, Matsmmto M，Brengman JM，et al. Immurmhistochemical investigation of ischemic and postischemic damage after bilateral carotid occlusion in gerbils.Stroke.1988;19(12):1526-1534.

[77] Pettigrew LC, Holtz ML, Cradbock SP, et al. Microtubular proteolysis in focal cerebral ischemia.J Cereb Blood Flow Metab.1996;16(6):1189-1202.

[78] Kitagawa K, Matsumoto M, Niinobe M, et al. Microtubule- associated protein -2 as a sensitive marker for cerebral ischemic damage-immunohistochemical investigation of dendritic damage. Neuroscience.1989; 31 (2):401-411.

[79] Kaech S, Parmar H, Roelandse M, et al. Cytoskeletal microdiffer2entiation: a mechanism for organizing morphological plasticity in dendrites. Proc Natl Acad Sci USA.2001;98(13): 7086-7092.

[80] Monshausen M, Putz U, Rehbein M, et al.Two rat brain staufen isoforms differentially bind RNA J Neurochem. 2001; 76( 1 ):155-165.

[81] Burtelow MA, Longacre TA. Utility of microtubule associated protein-2 (MAP-2) immunohistochemistry for identification of ganglion cells in paraffin-embedded rectal suction biopsies. Am J Surg Pathol. 2009;33(7):1025-1030.

[82] Iwata M,Muneoka KT,Shirayama Y,et al.A study of a dendritic marker, microtubule-associated protein 2 (MAP-2), in rats neonatally treated neurosteroids, pregnenolone and dehydroepiandrosterone (DHEA). Neuroscience letters.2005; 386(3):145-149.