MicroRNA-10b regulates aldehyde dehydrogenase 1 mRNA and protein expression in breast cancer MCF-7 cell line

doi:10.3969/j.issn.2095-4344.1622

Abstract

Abstract:

BACKGROUND: MicroR-10b can regulate the characteristics of breast cancer stem cells, and acetaldehyde dehydrogenase 1 (ALDH1) is one of the most important markers of breast cancer stem cells. The interaction between them in breast cancer cells needs further explorations.
OBJECTIVE: To investigate whether over-expression of microRNA-10b affects ALDH1 mRNA and protein levels in human breast cancer MCF-7 cells.
METHODS: hsa-miR-10b mimics or its negative control was transfected into breast cancer MCF-7 cell line. At 48 hours after transfection, the mRNA and protein expression of ALDH1 in the cells was detected using real-time RT-PCR and western blot assays, respectively.
RESULTS AND CONCLUSION: Over-expression of microR-10b was found in the MCF-7 cell line transfected with hsa-miR-10b mimics, which was significantly higher than that in the control group (P=0.003 47). Both of ALDH1 mRNA and protein levels were up-regulated in the MCF-7 cell line overexpressing microR-10b, as compared with the control group (P=0.009 54 and P=0.003 11, respectively). To conclude, over-expression of microR-10b induces the ALDH1 mRNA and protein expression in the breast cancer MCF-7 cell line, providing new evidence that microR-10b regulates the invasion and metastasis of breast cancer cells.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Breast Neoplasms, Neoplastic Stem Cells, MicroRNAs, Aldehyde Dehydrogenase, Tissue Engineering

CLC Number:

Han Mingli, Lü Pengwei, Qian Xueke, Yang Xue, Yang Yunqing, Gu Yuanting. MicroRNA-10b regulates aldehyde dehydrogenase 1 mRNA and protein expression in breast cancer MCF-7 cell line[J]. Chinese Journal of Tissue Engineering Research, 2019, 23(9): 1349-1353.

Figures/Tables 3

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