中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (19): 3487-3491.doi: 10.3969/j.issn.1673-8225.2012.19.014

• 脐带脐血干细胞 umbilical cord blood stem cells • 上一篇    下一篇

在纤维蛋白凝块上诱导脐血间充质干细胞向膀胱尿路上皮细胞的分化★◆

广茜茜1，韩玉环2，畅继武3   

  1. 1天津医科大学，天津市泌尿外科研究所国家重点实验室，天津市  300070；2天津医科大学第二医院，天津市 300211；3天津市泌尿外科研究所，天津市  300211
  • 收稿日期:2012-01-18 修回日期:2012-02-26 出版日期:2012-05-06 发布日期:2012-05-06
  • 通讯作者: 畅继武，教授，天津市泌尿外科研究所，天津市 300211 changjiwu2004@ yahoo.com.cn
  • 作者简介:广茜茜★，女，1986年生，山东省济宁市人，汉族，2012年天津医科大学毕业，硕士，主要从事膀胱尿路上皮细胞的培养、干细胞的培养与诱导分化研究。Guangqianqian1986@163.com

Induced differentiation of umbilical cord blood mesenchymal stem cells into urothelial cells on fibrin clots

Guang Qian-qian1, Han Yu-huan2, Chang Ji-wu3   

  1. 1Tianjin Institute of Urinary Surgery, the State Key Laboratory of China, Tianjin Medical University, Tianjin  300070, China; 2Second Hospital of Tianjin Medical Unversity, Tianjin  300211, China; 3Tianjin Institute of Urinary Surgery, Tianjin  300211, China
  • Received:2012-01-18 Revised:2012-02-26 Online:2012-05-06 Published:2012-05-06
  • Contact: Chang Ji-wu, Professor, Tianjin Institute of Urinary Surgery, Tianjin 300211, China changjiwu2004@ yahoo.com.cn
  • About author:Guang Qian-qian★, Master, Tianjin Institute of Urinary Surgery, the State Key Laboratory of China, Tianjin Medical University, Tianjin 300070, China Guangqianqian1986@163.com

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354

被引次数

1

摘要：

背景：在纤维蛋白凝块上诱导脐血间充质干细胞分化为膀胱尿路上皮细胞，尚无明确方案。
目的：探讨脐血来源间充质干细胞在纤维蛋白凝块上分化为膀胱尿路上皮细胞的可能性。
方法：实验组将脐血间充质干细胞接种于人工制备的脐血来源的纤维蛋白凝块上与胎儿膀胱尿路上皮细胞共培养，对照组将脐血间充质干细胞接种于人工制备的脐血来源的纤维蛋白凝胶上仅用DMEM/F1培养基培养。
结果与结论：实验组的脐血间充质干细胞逐渐伸展呈多角形，细胞扁平变大，透射电子显微镜下具有膀胱尿路上皮细胞的超微结构，抗广谱角蛋白(AE1/AE3)免疫组织化学染色阳性。对照组的脐血间充质干细胞呈长梭形，抗广谱角蛋白(CK AE1/AE3)免疫组织化学染色阴性。提示在纤维蛋白凝块上诱导脐血间充质干细胞有分化为膀胱尿路上皮细胞的能力。
 

关键词: 纤维蛋白凝块, 脐血间充质干细胞, 尿路上皮细胞, 分化, 共培养

Abstract:

BACKGROUND: Whether umbilical cord blood mesenchymal stem cells (UCB-MSCs) can be induced to differentiate into urothelial cells on fibrin clots remains poorly understood.
OBJECTIVE: To investigate the possibility of UCB-MSCs differentiating into urothelial cells on fibrin clots.
METHODS: In the experimental group, the UCB-MSCs on umbilical cord blood fibrin clot were co-cultured with fetal urothelial cells. In the control group, the UCB-MSCs on umbilical cord blood fibrin clot were cultured in culture medium of DMEM/F12.
RESULTS AND CONCLUSION: In the experimental group, the UCB-MSCs gradually became larger and were polygonal. The cells turned over their ultrastructure to urothelial cells under transmission electron microscope and were positive for immunhistochemistry staining of anti-cytokeratins (AE1/AE3). In the control group, the UCB-MSCs morphology presented spindle-like arrangement and were negative for immunohistochemistry staining of anti-cytokeratins (AE1/AE3). The UCB-MSCs on fibrin clot have the potential to differentiate into urothelial cells.

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