中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (11): 2069-2073.doi: 10.3969/j.issn.1673-8225.2011.11.042

• 骨组织构建 bone tissue construction • 上一篇    下一篇

护骨素基因T245G多态性与老年人骨密度关系的部位及性别差异

陈陵霞，苗懿德，刘  杰，魏雅楠，郏  蓉，宝  辉，褚  琳   

  1. 北京大学人民医院老年科，北京市  100044
  • 收稿日期:2010-10-12 修回日期:2011-01-06 出版日期:2011-03-12 发布日期:2011-03-12
  • 作者简介:陈陵霞☆，女，1972年生，重庆市人，汉族，2001年北京大学医学部毕业，博士，副主任医师，副教授，主要从事骨质疏松症及2型糖尿病并发症方面的研究。
  • 基金资助:

    北京大学人民医院研究发展基金(RDI2008-02)。

Association between T245G polymorphisms in the osteoprotegerin gene and bone mineral density in elderly individuals

Chen Ling-xia, Miao Yi-de, Liu Jie, Wei Ya-nan, Jia Rong, Bao Hui, Chu Lin   

  1. Department of Geriatrics, Peking University People’s Hospital, Beijing 100044, China
  • Received:2010-10-12 Revised:2011-01-06 Online:2011-03-12 Published:2011-03-12
  • About author:Chen Ling-xia☆, Doctor, Associate chief physician, Associate professor, Department of Geriatrics, Peking University People’s Hospital, Beijing 100044, China chenlingxia@ medmail.com.cn
  • Supported by:

    Research Development Foundation of Peking University People’s Hospital, No. RDI2008-02*

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摘要：

背景：作为骨折发生的重要临床预测因子，骨密度在一定程度上由遗传因素决定。护骨素基因是骨质疏松症发病中的重要候选基因。
目的：探讨护骨素基因T245G多态性与骨密度的相关性。
方法：选取2008-09/2010-04在北京大学人民医院进行常规查体的老年人281名，其中男182名，女99名。应用PCR-RFLP结合DNA测序检测护骨素基因T245G多态性，使用双能X射线骨密度测量仪测定受试者腰椎、髋部标准位置及前臂的骨密度。同时收集受试者的生化指标及临床观察项目。应用ANOVA方法分析护骨素基因T245G多态性与各检测指标的关系。
结果与结论：在老年男性及绝经后女性中，T245G基因T，G等位基因频率分布差异无显著性意义(P > 0.05)。在老年男性中，GG和TG基因型具有较高的腰椎骨密度，而TT基因型的腰椎骨密度较低(P < 0.05)，Ward’s三角区及前臂骨密度在各基因型间差异无显著性意义(P > 0.05)。在绝经后女性中，T245G多态性与骨密度无关，说明护骨素基因与老年男性腰椎骨密度有关。

关键词: 护骨素, 基因, 多态性, 骨密度, 骨质疏松症

Abstract:

BACKGROUND: As a primary clinical predictor of fracture risk, bone mineral density (BMD) is partly genetically determined. Osteoprotegerin (OPG) is one important candidate gene in the pathogenesis of osteoporosis.
OBJECTIVE: To investigate the association between T245G polymorphisms in the OPG gene and BMD.
METHODS: A total of 281 elderly men and postmenopausal women, 182 males and 99 females, who received routine examinations at Peking University People’s Hospital between September 2008 and April 2010 were included in this study. T245G polymorphisms in the OPG gene was detected by polymerase chain reaction-restriction fragment length polymorphism together with DNA sequencing. The BMD of the lumbar spine, Ward’s triangle, and forearrm was determined by dual energy X-ray absorptiometry. Clinical variables and biochemical measurements were collected simultaneously. The association between T245G polymorphisms and each detection index was analyzed using analysis of variance.
RESULTS AND CONCLUSION: The distribution of T245G genotype (alleles T, G) had no difference in elderly men or postmenopausal women (P > 0.05). The GG genotype and TG genotype had higher lumbar spine BMD and TT genotype had lower lumbar spine BMD (P < 0.05). There was no difference in BMD of the Ward’s triangle or forearm among different genotypes (P > 0.05). Association between T245G polymorphism and BMD was not found in postmenopausal women. These findings indicate that OPG gene is related to lumbar spine BMD in elderly men.

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