中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (18): 3231-3234.doi: 10.3969/j.issn.1673-8225.2010.18.001

• 肝移植 liver transplantation •    下一篇

胸腺诱导对大鼠肝移植的免疫影响

张  岩，陈曦海，纪艳超，翟  哲，吴  波   

  1. 哈尔滨医科大学附属第四医院普外科，黑龙江省哈尔滨市  150001
  • 出版日期:2010-04-30 发布日期:2010-04-30
  • 通讯作者: 吴 波，硕士，教授，硕士生导师，主要从事胃肠外科的研究。 xinxin9129@126.com
  • 基金资助:

    黑龙江省攻关项目(GC09C409-1)，黑龙江省教育厅资助项目(11541147)。

Immune effect of thymus induction on rat liver transplantation

Zhang Yan, Chen Xi-hai, Ji Yan-chao, Zhai Zhe, Wu Bo   

  1. Department of General Surgery, Fourth Hospital Affiliated to Harbin Medical University, Harbin   150001, Heilongjiang Province, China
  • Online:2010-04-30 Published:2010-04-30
  • Contact: Wu Bo, Master, Professor, Master’s supervisor, Department of General Surgery, Fourth Hospital Affiliated to Harbin Medical University, Harbin 150001, Heilongjiang Province, China xinxin9129@126.com
  • Supported by:

    the Key Project of Heilongjiang Province, No. GC09C409-1*; the Foundation Project of Heilongjiang Department of Education, No. 11541147*

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摘要：

背景：异体肝脏移植由主要组织相容性抗原可诱发免疫排斥反应，免疫抑制剂会对机体产生不良反应，目前通过移植前对供受体进行预处理等策略，可以诱导受体产生免疫耐受，在移植中具有广阔的应用前景。
目的：用大鼠胸腺诱导的方法，对大鼠肝移植进行处理，从胸腺诱导方面，研究胸腺诱导与移植免疫排斥反应的关系。
方法：供体为清洁级雄性SD大鼠40只，受体为清洁级雄性Wistar大鼠30只和清洁级雄性SD大鼠10只，分为同种基因移植组、同种异基因移植组、环孢素组、胸腺修饰诱导组，各10对。采用改良Kamada二袖套法并加以改进建立稳定的大鼠原位肝移植模型，造模后，环孢素组每天腹腔注射环孢素(50 mg/kg)，持续5 d，胸腺修饰诱导组胸腺左右两叶各注射50 µL 主要组织相容性抗原，持续5 d，其他组不给予任何干预措施，记录各组大鼠生存时间并分别于移植后3，7，14，21，28 d进行病理学观察和混合淋巴细胞培养。
结果与结论：经胸腺修饰诱导的肝移植大鼠生存时间则显著延长(> 60 d)，移植肝内组织损伤程度显著减轻，肝脏局部免疫排斥反应减少，淋巴细胞减少，肝移植效果与同种基因移植大鼠接近，且优于环孢素干预大鼠(P < 0.05)。提示胸腺诱导可减轻大鼠肝移植后产生的免疫排斥反应。

关键词: 胸腺诱导, 免疫排斥, 肝移植, 环孢素, 基因

Abstract:

BACKGROUND: During xenogenic liver transplantation, major histocompatibility antigen can induce immunological rejection, and immunosuppressant can cause adverse effect on organism. Recently, treatment prior to transplantation induces immune tolerance, which is perspective for organ transplantation.
OBJECTIVE: To investigate the correlation between thymus induction and immunological rejection during liver transplantation.

METHODS: A total of 40 male SD rats of clean grade were selected as donors. Moreover, 30 male Wistar rats of clean grade and 10 male SD rats of clean grade were selected as recipients. The donor rats were divided into allogeneic gene transplantation, allotransplantation, cyclosporine, and thymus induction groups, with 10 rats in each group. The modified Kamada and improved two-cuff technique was used to establish a stable rat orthotopic liver transplantation model. The cyclosporine group was given cyclosporine (50 mg/kg) for 5 successive days. Thymus induction group was injected with major histocompatibility antigens (50 µL) for 5 successive days. Other groups were not given any interventions. Survival time of rats was recorded in each group. Pathological observation and mixed lymphocyte cultured were performed at days 3, 7, 14, 21, and 28 after transplantation.

RESULTS AND CONCLUSION: Survival time was longer in the thymus induced group compared with other groups (> 60 days), damaged level was mild, local immunological rejection was reduced, and lymphocytes were decreased. The effect after liver transplantation was similar to allogeneic gene transplantation but superior to cyclosporine intervention (P < 0.05). This suggested that thymus induction relieved immunological rejection following liver transplantation.

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