中国组织工程研究 ›› 2018, Vol. 22 ›› Issue (18): 2789-2794.doi: 10.3969/j.issn.2095-4344.0786

• 组织工程骨及软骨材料 tissue-engineered bone and cartilage materials •    下一篇

超临界二氧化碳法构建淫羊藿苷-聚乳酸/羟基磷灰石仿生型骨修复材料

张育敏1,王建茹2,韩 波1,牛晓军1   

  1. 1山西中医药大学,山西省太原市 030024;2山西医科大学,山西省太原市 030006
  • 收稿日期:2018-01-11 出版日期:2018-06-28 发布日期:2018-06-28
  • 通讯作者: 张育敏,副教授,山西中医药大学,山西省太原市 030024
  • 作者简介:张育敏,男,1977 年生,山西省阳城县人,汉族,2007年南方医科大学毕业,博士,副教授,主要从事中医药在再生医学中的应用研究。
  • 基金资助:

    山西省自然科学基金项目(201601D011126);山西中医药大学博士启动基金项目(2014bk05);山西省中医药科研课题(2016ZYYC02)

Preparation of icariin-poly(lactic acid)/hydroxyapatite composite bone-graft substitutes by supercritical carbon dioxide method

Zhang Yu-min1, Wang Jian-ru2, Han Bo1, Niu Xiao-jun1   

  1. 1Shanxi University of Chinese Medcine, Taiyuan 030024, Shanxi Province, China; 2Shanxi Medical University, Taiyuan 030006, Shanxi Province, China
  • Received:2018-01-11 Online:2018-06-28 Published:2018-06-28
  • Contact: Zhang Yu-min, Shanxi University of Chinese Medcine, Taiyuan 030024, Shanxi Province, China
  • About author:Zhang Yu-min, M.D., Associate professor, Shanxi University of Chinese Medcine, Taiyuan 030024, Shanxi Province, China
  • Supported by:

    the Natural Science Foundation of Shanxi Province of China, No. 201601D011126; Scientific Research Fund for the Doctoral Young Scholars, Shanxi University of Chinese Medicine, No. 2014bk05; Shanxi Scientific Research Fund of Traditional Chinese Medicine, No. 2016ZYYC02

摘要:

文章快速阅读:

 

文题释义:
超临界二氧化碳合成法:一种聚合物制备的新技术,被广泛应用于物质分离、提纯、分析化学、材料制造、药物缓释、化学反应等各个方面,具有环境友好、反应过程无需引入高温和有机溶剂、无需药物溶解等优点,特别适合含药物、生长因子等生物活性材料的制备。
淫羊藿苷:来源于传统中药淫羊藿的植物黄酮——淫羊藿苷,已有研究证实淫羊藿苷对干细胞的增殖和成骨分化具有促进作用,可作为一种良好的骨诱导活性因子替代物。此外,淫羊藿苷来源广泛、提取工艺相对简单、性质稳定、易于储存、可耐受消毒灭菌,这些特性均有利于其在组织工程支架材料中的应用。
 
摘要
背景:前期实验采用超临界二氧化碳法制备了具有良好理化性能和生物相容性的聚乳酸/羟基磷灰石材料,但该材料仅具有骨传导活性,缺乏骨诱导活性。
目的:构建具有骨传导和骨诱导活性的淫羊藿苷-聚乳酸/羟基磷灰石仿生型骨修复材料。
方法:通过超临界二氧化碳法制备每克材料中含淫羊藿苷10-4,10-5,10-6 mmol的淫羊藿苷-聚乳酸/羟基磷灰石骨修复材料,分别记为IC-PLA/HA(1000)、IC-PLA/HA(100)和IC-PLA/HA(10),以聚乳酸/羟基磷灰石骨修复材料为对照,通过生物力学性能、孔隙率、缓释特性、扫描电镜检测,筛选出淫羊藿苷的最佳复合比例。

结果与结论:①IC-PLA/HA(1000)组、IC-PLA/HA(100)组和IC-PLA/HA(10)组的压缩强度与弹性模量与对照组比较均无差异;②各组材料孔隙率均大于75%,IC-PLA/HA(1000)组、IC-PLA/HA(100)组和IC-PLA/HA(10)组的孔隙率与对照组比较均无差异;③IC-PLA/HA(1000)组、IC-PLA/HA(100)组和IC-PLA/HA(10)组材料淫羊藿苷释放在前3 d较快,以后逐渐下降,且下降速率减慢,但7 d后淫羊藿苷释放处于一个缓慢下降的平台期,其中IC-PLA/HA(100)组各时间点淫羊藿苷释放量接近前期实验证实的淫羊藿苷有效浓度10-7 mmol/L;④扫描电镜显示IC-PLA/HA(100)组材料中,羟基磷灰石与聚乳酸混合均匀,可见微球,淫羊藿苷在材料中不易识别,材料孔径在50-150 μm之间;⑤结果表明,构建的淫羊藿苷-聚乳酸/羟基磷灰石仿生型骨修复材料具有良好的力学性能及缓释性能。

ORCID: 0000-0002-0702-3067(张育敏) 

关键词: 淫羊藿苷, 聚乳酸, 羟基磷灰石, 骨修复材料, 超临界二氧化碳, 山西省自然科学基金, 生物材料

Abstract:

BACKGROUND: Our previous findings have shown that poly(lactic acid)/hydroxyapatite (PLA/HA) composite biomaterials prepared by the supercritical carbon dioxide method have good physiochemical properties and biocompatibility. However, the composite materials only have osteoconductivity but no osteoinductivity.

OBJECTIVE: To prepare icariin-PLA/HA (IC-PLA/HA) composite biomaterials with good osteoconduction and osteoinduction.
METHODS: The supercritical carbon dioxide method was used to prepare IC-PLA/HA composite biomaterials containing 10-4, 10-5 10-6 mmol/g IC, named as IC-PLA/HA(1 000), IC-PLA/HA(100) and IC-PLA/HA(10). PLA/HA composite material served as controls. Biomechanical properties, porosity, sustained release characteristics were detected, and scanning electron microscope observation was performed, in order to screen out the optimal IC content in the composite materials.
RESULTS AND CONCLUSION: (1) Compressive strength and elastic modulus of IC-PLA/HA(1 000), IC-PLA/HA(100) and IC-PLA/HA(10) showed no difference from those of PLA/HA. (2) The porosity of all the composite materials was over 75%, and there was still no difference among groups. (3) The IC release from IC-PLA/HA was faster within the first 3 days, and then reduced gradually. However, after 7 days, the IC release plateaued, and the IC release amount from the IC-PLA/HA(100) was close to 10-7 mol/L that had been confirmed to be an effective and safe concentration in the previous experiments. (4) Under the scanning electron microscope, HA and PLA were mixed homogeneously and IC was difficult to be identified. The pore size of the IC-PLA/HA(100) ranged from 50 μm to 150 μm. Overall, the IC-PLA/HA composite biomaterials have good mechanical and sustained-release properties. 

Key words: Drugs, Chinese Herbal, Hydroxyapatites, Biomimetic Materials, Tissue Engineering

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