中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (50): 9353-9355.doi: 10.3969/j.issn.1673-8225.2011.50.011

• 组织构建细胞学实验 cytology experiments in tissue construction • 上一篇    下一篇

持续静水压对细胞增殖的影响

郭紫芬，冯  勇，李  凯，廖端芳   

  1. 南华大学药物药理研究所，湖南省衡阳市 421001
  • 收稿日期:2011-06-22 修回日期:2011-07-01 出版日期:2011-12-10 发布日期:2011-12-10
  • 通讯作者: 廖端芳，博士，教授，博士研究生导师，南华大学药物药理研究所，湖南省衡阳市 421001 dfliao66@yahoo.com.cn
  • 作者简介:郭紫芬☆，女，1976年生，湖南省沅江市人，汉族，博士，副教授，主要从事心血管药理学方面的研究。 guozifen@yahoo.com.cn
  • 基金资助:

    国家863专题项目(2008AA02Z436)、国家自然科学基金(81102516)和湖南省研究生创新项目(CX2009B191)。

Effect of continuous static hydrostatic pressure on cell proliferation

Guo Zi-fen, Feng Yong, Li Kai, Liao Duan-fang   

  1. Institute of Pharmacy and Pharmacology, University of South China, Hengyang 421001, Hunan Province, China
  • Received:2011-06-22 Revised:2011-07-01 Online:2011-12-10 Published:2011-12-10
  • Contact: Liao Duan-fang, Doctor, Professor, Doctoral supervisor, Institute of Pharmacy and Pharmacology, University of South China, Hengyang 421001, Hunan Province, China dfliao66@yahoo.com.cn
  • About author:Guo Zi-Fen☆, Doctor, Associate professor, Institute of Pharmacy and Pharmacology, University of South China, Hengyang 421001, Hunan Province, China guozifen@yahoocom.cn
  • Supported by:

    the National High Technology Research and Development Program of China (863 Program), No. 2008AA02Z436*; the National Natural Science Foundation of China, No. 81102516*; the Graduate Innovation Project of Hunan Province, No. CX2009B191*

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614

被引次数

1

摘要：

背景：血管平滑肌细胞在静水压替代血清培养的条件下能良好生长。
目的：探讨无血清水平状态下，持续性静水压对细胞增殖的影响。
方法：采用自行研制的压力可调细胞培养箱在0，12，16，20，24和28 kPa条件下培养血管平滑肌细胞A10与血管内皮细胞HUVEC-12。
结果与结论：A10细胞在16 kPa压力的条件下增殖最明显，活性最强；HUVEC-12细胞在20 kPa压力的条件下增殖最明显，活性最强。说明一定范围内的静水压可促进体外培养的A10与HUVEC-12细胞增殖、使其活性增加。

关键词: 高静水压, 无血清细胞培养, 血管内皮细胞, 血管平滑肌细胞, 增殖

Abstract:

BACKGROUND: Vascular smooth muscle cells grow well in culture condition using static hydrostatic pressure instead of serum.
OBJECTIVE: To investigate the effect of continuous static hydrostatic pressure on the proliferation of vascular smooth muscle cells.
METHODS: Vascular smooth muscle cell A10 and vascular endothelial cell HUVEC-12 were cultured in a self-developed pressure adjustable incubator, under different pressures of 0, 12, 16, 20, 24 and 28 kPa respectively.
RESULTS AND CONCLUSION: The most obvious proliferation of A10 cell was detected under the pressure of 16 kPa, with greatest cell activity. And the most obvious proliferation of HUVEC-12 cell was under 20 kPa pressure, with greatest cell activity. It confirms that limited hydrostatic pressure can promote the proliferation of A10 and HUVEC-12 and increase the cell activity.

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