中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (50): 9413-.doi: 10.3969/j.issn.1673-8225.2010.50.026

• 组织构建学术探讨 • 上一篇    下一篇

构建酒精性股骨头坏死动物模型的理论依据及造模方法

王大伟，史宝明，张爽   

  1. 广西中医学院附属瑞康医院，广西壮族自治区南宁市 530001
  • 出版日期:2010-12-10 发布日期:2010-12-10
  • 通讯作者: 史宝明，广西中医学院在读硕士，广西中医学院附属瑞康医院，广西壮族自治区南宁市 530001 26826713@qq.com
  • 作者简介:王大伟，男，1956年生，上海市人，汉族，教授，主任医师，主要从事骨与关节退变与缺血性疾病的防治研究。 26826713@qq.com
  • 基金资助:

    广西卫生厅重点科研课题(重200853)，项目名称：三七总皂苷对兔股骨头缺血性坏死成骨细胞护骨素基因表达的影响。

Theoretical basis and preparation of animal models of alcoholic avascular necrosis of the femoral head  
 

Wang Da-wei, Shi Bao-ming, Zhang Shuang   

  1. Affiliated Ruikang Hospital of Guangxi College of Traditional Chinese Medicine, Nanning  530001, Guangxi Zhuang Autonomous Region, China
  • Online:2010-12-10 Published:2010-12-10
  • Contact: Shi Bao-ming, Studying for master’s degree, Affiliated Ruikang Hospital of Guangxi College of Traditional Chinese Medicine, Nanning 530001, Guangxi Zhuang Autonomous Region, China 26826713@qq.com
  • About author:Wang Da-wei, Professor, Chief physician, Affiliated Ruikang Hospital of Guangxi College of Traditional Chinese Medicine, Nanning 530001, Guangxi Zhuang Autonomous Region, China
  • Supported by:

     the Key Subjects of Health Department of Guangxi Zhuang Autonomous Region, No.200853*

PDF

642

被引次数

28

摘要：

背景：酒精性股骨头坏死晚期股骨头易塌陷、致残率高，且目前缺乏有效的防治方法，故备受国内外学者的关注，因此，建立酒精性股骨头坏死动物模型，是进一步探讨其发病机制及临床防治的重要研究方向。
目的：总结国内外对酒精性股骨头坏死动物模型建立的研究进展。
方法：应用计算机分别在PubMed数据库和中国期刊全文数据库(CNKI)中检索1990-01/2010-01有关酒精性股骨头坏死的临床和基础实验研究的文章，检索词分别为“alcohol intoxication; avascular necrosis; animal model; research progress”和“酒精中毒；股骨头坏死；动物模型；研究进展”，纳入所述内容与酒精性股骨头坏死的动物模型相关的文献，排除发表时间较早或重复性研究。
结果与结论：收集到151篇相关文献，排除121篇不符合标准的文献，共纳入30篇符合标准的文献。经分析得出以下结论：通过对鼠、兔等动物过度饮酒或股骨头局部酒精介入，可导致股骨头髓内脂肪细胞增多、增大，小血管数量减少或阻塞，造成微循环障碍导致其缺血、缺氧而最终发生坏死，其发生机制接近于人类股骨头缺血性坏死的早期病理改变。结合国内外对酒精性股骨头坏死动物模型的研究现状，采用灌胃法，给予中国白兔烈性中国白酒(含体积分数55%乙醇)8 mL/(kg•d)4~8周处理，此种造模方法更适合目前实验研究。

关键词: 酒精中毒, 股骨头坏死, 灌胃法, 动物模型, 研究进展

Abstract:

BACKGROUND: Alcoholic avascular necrosis of femoral head results in femoral head collapse at late stage, which has high disability and lack of effective prevention and treating methods. Thus, it has aroused increasing attention all over the world. To establish animal alcoholic avascular necrosis of femoral head model is a direction of studying pathogenesis and clinical prevention of this disease.
OBJECTIVE: To summarize of research progress of alcoholic avascular necrosis of the femoral head.
METHODS: PubMed and China journal full-text databases were searched by computer for articles regarding alcoholic avascular necrosis of the femoral head published from January 1990 to January 2010 with key words of “alcohol intoxication, avascular necrosis, animal model and research progress” both in English and Chinese. The reproducibility or obsolete study was excluded. 
RESULTS AND CONCLUSION: A total of 151 literatures were collected, and 30 articles were included in the analysis. Based on rats and rabbits animals such as binge drinking alcohol, femoral or local intervention can lead to increased femoral intramedullary fat cells and blood vessels, small quantity or obstruction of small vessels, result in microcirculation disturbance and ischemia or anoxia, finally, become necrosis. This mechanism was close to early pathological changes of human avascular necrosis. Combining domestic alcoholic avascular necrosis of animal model, using gastric lavage method, given by China rabbit spirited Chinese liquor (including ethanol 55%) 8 mL/(kg•d), for 4-8 weeks. The method is more suitable for the current domestic experiments.

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