中国组织工程研究

中国组织工程研究 ›› 2020, Vol. 24 ›› Issue (26): 4213-4217.doi: 10.3969/j.issn.2095-4344.2768

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

三七皂苷R1保护四氯化碳诱导肝纤维化模型大鼠的作用

吴  朕1，马  微2，臧成昊1，刘矿嫔2，刘  伟2，刘  洁2，梁  宇2，李春艳2，陈志明1，茹  金1，樊楚明1，杨金伟1，郭建辉1，李力燕2   

  1. 1昆明理工大学附属医院，云南省第一人民医院，云南省昆明市   650032；2昆明医科大学，云南省昆明市   650500

  • 收稿日期:2019-12-27 修回日期:2020-01-04 接受日期:2020-02-19 出版日期:2020-09-18 发布日期:2020-09-02
  • 通讯作者: 李力燕，博士，教授，昆明医科大学，云南省昆明市 650500 郭建辉，教授，昆明理工大学附属医院，云南省第一人民医院，云南省昆明市 650032 杨金伟，硕士，主治医师，昆明理工大学附属医院，云南省第一人民医院，云南省昆明市 650032
  • 作者简介:吴朕，男，1992年生，安徽省泗县人，汉族，昆明理工大学在读硕士，主要从事肝脏疾病相关领域、肝癌细胞转移机制方向的研究。
  • 基金资助:
    国家自然科学基金项目(31560295)；云南省科技厅昆明医科大学联合专项重点项目[2018FE001(-163)]；云南省科技厅昆明医科大学联合专项重点项目[2019FE001(-179)]；云南省心血管疾病影像研究中心(2017NS254，2018NS0270)；云南省创新团队(2019HC022)；云南省万人计划(YNWR-MY-2018-015)；昆明医科大学重大科技成果培育项目(CGPY201802)

Protective effect of notoginsenoside R1 on carbon tetrachloride-induced liver fibrosis in rats

Wu Zhen1, Ma Wei2, Zang Chenghao1, Liu Kuangpin2, Liu Wei2, Liu Jie2, Liang Yu2, Li Chunyan2, Chen Zhiming1, Ru Jin1, Fan Chuming1, Yang Jinwei1, Guo Jianhui1, Li Liyan2   

  1. 1The Affiliated Hospital of Kunming University of Science and Technology, the First People’s Hospital of Yunnan Province, Kunming 650032, Yunnan Province, China; 2Kunming Medical University, Kunming 650500, Yunnan Province, China

  • Received:2019-12-27 Revised:2020-01-04 Accepted:2020-02-19 Online:2020-09-18 Published:2020-09-02
  • Contact: Li Liyan, MD, Professor, Kunming Medical University, Kunming 650500, Yunnan Province, China Guo Jianhui, Professor, the Affiliated Hospital of Kunming University of Science and Technology, the First People’s Hospital of Yunnan Province, Kunming 650032, Yunnan Province, China Yang Jinwei, Master, Attending physician, the Affiliated Hospital of Kunming University of Science and Technology, the First People’s Hospital of Yunnan Province, Kunming 650032, Yunnan Province, China
  • About author:Wu Zhen, Master candidate, the Affiliated Hospital of Kunming University of Science and Technology, the First People’s Hospital of Yunnan Province, Kunming 650032, Yunnan Province, China
  • Supported by:
     the National Natural Science Foundation of China, No. 31560295; Yunnan Provincial Science and Technology Department & Kunming Medical University Joint Special Key Project, No. 2018FE001 (-163)  and 2019FE001 (-179); Yunnan Cardiovascular Disease Imaging Research Center, No. 2017NS254 and 2018NS0270 ; Yunnan Provincial Innovation Team, No. 2019HC022; Yunnan Provincial Ten Thousand Talent Project, No. (YNWR-MY-2018-015); the Major Scientific and Technological Achievement Cultivation Project of Kunming Medical University, No. CGPY201802

PDF

103

摘要：

文题释义：
三七皂苷R1：分子式为C47H80O18，属原人参三醇型，是三七中最具代表性的特征化合物，有抗凝血、抑制缺血再灌注损伤和清除氧自由基等作用。三七皂苷R1对中枢神经系统、免疫系统、心血管系统具有较好的预防和缓解作用，还具有抗癌、抗炎、抗辐射、改善微循环等作用，具有良好的市场前景。 
肝纤维化：是一个病理生理过程，是指由多种致病因子所致肝内结缔组织异常增生，胶原合成增多在胞外基质形成纤维状束带。肝脏损伤修复愈合的过程中都有肝纤维化的过程，损伤因素长期不能去除，长期持续纤维化的过程就会发展成肝硬化。

背景：前期研究发现，三七总皂苷对小鼠免疫性肝损伤具有一定的保护作用。
目的：探究三七皂苷R1对四氯化碳诱导的肝纤维化模型大鼠的治疗作用。
方法：用四氯化碳诱导SD雄性大鼠制备肝纤维化模型，给药组按照60 mg/kg的剂量给予30 g/L三七皂苷R1溶液， 1次/d，连续4周和6周。对照组及模型组给予同体积的生理盐水，采用苏木精-伊红染色及Masson染色观察肝脏组织结构和纤维化程度分期；反转录-定量聚合酶链反应(qRT-PCR)检测法检测Ⅰ型胶原、α-平滑肌激动蛋白和转化生长因子β1表达水平。实验方案经昆明医科大学动物实验伦理委员会批准(批准号为approval No. KMMU2018018)。
结果与结论：①肝组织病理学显示，与模型组相比，三七皂苷R1能显著减轻纤维增生程度；②与模型组相比，三七皂苷R1组Ⅰ型胶原、α-平滑肌激动蛋白和转化生长因子β1表达水平显著降低(P < 0.05)，三七皂苷R1给药4周与6周组比较差异无显著性意义；③结果提示，三七皂苷R1对四氯化碳诱导的肝纤维化模型大鼠具有一定的治疗作用。
ORCID: 0000-0002-0755-1476(吴朕)

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

关键词: 三七皂苷R1, 四氯化碳, 肝纤维化, Ⅰ型胶原(Col-1), α-SMA, 转化生长因子β1

Abstract:

BACKGROUND: Previous studies have found that panax notoginseng saponins have a certain protective effect on immunological liver injury in mice.

OBJECTIVE: To explore the therapeutic effect of notoginsenoside R1 on carbon tetrachloride-induced liver fibrosis in rats.

METHODS: Experimental liver fibrosis model was made by carbon tetrachloride in male Sprague-Dawley rats. Then 30 g/L notoginsenoside R1 (60 mg/kg) was given once daily for 4 and 6 weeks in the treatment group. Rats in the control and model group were given distilled water of the same volume. Histopathological observation with hematoxylin-eosin staining and Masson’s trichrome staining was used to evaluate the changes of liver structure and fibrosis degree. The expression of collage type I, α-smooth muscle actin and transforming growth factor-β1 mRNA of hepatic tissue was measured by qRT-PCR method. The experimental protocol was approved by the Animal Experiment Ethics Committee of Kunming Medical University (approval No. KMMU2018018).

RESULTS AND CONCLUSION: Liver histopathology showed that notoginsenoside R1 improved the degree of liver fibrosis. The expression levels of collagen type I, α-smooth muscle actin and transforming growth factor-β1 mRNA were reduced significantly in the treatment group compared with the model group (P < 0.05). But there was no significant difference after 4 and 6 weeks of treatment with notoginsenoside R1. Overall findings indicate that notoginsenoside R1 can slow down the progression of carbon tetrachloride-induced liver fibrosis in rats to a certain extent.

Key words: notoginsenoside R1, carbon tetrachloride, liver fibrosis, collagen type I (Col-1), α-smooth muscle actin, transforming growth factor β1

中图分类号: