中国组织工程研究

中国组织工程研究 ›› 2016, Vol. 20 ›› Issue (52): 7878-7884.doi: 10.3969/j.issn.2095-4344.2016.52.018

• 药物控释材料 drug delivery materials • 上一篇    下一篇

烷基化壳聚糖的制备及止血效果

黄玉芬1，邹励宏1，高  洁2，刘  璐3
  

  1. 武警后勤学院，1学员二旅，2科研部，3化学教研室，天津市  300309
  • 收稿日期:2016-09-25 出版日期:2016-12-16 发布日期:2016-12-16
  • 通讯作者: 刘璐，博士，武警后勤学院化学教研室，天津市 300309
  • 作者简介:黄玉芬，女，1994年生，江西省南昌市人，汉族，本科在读，主要从事生物活性材料研究。
  • 基金资助:

    天津市应用基础与前沿技术研究计划一般项目(15JCYBJC18600)

Alkylated-chitosan preparation and its hemostatic effect

Huang Yu-fen1, Zou Li-hong1, Gao Jie2, Liu Lu3
  

  1. 1Second Student Brigade, 2Research Department, 3Department of Chemistry, Logistics University of Chinese Armed Police Force, Tianjin 300309, China
  • Received:2016-09-25 Online:2016-12-16 Published:2016-12-16
  • Contact: Liu Lu, M.D., Department of Chemistry, Logistics University of Chinese Armed Police Force, Tianjin 300309, China
  • About author:Huang Yu-fen, Second Student Brigade, Logistics University of Chinese Armed Police Force, Tianjin 300309, China
  • Supported by:

    the General Project of Research Planning of Tianjin Application Basic and Advanced Technology, No. 15JCYBJC18600

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被引次数

4

摘要：

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文题释义：
壳聚糖：是一种天然碱性多糖，不仅具有生物相容性好、生物活性高的特点，还具有杀菌、诱导红细胞聚集、促进血小板活化及激活补体系统等优点。TraumaStat、Celox和HemCon等以壳聚糖为原料的止血敷料，通过美国FDA认证被用于局部创伤止血治疗。然而最新研究发现，壳聚糖类止血产品对大出血创面的疗效极不稳定，某些产品对股动脉出血创面的止血效果仅与普通脱脂纱布相当。
烷基化壳聚糖：壳聚糖的C2位上引入一定数量的十八烷基苯基团进行疏水改性后，可以显著提高壳聚糖的凝血性能，但是分子中引入烷基必然增加了材料的疏水性，令血液进入材料表面发生困难，因此，引入烷基化的壳聚糖是否能够提高壳聚糖的凝血性能是改性的关键。并不是烷基化程度越高，止血能力越强。因此烷基化程度是考量止血能力较为重要的指标。

背景：最新研究表明，壳聚糖的C2位上引入一定数量的十八烷基苯基团进行疏水改性后，可显著提高壳聚糖的凝血性能。
目的：在壳聚糖分子引入烷基，改善材料与伤口的黏附力。
方法：采用还原氨化法在壳聚糖分子上引入烷基，制备不同取代度的烷基化壳聚糖(7%、16%、26%、40%)，检测壳聚糖与不同取代度烷基化壳聚糖的结构、黏度、孔隙率、吸水率、接触角及体外凝血性能。取30只新西兰白兔，均建立左侧股动脉出血模型，随机分6组，实验4组分别以不同取代度烷基化壳聚糖止血海绵止血，阳性对照组以可吸收止血纱布止血，对照组以壳聚糖止血海绵止血，观察出血量与止血时间。
结果与结论：①随着取代度的增加，烷基化壳聚糖的黏度呈先增加后减小的趋势，孔隙率、吸水率逐渐下降，接触角逐渐增大；②与壳聚糖相比，不同取代度取代度烷基化壳聚糖均具有较好的体外止血效果，其中以16%烷基化壳聚糖的体外止血效果最好；③体内实验显示，取代度16%、26%、40%烷基化壳聚糖组的止血时间与出血量均少于对照组(P < 0.05)，40%烷基化壳聚糖组的出血量少于阳性对照组  (P < 0.05)；④结果表明，取代度40%烷基化壳聚糖用于体内止血时，止血效果最佳。

关键词: 生物材料, 材料相容性, 壳聚糖, 凝血, 烷基化, 止血敷料, 取代度

Abstract:

BACKGROUND: 18-Alkylated-chitosan has been proved to exhibit a good coagulation capacity through hydrophobic modification.
OBJECTIVE: To improve the adhesion between the material and the wound through the introduction of alkylated-chitosan molecules.
METHODS: The alkyl group was introduced in the chitosan by potentiodynamic method to prepare the alkylated-chitosan with different substitution degrees (7%,16%, 26% and 40%), and then their structure, viscosity, porosity, water absorption, contact angle and in vitro coagulation capacity were detected. Thirty New Zealand rabbits were enrolled, modeled into the left femoral artery hemorrhage, and randomized into six groups, including four experimental groups, positive control and control groups, followed by treated with alkylated-chitosan sponges with different degrees of substitution, absorbable hemostatic gauze and chitosan sponge, respectively. Afterwards, the blood loss and clotting time were observed.
RESULTS AND CONCLUISON: With the degree of substitution increased, the viscosity of alkylated-chitosan increased firstly and then decreased, the porosity and water absorption decreased gradually, and the contact angle on a rise. Compared with chitosan, different alkylated-chitosans showed a better henostatic effect in vitro, especially the alkylated-chitosan with 16% substitution degree. The blood loss and clotting time in the experimental groups except that the alkylated-chitosan with 7% substitution degree were significantly lower than those in the control group (P < 0.05); the bold loss in the alkylated-chitosan with 40% substitution degree group was significantly lower than that in the positive control group (P < 0.05). These findings suggest that the alkylated-chitosan with 40% substitution degree exhibits the best hemostatic effect in vivo.

Key words: Chitosan, Hemostasis, Tissue Engineering

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