中国组织工程研究

中国组织工程研究 ›› 2016, Vol. 20 ›› Issue (12): 1718-1724.doi: 10.3969/j.issn.2095-4344.2016.12.007

• 组织工程口腔材料 tissue-engineered oral materials • 上一篇    下一篇

人牙周韧带细胞-聚羟基乙酸支架复合体修复牙周组织缺损

李 敏1，王 瑶2，于华龙3，王双双1，李 蓓1，杨 琳1   

  1. 1河北省中医院，河北省石家庄市 050000；2河北医科大学第一医院，河北省石家庄市  050030；3武邑县医院口腔科，河北省衡水市 053400
  • 收稿日期:2016-01-06 出版日期:2016-03-18 发布日期:2016-03-18
  • 通讯作者: 王瑶，副主任医师，河北医科大学第一医院口腔科，河北省石家庄市 050030
  • 作者简介:李敏，女，1979年生，河北省宁晋县人，硕士，主治医师，主要从事中西医结合治疗牙周炎研究。
  • 基金资助:
    河北省中医药管理局科研计划项目(2014028)

Human periodontal ligament cells-polyglycolic acid scaffold complex for repair of periodontal tissue defects

Li Min1, Wang Yao2, Yu Hua-long3, Wang Shuang-shuang1, Li Bei1, Yang Lin1   

  1. 1TCM Hospital of Hebei Province, Shijiazhuang 050000, Hebei Province, China; 2First Affiliated Hospital of Hebei Medical University, Shijiazhuang 050030, Hebei Province, China; 3Department of Stomatology, Wuyi County Hospital, Hengshui 053400, Hebei Province, China
  • Received:2016-01-06 Online:2016-03-18 Published:2016-03-18
  • Contact: Wang Yao, Associate chief physician, First Affiliated Hospital of Hebei Medical University, Shijiazhuang 050030, Hebei Province, China
  • About author:Li Min, Master, Attending physician, TCM Hospital of Hebei Province, Shijiazhuang 050000, Hebei Province, China
  • Supported by:

    the Scientific Research Project of Hebei Provincial Administration of Traditional Chinese Medicine, No. 2014028

PDF

511

被引次数

5

摘要：

文章快速阅读：

 文题释义：

牙周韧带细胞：是一群具有异质性的多能干细胞，为牙周组织的重要组成部分，具有多向分化潜力，能够在不同酶的催化作用下形成成骨细胞、成纤维细胞，并且可转化为长牙骨质细胞。

聚羟基乙酸支架：是一种简单的聚酯，具有优异的生物可降解性和生物相容性，最终降解产物为二氧化碳和水，通过机体正常的新陈代谢排出体外，是一类重要的医用高分子材料，被广泛应用于骨折内固定、骨缺损修复、肌腱修补及血管和肌肉组织的缝合等。

 

背景：近年来，组织工程技术作为新型组织再生模式，为牙周缺损修复提供了新的思路和方法。
目的：观察人牙周韧带细胞-聚羟基乙酸支架复合体修复牙周组织缺损的效果。
方法：采用多次沉淀法将第4代人牙周韧带细胞以1.5×109 L-1的浓度接种于聚羟基乙酸支架上，制备细胞-支架复合体。取10只杂种犬，建立牙周组织缺损模型后随机分组，实验组牙周组织缺损处植入细胞-支架复合体，对照组牙周组织缺损处直接直接龈瓣冠向复位缝合。术后4周内，检测两组牙周组织缺损处胶原组织、新生毛细血管、新生牙骨质、新生牙槽骨与新生牙周膜组织；术后8周，进行牙周组织缺损处苏木精-伊红染色。

结果与结论：实验组术后1，2，3，4周的胶原含量、新生毛细血管数量、新生牙骨质、新生牙槽骨、新生牙周膜组织均显著多于对照组(P < 0.05)。术后8周，实验组新生牙槽骨的结缔组织面存在较多血管排列，牙槽骨与牙周膜的链接呈锯齿状，可看见薄层牙骨质沉积；对照组仅见新生牙槽骨和牙骨质形成，部分位于切迹以下。结果表明，人牙周韧带细胞-聚羟基乙酸支架复合体可促进牙周组织再生。 

ORCID: 0000-0002-8012-071X(李敏)

关键词: 生物材料, 口腔生物材料, 聚羟基乙酸支架, 人牙周韧带细胞, 牙周组织缺损, 修复效果, 新生毛细血管

Abstract:

BACKGROUND: In recent years, tissue engineering technology as a new model for tissue regeneration has provided new ideas and methods for the repair of periodontal tissue defects.
OBJECTIVE: To investigate the effect of human periodontal ligament cells-polyglycolic acid scaffold complex for repair of periodontal tissue defects.
METHODS: Passage 4 human periodontal ligament cells at a density of 1.5×109/L were seeded onto the polyglycolic acid scaffold to prepare cell-scaffold complex. Then mongrel dogs were selected to make animal models of periodontal tissue defects and then randomly assigned into experimental group subjected to cell-scaffold complex implantation or control group subjected to direct coronal reset and suture of the gingival flap. Collagen content, new blood capillaries, new cementum, new alveolar bone and new periodontal ligament were detected within 4 weeks after operation; hematoxylin-eosin staining of periodontal tissue defects was done at 8 weeks after operation.
RESULTS AND CONCLUSION: In the experimental group, the collagen content, number of newborn capillaries, amount of new cementum, new alveolar bone and new periodontal ligament tissues were significantly higher than those in the control group at postoperative 1, 2, 3, 4 weeks (P < 0.05). At 8 weeks after operation, in the experimental group, there were more vessels arranging on the connective tissue surface of new alveolar bone, the alveolar bone showed a sawtooth-like interlinking with the periodontal tissues in the presence of a thin layer of cementum; in the control group, only new alveolar bone and cementum formed below the incisure. These findings indicate that human periodontal ligament cells-polyglycolic acid scaffold complex can promote periodontal tissue regeneration.