中国组织工程研究

中国组织工程研究 ›› 2015, Vol. 19 ›› Issue (45): 7337-7341.doi: 10.3969/j.issn.2095-4344.2015.45.022

• 干细胞培养与分化 stem cell culture and differentiation • 上一篇    下一篇

促红细胞生成素体外诱导神经干细胞的分化

赵 岩1，王喜良1，肖宇龙1，霍洪军1，江建明2，闫慧博3   

  1. 1内蒙古医科大学第二附属医院脊柱外科，内蒙古自治区呼和浩特市 010030；2南方医科大学附属南方医院脊柱骨科外科，广东省广州市 510515；3南方医科大学第三附属医院脊柱外科，广东省广州市 510630
  • 收稿日期:2015-09-25 出版日期:2015-11-05 发布日期:2015-11-05
  • 通讯作者: 肖宇龙，主任医师，内蒙古医科大学第二附属医院脊柱外科，内蒙古自治区呼和浩特市 010030
  • 作者简介:赵岩，男，1977年生，内蒙古自治区赤峰市人，汉族，2010年南方医科大学毕业，博士，副主任医师，主要从事脊髓损伤的基础和临床研究。
  • 基金资助:

    内蒙古自治区科技厅自然科学基金资助课题(2011MS1139)

Differentiation of neural stem cells induced by erythropoietin in vitro

Zhao Yan1, Wang Xi-liang1, Xiao Yu-long1, Huo Hong-jun1, Jiang Jian-ming2, Yan Hui-bo3   

  1. 1Department of Thoracolumbar Spine Surgery, the Second Affiliated Hospital of Inner Mongolia Medical University, Hohhot 010030, Inner Mongolia Autonomous Region, China; 2Department of Orthopedic and Spinal Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong Province, China; 3Department of Spinal Surgery, Third Affiliated Hospital, Southern Medical University, Guangzhou 510630, Guangdong Province, China
  • Received:2015-09-25 Online:2015-11-05 Published:2015-11-05
  • Contact: Xiao Yu-long, Chief physician, Department of Thoracolumbar Spine Surgery, the Second Affiliated Hospital of Inner Mongolia Medical University, Hohhot 010030, Inner Mongolia Autonomous Region, China
  • About author:Zhao Yan, M.D., Associate chief physician, Department of Thoracolumbar Spine Surgery, the Second Affiliated Hospital of Inner Mongolia Medical University, Hohhot 010030, Inner Mongolia Autonomous Region, China
  • Supported by:

    the Natural Science Foundation of Scientific Committee of Inner Mongolia Autonomous Region, No. 2011MS1139

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摘要：

背景：近年来，神经干细胞被认为是治疗脊髓损伤的理想移植细胞，但其在宿主体内自然分化为神经元的比例较低，严重制约了其修复脊髓损伤的效果。
目的：分析促红细胞生成素体外诱导神经干细胞分化的作用。
方法：从新生 Wistar 大鼠海马组织中无菌条件下分离神经干细胞，进行体外分离、培养及免疫荧光鉴定。然后取第3代的神经干细胞，随机分为0.5，5，50 U/mL促红细胞生成素组和对照组，分别加入0.5，5，50，0 U/mL促红细胞生成素。
结果与结论：0.5，5，50 U/mL促红细胞生成素组神经元的转化率较对照组明显提高(P < 0.05)，5，50 U/mL促红细胞生成素组神经元的转化率高于0.5 U/mL促红细胞生成素组(P < 0.05)，但5，50 U/mL促红细胞生成素组中神经元数量接近(P > 0.05)。说明促红细胞生成素在体外能够有效地诱导神经干细胞向神经元的分化，并可明显提高其向神经元的转化率。
中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

关键词: 干细胞, 分化, 促红细胞生成素, 神经干细胞, 神经元, 脊髓损伤, 培养, 诱导, 转化率

Abstract:

BACKGROUND: In recent years, neural stem cells are considered to be ideal for the treatment of spinal cord injury, but the proportion of its natural differentiation into neurons in the host body is relatively low, which severely restricts the therapeutic effect on spinal cord injury.
OBJECTIVE: To investigate the effect of erythropoietin on the differentiation of neural stem cells in vitro.
METHODS: Under sterile condition, neural stem cells from the hippocampus of neonatal Wistar rats were isolated, cultured and identified by immunofluorescence in vitro. The third generation of neural stem cells were randomly divided into 0.5, 5, 50 U/mL erythropoietin groups and control group (with no erythropoietin).
RESULTS AND CONCLUSION: Compared with the control group, the differentiation rate of neural stem cells was significantly improved in the 0.5, 5, 50 U/mL erythropoietin groups (P < 0.05); moreover, the differentiation rate of neural stem cells in the 5, 50 U/mL erythropoietin groups was higher than that in the 0.5 U/mL erythropoietin group (P < 0.05). In addition, there was no significant difference between the 5, 50 U/mL erythropoietin groups (P > 0.05). These findings indicate that erythropoietin can effectively induce the  differentiation of neural stem cells into neurons in vitro, and moreover, it can significantly improve the differentiation rate of neural stem cells into neurons.
中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Erythropoietin, Neural Stem Cells, Cell Differentiation, Tissue Engineering