中国组织工程研究

中国组织工程研究 ›› 2015, Vol. 19 ›› Issue (18): 2838-2842.doi: 10.3969/j.issn.2095-4344.2015.18.009

• 器官移植动物模型 organ transplantation and animal model • 上一篇    下一篇

构建不同周龄载脂蛋白E基因敲除动脉粥样硬化模型小鼠血脂和病理学观察

谢  佳，陈清杰，杨毅宁，马依彤，李晓梅，刘  芬，陈邦党，翟  慧，周  韵   

  1. 新疆医科大学第一附属医院心脏中心，新疆维吾尔自治区乌鲁木齐市  830054
  • 收稿日期:2015-03-19 出版日期:2015-04-30 发布日期:2015-04-30
  • 通讯作者: 杨毅宁，博士，主任医师，教授，博士生导师，新疆医科大学第一附属医院心脏中心，新疆维吾尔自治区乌鲁木齐市 830054
  • 作者简介:谢佳，女，1989年生，河南省西平县人，汉族，新疆医科大学在读硕士，主要从事冠状动脉粥样硬化性心脏病基础及临床方面的研究。
  • 基金资助:

    国家自然科学基金项目(81160042，81460069)

Serum lipid levels and pathological observation of apolipoprotein E knockout mice with atherosclerosis at different weeks of age 

Xie Jia, Chen Qing-jie, Yang Yi-ning, Ma Yi-tong, Li Xiao-mei, Liu Fen, Chen Bang-dang, Zhai Hui,Zhou Yun   

  1. Heart Center, First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China
  • Received:2015-03-19 Online:2015-04-30 Published:2015-04-30
  • Contact: Yang Yi-ning, M.D., Chief physician, Professor, Doctoral supervisor, Heart Center, First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China
  • About author:Xie Jia, Studying for master’s degree, Heart Center, First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China
  • Supported by:
     the National Natural Science Foundation of China, No. 81160042, 81460069
     

PDF

703

被引次数

2

摘要：

背景：载脂蛋白E基因敲除小鼠形成的动脉粥样硬化病变与人类全身动脉粥样硬化好发处相近，是目前建立动脉粥样硬化理想的动物模型。
目的：研究载脂蛋白E基因敲除小鼠不同周龄动脉粥样硬化的病理进程，探讨不同饮食对载脂蛋白E基因敲除小鼠动脉粥样硬化发生发展的影响。
方法：将8周龄雄性载脂蛋白E基因敲除小鼠，随机分为2组，分别给予高脂饮食和普通饮食喂养8，12，16，20，24周。
结果与结论：血清学指标检测显示，不同周龄的高脂饮食组血清中总胆固醇、三酰甘油和低密度脂蛋白胆固醇水平显著高于普通饮食组(P < 0.05)，呈时间依赖性。大体和冰冻切片油红O染色结果显示，高脂饮食组动脉粥样硬化管腔斑块面积显著高于普通饮食组(P < 0.05)，呈时间依赖性，此时两组各周龄小鼠管腔斑块面积相比均有显著性意义(P < 0.05)，小鼠在高脂饮食16周时主动脉可见明显的脂质斑块。结果表明，实验成功构建了载脂蛋白E基因敲除动脉粥样硬化模型小鼠，此模型形成脂质条纹和纤维增生病变的时间较普通饮食组更快。



中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程


全文链接：

关键词: 实验动物, 心脏及血管损伤模型, 载脂蛋白E基因敲除小鼠, 动脉粥样硬化, 高脂饮食, 主动脉, 油红O染色, 国家自然科学基金

Abstract:

BACKGROUND: The formation of atherosclerotic lesions in apolipoprotein E knockout mice is similar to that of human systemic atherosclerosis, and apolipoprotein E knockout mice are ideal animals for current establishment of atherosclerosis models.
OBJECTIVE: To research the pathological process of atherosclerosis in apolipoprotein E knockout mice aged different weeks, and to explore the effect of different diets on the occurrence and development of atherosclerosis in apolipoprotein E knockout mice. 
METHODS: Male apolipoprotein E knockout mice aged 8 weeks old were randomly divided into two groups, and fed with high fat diet and normal diet, respectively, for 8, 12, 16, 20, and 24 weeks.
RESULTS AND CONCLUSION: Serological detection revealed that serum total cholesterol, triglycerides and low density lipoprotein levels were significantly higher in different weeks of mice of high fat diet group than in the normal diet group (P < 0.05), in a time-dependent manner. Gross and frozen oil red O staining showed that atherosclerotic plaque area of lumen was significantly larger in the high fat diet group than in the normal diet group (P < 0.05), in a time-dependent manner. At this time, significant differences in plaque area of lumen at each week were detected 
between both groups (P < 0.05). Apparent lipid plaque was visible in aorta at 16 weeks of high fat diet in mice. Results demonstrated that apolipoprotein E knockout mice of atherosclerosis were successfully established. The formation of lipid streaks and fiber hyperplasia was faster in high fat diet group than in the normal diet group.



中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程


全文链接：

Key words: Models, Animals, Atherosclerosis, Serum Lipid, Pathology

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