中国组织工程研究

中国组织工程研究 ›› 2015, Vol. 19 ›› Issue (14): 2144-2148.doi: 10.3969/j.issn.2095-4344.2015.14.003

• 骨髓干细胞 bone marrow stem cells • 上一篇    下一篇

富血小板血浆促进骨髓间充质干细胞的增殖

徐 翔1，银和平2   

  1. 1内蒙古医科大学，内蒙古自治区呼和浩特市 010059；2内蒙古医科大学第二附属医院微创脊柱外科，内蒙古自治区呼和浩特市 010030
  • 修回日期:2015-03-14 出版日期:2015-04-02 发布日期:2015-04-02
  • 通讯作者: 银和平，教授，主任医师，硕士生导师，内蒙古医科大学第二附属医院微创脊柱外科，内蒙古自治区呼和浩特市 010030
  • 作者简介:徐翔，男，1988年生，内蒙古自治区呼和浩特市人，汉族，内蒙古医科大学在读硕士，主要从事骨科专业方面的研究。
  • 基金资助:

    国家自然科学基金(81260287)

Platelet-rich plasma accelerates the proliferation of bone marrow mesenchymal stem cells

Xu Xiang1, Yin He-ping2   

  1. 1Inner Mongolia Medical University, Hohhot 010059, Inner Mongolia Autonomous Region, China; 2Department of Spinal Surgery, Second Affiliated Hospital of Inner Mongolia Medical University, Hohhot 010030, Inner Mongolia Autonomous Region, China
  • Revised:2015-03-14 Online:2015-04-02 Published:2015-04-02
  • Contact: Yin He-ping, Professor, Chief physician, Master’s supervisor, Department of Spinal Surgery, Second Affiliated Hospital of Inner Mongolia Medical University, Hohhot 010030, Inner Mongolia Autonomous Region, China
  • About author:Xu Xiang, Studying for master’s degree, Inner Mongolia Medical University, Hohhot 010059, Inner Mongolia Autonomous Region, China
  • Supported by:

    the National Natural Science Foundation of China, No. 81260287

PDF

478

被引次数

3

摘要：

背景：富血小板血浆含有多种刺激因子，且能上调蛋白多糖及胶原合成。

目的：观察山羊富血小板血浆对山羊骨髓间充质干细胞增殖的影响。
方法：抽取6月龄的内蒙古锡盟山羊颈静脉血，采用二度离心法获取富血小板血浆，运用骨髓穿刺包从山羊髂骨上抽取骨髓，采用密度梯度离心方法分离、纯化获得山羊骨髓间充质干细胞，然后取生长状态较好的原代细胞，加入含体积分数为10%，20%，30%富血小板血浆的L-DMEM完全培养基进行培养，对照组只用L-DMEM完全培养基培养。
结果与结论：在2-6 d期间，富血小板血浆各组明显比对照组细胞增殖迅速，而且体积分数越高细胞生长的速度越快，生长的数目越多，细胞形态越成熟。培养第4天时，体积分数为10%，20%，30%富血小板血浆组细胞倍增时间约为50 h，35 h，25 h。结果表明山羊富血小板血浆能明显促进骨髓间充质干细胞的增殖，而且富血小板血浆体积分数越大促增殖能力越显著。

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程


全文链接：

关键词: 干细胞, 骨髓干细胞, 山羊, 骨髓间充质干细胞, 富血小板血浆, 增殖, 国家自然科学基金

Abstract:

BACKGROUND: Platelet-rich plasma contains a variety of stimulating factors, and can also raise the proteoglycan and collagen synthesis.

OBJECTIVE: To observe the effect of platelet-rich plasma on the proliferation of goat bone marrow mesenchymal stem cells.
METHODS: Blood samples were extracted from the jugular vein of Inner Mongolia Ximeng goats to harvest platelet-rich plasma using centrifugation method. Then, bone marrow was extracted from the goat’s ilium by puncture method to isolate and purify goat bone marrow mesechymal stem cells using density gradient centrifugation method. After that, primary cells at good state were cultured in L-DMEM complete medium containing 10%, 20%, 30% platelet-rich plasma or in simple L-DMEM complete medium.
RESULTS AND CONCLUSION: Within 2-6 days of culture, cells in the platelet-rich plasma groups proliferated faster than those in the control group, and with the increasing of platelet-rich plasma concentration, the cells grew faster, with larger number and more mature morphology. At 4 days of culture, the cell doubling time was about 50, 35, 25 hours in the 10%, 20%, and 30% platelet-rich plasma groups, respectively. These findings indicate that goat platelet-rich plasma can dramatically promote the proliferation of bone marrow mesenchymal stem cells in a concentration-dependent manner.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程


全文链接：

Key words: Bone Marrow, Mesenchymal Stem Cells, Platelet-Rich Plasma, Cell Proliferation, Goats

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