中国组织工程研究

中国组织工程研究 ›› 2014, Vol. 18 ›› Issue (30): 4771-4776.doi: 10.3969/j.issn.2095-4344.2014.30.003

• 组织工程骨及软骨材料 tissue-engineered bone and cartilage materials • 上一篇    下一篇

电纺丝技术制备组织工程食管仿生支架

於学婵，沈秋霞，卢珍珍，张  陈，邓  玲，胡  品，竺亚斌   

  1. 宁波大学医学院，浙江省宁波市  315211
  • 修回日期:2014-06-14 出版日期:2014-07-16 发布日期:2014-08-08
  • 通讯作者: 竺亚斌，教授，宁波大学医学院，浙江省宁波市 315211
  • 作者简介:於学婵，女，1993年生，安徽省六安市寿县人，汉族，宁波大学医学院本科在读。
  • 基金资助:

    国家自然科学基金(81171476)；宁波市创新团队项目(2011B82014)；宁波市社发项目(2013C50031)

Preparation of tissue-engineered esophageal scaffolds using electrospinning technology

Yu Xue-chan, Shen Qiu-xia, Lu Zhen-zhen, Zhang Chen, Deng Ling, Hu Pin, Zhu Ya-bin   

  1. Medical School of Ningbo University, Ningbo 315211, Zhejiang Province, China
  • Revised:2014-06-14 Online:2014-07-16 Published:2014-08-08
  • Contact: Zhu Ya-bin, Professor, Medical School of Ningbo University, Ningbo 315211, Zhejiang Province, China
  • About author:Yu Xue-chan, Medical School of Ningbo University, Ningbo 315211, Zhejiang Province, China
  • Supported by:

    the National Natural Science Foundation of China, 81171476; the Innovative Team Program of Ningbo City, No. 2011B82014; the Social Development Project of Ningbo City, No. 2013C50031

PDF

801

被引次数

2

摘要：

背景：前期实验中曾发现纤维的取向可以引导平滑肌细胞的取向生长，因此，设想通过制备取向排列的电纺丝纤维支架，以引导食管平滑肌细胞的有序生长，从而有利于维持肌细胞的形貌及生物功能。
目的：以可降解聚己内酯、明胶、丝素蛋白为基材，采用自制的电纺丝系统制备无规和有序的纳米级多孔纤维。
方法：将聚己内酯与丝素蛋白以4∶1质量比混合，通过调整溶液浓度、电压、喷射速度等参数，采用自制的电纺丝系统制备聚己内酯/丝素蛋白电纺丝纤维。将聚己内酯与明胶分别以2∶1、1∶1、1∶2质量比混合，在金属平板接收器下，通过调整溶液浓度、电压、喷射速度等参数，采用自制的电纺丝系统制备聚己内酯/明胶无规电纺丝纤维；同时改用滚轴接收装置，通过调整滚轴转数、电压、喷射速度等参数，制备聚己内酯/明胶有序电纺丝纤维。
结果与结论：在溶液质量浓度为0.08 g/mL、纺丝液流速1.6 mL/h和电压22.5 kV的条件下，制得了均匀、无串珠、纤维直径为(535.9±126.7) nm的聚己内酯/丝素蛋白多孔纳米纤维膜。在溶液质量浓度为0.10 g/mL、纺丝液流速0.8 mL/h和电压22.5 kV的条件下，制得了无明显串珠、纤维直径为(257.9±117.8) nm的聚己内酯/明胶多孔纳米纤维膜；并且在1∶2质量比时更易成纤维，纤维尺寸更均匀。在滚轴转速3 000 r/min。溶液流速0.8 mL/h。电压15 kV的条件下，制得的聚己内酯/明胶有序电纺丝纤维排序更理想，纤维也更均匀。


中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程


全文链接：

关键词: 生物材料, 纳米材料, 电纺丝, 聚己内酯, 纳米纤维, 取向, 食管组织工程, 仿生支架, 国家自然科学基金

Abstract:

BACKGROUND: We have found that oriented fibers can guide the alignment of smooth muscle cells in our previous experiments. Thus, we designed the experiment to prepare well aligned polymeric fibers using electrospinning technology, aiming at guiding the growth of esophageal smooth muscle cells to maintain cell morphology and biological function.
OBJECTIVE: Using electrospinning technology, to fabricate isotropic and directed nano-fibrous scaffolds made of polycaprolacton, gelatin and silk fibroin.
METHODS: Polycaprolacton/silk fibroin fibers at a ratio of 4:1 were prepared with proper parameters, including solution concentration, voltage and injection speed, under the self-made spinning system. The polycaprolacton/ gelatin sheets with mass ratio of 2:1, 1:1 and 1:2, respectively, were also fabricated under suitable process parameters. Using the roller collector instead of the metal plate, polycaprolacton/gelatin nano-fibrous scaffold with good alignment of fibers was manufactured.
RESULTS AND CONCLUSION: The isotropic polycaprolacton/silk fibroin scaffold with fiber diameter of (535.9±126.7) nm was prepared under conditions of solution concentration (0.08 g/mL), injection speed (1.6 mL/h) and voltage (22.5 kV), and these fibers were uniform with no beads. The isotropic polycaprolacton/gelatin scaffold with fiber diameter of (257.9±117.8) nm was prepared under conditions of solution concentration (0.10 g/mL), injection speed (0.8 mL/h) and voltage (22.5 kV). Using the roller collector instead of the previous metal plate, polycaprolacton/ gelatin (w:w, 1:2) nano-fibrous scaffold with good alignment of fibers was manufactured. The process parameters were  3 000 r/min of rolling speed, 0.8 mL/h of injection speed and 15 kV of voltage.


中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程


全文链接：

Key words: nanofibers, gelatin, silk

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