中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (52): 9787-9791.doi: 10.3969/j.issn.2095-4344.2012.52.019

• 骨与关节损伤基础实验 basic experiments of bone and joint injury • 上一篇    下一篇

活血通络法治疗激素性股骨头坏死兔胰岛素样生长因子1的变化

张 弛1，杨志伟2，段小英1   

  1. 1成都中医药大学，四川省成都市 610075
    2雅安职业技术学院，四川省雅安市 625000
  • 收稿日期:2012-05-04 修回日期:2012-10-08 出版日期:2012-12-23 发布日期:2012-12-23
  • 通讯作者: 杨志伟，讲师，雅安职业技术学院，四川省雅安市 625000 yayzw@126.com
  • 作者简介:张弛★，男，1973年生，四川省广安市人，汉族，1998年成都中医药大学毕业，硕士，副教授，主要从事骨与关节损伤重建与修复的研究。zhangchid@126.com

Changes of insulin-like growth factor 1 levels in rabbits with steroid-induced femoral head necrosis after treatment with Huoxuetongluo method

Zhang Chi1, Yang Zhi-wei2, Duan Xiao-ying1   

  1. 1Chengdu University of Traditional Chinese Medicine, Chengdu 610075, Sichuan Province, China
    2Ya'an Vocational College, Ya’an 625000, Sichuan Province, China
  • Received:2012-05-04 Revised:2012-10-08 Online:2012-12-23 Published:2012-12-23
  • Contact: Yang Zhi-wei, Lecturer, Ya'an Vocational College, Ya’an 625000, Sichuan Province, China yayzw@126.com
  • About author:Zhang Chi★, Master, Associate professor, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, Sichuan Province, China zhangchid@126.com

PDF

434

被引次数

18

摘要：

背景：活血通络法是临床防治激素性股骨头坏死的有效治法，活血通络方促进骨坏死组织修复有确切疗效。
目的：观察活血通络法对激素性股骨头坏死家兔模型组织形态学和胰岛素样生长因子1的影响。
方法：家兔随机分为5组，其中4组用糖皮质激素造成激素性股骨头坏死模型，活血通络方高、中、低剂量组分别灌服活血通络汤混悬液10.0，5.0，2.5 mL/kg，模型组灌服10 mL/kg蒸馏水；空白对照组不造模仅灌服10 mL/kg蒸馏水，连续8周。
结果与结论：组织形态学检查显示，模型组骨坏死发生严重，活血通络方各剂量组骨坏死发生轻。空骨陷窝率模型组较空白对照组明显升高(P < 0.01)，活血通络方各剂量组较模型组明显降低(P < 0.05)。胰岛素样生长因子1水平模型组较空白对照组升高(P < 0.05)，活血通络方各剂量组均较模型组明显升高(P < 0.01)，但活血通络方中高剂量组之间差异无显著性意义(P > 0.05)。提示活血通络方可能通过升高胰岛素样生长因子1水平来促进坏死股骨头的修复与重建，但当剂量达到一定程度时胰岛素样生长因子1并不随剂量的增大而继续升高。

关键词: 活血通络法, 中药治疗, 激素性股骨头坏死, 胰岛素样生长因子1, 组织形态, 空骨陷窝率, 骨坏死, 糖皮质激素, 组织构建

Abstract:

BACKGROUND: Huoxuetongluo method is an effective prescription for the treatment of steroid induced femoral head necrosis in clinic, and Huoxuetongluo can promote osteonecrosis tissue repair.
OBJECTIVE: To investigate the effect of Huoxuetongluo method on the level of insulin-like growth factor-1 in the steroid induced femoral head necrosis rabbit model.
METHODS: The rabbits were randomly divided into five groups, and the rabbits in four groups were used to establish the steroid induced femoral head necrosis model through injecting the glucocorticoid. The rabbits in the high, moderate and low dose groups were administered intramuscularly with 10.0, 5.0 and 2.5 mL/kg Huoxuetongluo decoction, rabbits in both the model group and the blank control group were administered intramuscularly with 10 mL/kg normal saline and lasted for 8 weeks.
RESULTS AND CONCLUSION: Histomorphometric examination showed that the femoral head necrosis in the model group was severe, while the femoral head necrosis in the high, moderate and low dose groups was mild necrosis. The data of empty lacuna in the model group was significantly higher than that in the blank control group (P < 0.01), and the level in the high, moderate and low dose groups was lower than that in the model group (P < 0.05); the level of insulin-like growth factor-1 in the model group was higher than the in the blank control group (P < 0.05) and the level in the high, moderate and low dose groups was higher than that in the model group (P < 0.01), while there was no significant difference between the every two Huoxuetongluo groups (P > 0.05). Huoxuetongluo decoction can promote the repairing and reconstruction of femoral head necrosis through increasing the level of insulin-like growth factor-1, but the level of insulin-like growth factor-1 will not increased with the increasing of the dose of Huoxuetongluo decoction when the dose reached to a certain level.

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